Judith Montag

Dr. rer. nat.

Kontakt

Judith Montag

Tel:  +49 511 532 -2094 (Büro), -3654 (Labor)

Fax: +49 511 532 -4296

Adresse: 30625 Hannover, Carl-Neuberg-Str.1

Büro: Gebäude J03, Block 01, Ebene 03, Raum 1340

 

 

 

 

 

 

 

Werdegang

  • Seit 2011 wissenschaftliche Mitarbeiterin und Arbeitsgruppenleiterin im Institut für Molekular- und Zellphysiologie an der Medizinischen Hochschule Hannover (MHH)
  • 2009 bis 2010 Arbeitsgruppenleiterin AG „Herpesviren von Makakken“ in der Abteilung Infektionsbiologie am Deutschen Primatenzentrum (DPZ) in Göttingen
  • 2007 bis 2009 PostDoc in der AG „Herpesviren von Makakken“ in der Abteilung Virologie und Immunologie am Deutschen Primatenzentrum (DPZ) in Göttingen
  • 2004 bis 2007 in der Arbeitsgruppe „BSE - Risk assessment” in der Abteilung für Virologie and Immunologie, Deutsches Primatenzentrum, Göttingen. Titel der Arbeit: „Übertragung von BSE auf nicht humane Primaten als Modell für die variante Creutzfeldt-Jakob Erkrankung im Menschen“
  • 1999 bis 2004 Biologie (Hauptfach Mikrobiologie) mit Abschluss „Diplom“ an der Georg-August-Universität in Göttingen
  • 1999: Abitur am Gymnasium Nepomucenum in Rietberg

 

Aktuelle Forschungsschwerpunkte

  • Unterschiedliches Expressionsverhältnis von mutiertem zu Wildtyp-Allel in einzelnen Kardiomyozyten als neues Konzept zur Pathogenese der Familiären Hypertrophen Kardiomyopathie
  • Genomeditierte Schweine als Großtiermodel für die Hypertrophe Kardiomyopathie
  • Analyse der burst-like Transkription von HCM-assoziierten Genen

 

Expertisen

  • Wissenschaftliche Methoden: Molekularbiologische Methoden (besonderer Fokus: RNA Expressionsanalysen, Klonierungen, Einzelzellanalysen, Genomeditierung durch Designernukleasen TALEN und CRISPR/Cas9), Zellkultur, Virologische Methoden und Umgang mit Viren der Sicherheitsstufen 1 bis 3 (Umgangsgenehmigung nach IfSG §44), Tiermodelle (nicht humane Primaten, Schwein), Biochemische Methoden, Expression rekombinanter Proteine im Baculovirussystem
  • Betreuung von Bachelorarbeiten, Diplomarbeiten, Doktorarbeiten in der Biologie, Biochemie und Medizin  …
  • Lehre: Studentische Praktika (Auge, Blut, Herz, Muskel, Ohr), Seminare (Atmung, Auge, Blut, Herz, Muskel, Ohr, ZNS) für Mediziner, Zahnmediziner und Biologen

 

Wissenschaftliche Artikel in Fachzeitschriften

2019

  • Altered force generation and cell-to-cell contractile imbalance in hypertrophic cardiomyopathy; Kraft T, Montag J; Pflügers Arch: 471(5):719-733; 2019 May; doi: 10.1007/s00424-019-02260-9; Review

2018

  • Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene; Gerlach M, Kraft T, Brenner B, Petersen B, Niemann H, Montag J.. Genes (Basel):13;9(6); 2018 Jun; doi: 10.3390/genes906029
  • Burst-like transcription of mutant and wildtype MYH7-alleles as possible origin of cell-to-cell contractile imbalance in hypertrophic cardiomyopathy; Montag J, Kowalski K, Makul M, Ernstberger P, Radocaj A, Beck J, Becker E, Tripathi S, Keyser B, Mühlfeld C, Wissel K, Pich A, van der Velden J, dos Remedios CG, Perrot A, Francino A, Navarro-López F, Brenner B, Kraft T; Front Physiol: 9:359. 2018 Apr; doi: 10.3389/fphys.2018.00359
  • Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs; Montag J, Petersen B, Lucas-Hahn A, Becker E, Harries D, Niemeier H, Brenner B, Kraft T; Sci Rep: 8(1):4786; 2018 Mar; doi: 10.1038/s41598-018-22936-z

