Research

  • investigation of subcellular GCPR-signalling in cell culture, organotypic environment and in living animals
  • Characterization of specific G-proteins and downstream effectors involved in GPCR-mediated signal, cross-talk and feedback mechanisms
  • Post-translational modifications of the signaling proteins and their role in the regulation of the signal transduction
  • ...

In our experiments we apply all current methods of molecular and cell biology as well as protein biochemistry and receptor pharmacology. In addition, we possess broad experiences in electrophysiology (patch-clamp), time-lapse microscopy and fluorescence microscopy. I our lab we also established the modern biophysical approaches for molecular imaging including, Förster resonance energy transfer (FRET) in cuvette and at single-cell level, fluorescence life-time measurements (FLIM) as well as bioluminescence resonance energy transfer (BRET). To analyse the expression level of different proteins we routinely use the quantitative RT-PCR analysis (TaqMan and SYBR Green) as well as single-cell RT-PCR techniques. 


Our Projects