Dr. Karsten Cirksena
Team of Prof. Dr. Falk Büttner
Mailing Address: Medizinische Hochschule Hannover, MHH
Institut für Klinische Biochemie, OE 4340
Building J03, Level 04, Room 1300 (Office), Room 1250 (Lab)
Karsten is a postdoctoral fellow and molecular biologist combining genetic engineering and different omics data derived from a variety of mammalian cell types. He studied Biology at the University of Hamburg where he got his Bachelor degree with a study on the regulation of bone remodelling at the Helmholtz‑Centre hereon. He moved to the Hannover Medical School to study Biomedicine and later joined the Institute of Clinical Biochemistry to establish the genetic engineering in stem cells and proteomics data analyses, ending up with a Master degree and a PhD as part of the Regenerative Sciences program of the REBIRTH Cluster of Excellence.
C‑mannosylation is a barely studied modification of proteins by a single mannose, which is catalysed by two known C‑mannosyltransferases (C‑manTs) DPY19L1 and DPY19L3 and can affect the folding, stability or secretion of proteins. Although it was predicted for a multitude of secreted proteins, to date, only few proteins are known to be C‑mannosylated. Thus, the actual function of this modification is hardly understood. However, it was shown that the lack of C‑mannosylation can affect early processes during embryonic development.
The main emphasis of my research focusses on (I) the design of C‑manT‑deficient human stem cells applying the CRISPR‑Cas system, (II) the discovery of new candidates for C‑mannosylation by MS‑based secretome analyses of these cells, (III) the evaluation of our findings by recombinant protein expression, purification and (targeted) MS analysis and (IV) investigating the meaning of our discoveries for cell signalling during early embryonic development.
- Glycobiology (C‑mannosylation)
- Mass Spectrometry
- Big Data Analytics (Omics)
- Stem Cell Biology
- Genetic Engineering (CRISPR‑Cas)
CIRKSENA, K., HUTTE, H. J., SHCHERBAKOVA, A., THUMBERGER, T., SAKSON, R., WEISS, S., JENSEN, L. R., FRIEDRICH, A., TODT, D., KUSS, A. W., RUPPERT, T., WITTBRODT, J., BAKKER, H. & BUETTNER, F. F. R. 2021. The C-mannosylome of human induced pluripotent stem cells implies a role for ADAMTS16 C-mannosylation in eye development. Mol Cell Proteomics, 100092.
DETTMER, R. †, CIRKSENA, K. †, MUNCHHOFF, J. †, KRESSE, J., DIEKMANN, U., NIWOLIK, I., BUETTNER, F. F. R. & NAUJOK, O. 2020. FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells, 9.
- Lectures and supervision of laboratory courses within the chemistry module of the medical study program HannibaL, Hannover Medical School
- Supervision of Bachelor and Master theses
- Seminars on analysis of proteomics data based on MaxQuant and Perseus software