2017

  • Intrinsic MYH7 expression regulation contributes to tissue level allelic imbalance in hypertrophic cardiomyopathy; Montag J, Syring M, Rose J, Weber AL, Ernstberger P, Mayer AK, Becker E, Keyser B, Dos Remedios C, Perrot A, van der Velden J, Francino A, Navarro-Lopez F, Ho CY, Brenner B, Kraft T; J Muscle Res Cell Motil: 38 (3-4):291-302; 2017 Aug; doi: 10.1007/s10974-017-9486-4.

2016

  • Kraft T, Montag J, Radocaj A, Brenner B. Hypertrophic Cardiomyopathy: Cell-to-Cell Imbalance in Gene Expression and Contraction Force as Trigger for Disease Phenotype Development; Circ Res: 119(9):992-995; 2016 Oct; doi: 10.1161/CIRCRESAHA.116.309804

2014

  • Familial hypertrophic cardiomyopathy: functional variance among individual cardiomyocytes as a trigger of FHC-phenotype development; Brenner B, Seebohm B, Tripathi S, Montag J, Kraft T; Front Physiol: 5:392; 2014 Oct; 10.3389/fphys.2014.00392
  • Faster cross-bridge detachment and increased tension cost in human hypertrophic cardiomyopathy with the R403Q MYH7 mutation. Witjas-Paalberends ER, Ferrara C, Scellini B, Piroddi N, Montag J, Tesi C, . Stienen GJM, Michels M, Ho CY, Kraft T, Poggesi C,van der Velden J; J Physiol: 592(Pt 15):3257-72; 2014 Aug; doi: 10.1113/jphysiol.2014.274571
  • Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques; Barbisin M, Vanni S, Schmädicke AC, Montag J, Motzkus D, Opitz L, Salinas-Riester G, Legname G; BMC Genomics:15:434, 2014 Jun; doi: 10.1186/1471-2164-15-434

2013

  • Mapping the Small RNA Content of Simian Immunodeficiency Virions (SIV); Brameier M, Ibing W, Höfer K, Montag J, Stahl-Hennig C, Motzkus D; PLoS One: 8(9): e75063; 2013 Sept; doi: 10.1371/journal.pone.0075063
  • Asynchronous onset of prion disease in bovine spongiform encephalopathy (BSE) infected macaques; Montag J, Schulz-Schäffer W, Schrod A, Hunsmann G, Motzkus D; Emerg Infect Dis: 19(7):1125-7;2013 Jul; doi: 10.3201/eid1907.120438
  • Familial Hypertrophic Cardiomyopathy: functional effects of myosin mutation R723G in cardiomyocytes; Kraft T, Paalberends ER, Boontje N, Tripathi S, Brandis A, Montag J, Francino A, Navarro-Lopez F, Brenner B, Stienen GJM, van der Velden J; J Mol Cell Cardiol: 57:13-22; 2013 Jan; doi: 10.1016/j.yjmcc.2013.01.001

2012

  • A genome-wide survey for prion-regulated miRNAs associated with cholesterol homeostasis; Montag J, Brameier M, Schmädicke AC, Gilch S, Schätzl HM, Motzkus D; BMC Genomics: 13:486; 2012 Sep; doi: 10.1186/1471-2164-13-486 

2011

  • Unequal allelic expression of wild-type and mutated beta-myosin in familial hypertrophic cardiomyopathy; Tripathi S, Schultz I, Becker E, Montag J, Borchert B, Francino A, Navarro-Lopez F, Perrot A, Özcelik C, Osterziel KJ, McKenna WJ, Brenner B, Kraft T; Basic Res Cardiol; 106(6):1041-55; 2011 Nov; doi: 10.1007/s00395-011-0205-9
  • Bovine spongiform encephalopathy infection alters endogenous retrovirus expression in distinct brain regions of cynomolgus macaques (Macaca fascicularis), Greenwood AD, Vincendeau M, Schmädicke AC, Montag J, Seifarth W, Motzkus D. Mol Neurodegener: 6(1):44; 2011 Jun; doi: 10.1186/1750-1326-6-44

2009

  • Upregulation of miRNA hsa-miR-342-3p in experimental and idiopathic prion disease; Montag J, Hitt R, Opitz L, Schulz-Schaeffer WJ, Hunsmann G, Motzkus D; Mol Neurodegener:4:36, 2009 Aug; doi: 10.1186/1750-1326-4-36

 

Veröffentlichte Abstracts / Preise

2019

  • Contractile imbalance as trigger for HCM pathogenesis: evidence from mutations in different sarcomeric proteins; Montag J, Burkart V, Beck J, Aldag-Niebling D, Piep B, Keyser B, des Remedios C, Hilfiker-Kleiner D, van der Velden J, Kraft T; Eur Heart J:40 ( Supp); 2019
  • Neonatal pigs with a Hypertrophic Cardiomyopathy-mutation knock-in show mild HCM pathology; Montag J, Petersen B, Flögel AK, Becker E, Lucas-Hahn A, Cost GJ, Mühlfeld C, Kraft T, Niemann H, Brenner B; Clin Res Cardiol 108, Suppl 1, V1396; 2019
  • Variable expression of myosin-binding protein C resulting in contractile imbalance from cell-to-cell as possible pathogenic trigger in Hypertrophic Cardiomyopathy; Aldag-Niebling D, Radocaj A, Montag J, Piep B, Keyser B, dos Remedios C, Hilfiker-Kleiner D, Brenner B, Kraft T; Clin Res Cardiol 108, Suppl 1, V1172; 2019

2018

  • Montag J, Petersen B, Flögel AK, Becker E, Lucas-Hahn A, Cost GJ, Mühlfeld C, Kraft T, Niemann H, Brenner B; A Genome Edited Pig with the Hypertrophic Cardiomyopathy-Mutation R723G in the MYH7-gene. Biophysical Journal, Vol. 114, Issue 3, p84a; 2018

2017

  • Knock in of the orthologous hypertrophic cardiomyopathy point mutation R723G in the MYH7 gene of pigs; Montag J, Petersen B, Flögel AK, Becker E, Lucas-Hahn A, Cost GJ, Mühlfeld C, Kraft T, Niemann H, Brenner B; Acta Physiol. 219(S711), p50; 2017
  • Independent, stochastic, burst-like transcription of mutant and wildtype MYH7-alleles. A mechanism for functional and transcriptional variability among HCM (hypertrophic cardiomyopathy) cardiomyocytes; Radocaj A, Kowalski K, Montag J, Kraft T, Brenner B; Acta Physiol. 219(S711):132; 2017
  • Independent Stochastic Burst-Like Transcription of Mutant and Wildtype Alleles as Mechanism for Cell-to-Cell Functional Imbalance in Hypertrophic Cardiomyopathy; Radocaj A, Kowalski K, Montag J, Kraft T, Brenner B; Biophysical Journal, Vol. 112, Issue 3, p164a; 2017
  • A porcine model for the ß-myosin mutation R723G suggests onset of hypertrophic cardiomyopathy during fetal development; Montag J, Petersen B, Flögel AK, Becker E, Lucas-Hahn A, Cost GJ, Mühlfeld C, Kraft T, Niemann H, Brenner B; J Muscle Res Cell Motil 38:391; 2017
  • Intrinsic MYH7 expression regulation may contribute to tissue level allelic imbalance in hypertrophic cardiomyopathy; Montag J, Syring M, Rose J, Weber AL, Ernstberger P, Mayer AK, Becker E, Keyser B, Dos Remedios C, Perrot A, van der Velden J, Francino A, Navarro-Lopez F, Ho CY, Brenner B, Kraft T; J Muscle Res Cell Motil 38:359; 2017
  • Model of independent stochastic burst-like transcription can explain observed functional and transcriptional variability among cardiomyocytes from Hypertrophic Cardiomyopathy patients; Radocaj A, Kowalski K, Montag J, Kraft T, Brenner B; J Muscle Res Cell Motil 38:362; 2017

2016

  • Functional imbalance among individual cardiomyocytes caused by cell-to-cell variation in mutant mRNA expression. Random burst-like transcription as a possible trigger for Hypertrophic Cardiomyopathy;  Kraft T, Montag J, Makul M, Ernstberger P, Tripathi S, Dunda SE, Perrot A, Francino A, Navarro-Lopéz F, Brenner B; Acta Physiol. 216(S707), p58; 2016
  • Kraft T, Makul M, Beck J, Montag J, Radocaj A, Perrot A, Francino A, Navarro-Lopéz F, dos Remedios C, Brenner B; Functional Imbalance among Individual Cardiomyocytes Caused by Cell-to-Cell Variation in Mutant mRNA Expression. A Possible Trigger for Hypertrophic Cardiomyopathy; Biophysical Journal, Vol. 110, Issue 3, p366a; 2016

2015

  • Hypertrophic cardiomyopathy: unequal abundance of mutated myosin in individual cardiomyocytes causing imbalanced force generation—a possible trigger for disease development; Montag J, Makul M, Tripathi S, Dunda SE, Perrot A, Francino A, Navarro-Lopéz F, van der Velden J, Brenner B, Kraft T; J Muscle Res Cell Motil 35:89; 2015
  • Allelic imbalance among individual cardiomyocytes in β-myosin mutation-related hypertrophic cardiomyopathy: a possible trigger for disease development; Kraft T, Makul M, Beck J, Montag J, Tripathi S, Dunda SE, Perrot A, Francino A, Navarro-Lopéz F, Brenner B; Acta Physiol. 213, p15; 2015
  • Myosin mutations in familial hypertrophic cardiomyopathy: functional imbalance among individual cardiomyocytes as trigger for development of myocyte disarray and fibrosis; Beck J, Makul M, Montag J, Ernstberger P, Perrot A, Francino A, Navarro-Lopéz F, Brenner B, Kraft T; Acta Physiol. 213, p184; 2015

2014

  • The ß-myosin mutation R453C in Familial Hypertrophic Cardiomyopathy leads to altered crossbridge kinetics; Montag J, Kraft T, Rose J, List D, McKenna WJ, Brenner B J Muscle Res Cell Motil 35:85; 2014
  • Kraft T, Montag J, Rose J, List D, McKenna WJ, Brenner B; Functional Effects of the β-Myosin Mutation Arg453Cys in Familial Hypertrophic Cardiomyopathy; Biophysical Journal, Vol. 106, Issue 2, p771a; 2014
  • Familial Hypertrophic Cardiomyopathy: Unequal Expression of Mutant and Wildtype Myosin in Individual Myocytes as Trigger for Functional Impairment of the Heart?; Montag J, Tripathi S, Köhler J, Dunda SE, Seebohm B, List D, Matinmehr F, Perrot A, Özcelik C, Francino A, Navarro-Lopéz F, McKenna WJ, van der Velden J, Brenner B, Kraft T; Biophysical Journal, Vol. 106, Issue 2, p644a–645a; 2014
  • How can diverging functional effects of myosin mutations in FHC induce a similar disease phenotype?; Kraft T, Tripathi S, Montag J, Dunda SE, List D, Köhler S, Seebohm B, Matinmehr F, Francino A, Navarro-Lopéz F, McKenna WJ, Perrot A, Osterziel AJ, Özcelik C, Brenner B.; J Muscle Res Cell Motil 35:125, 2014
  • Increased tension cost in hypertrophic cardiomyopathy depends on the location of the mutation in the gene encoding myosin heavy chain; Witjas-Paalberends ER, Montag J, Michels M, ten Cate F, Ho C, Stienen GJM, Poggesi C, Kraft T, van der Velden J.; J Muscle Res Cell Motil 35:131; 2014
  • Montag J, Tripathi S, Köhler J, Dunda S, Seebohm B, List D, Matinmehr F, Francino A, Navarro-Lopéz F, van der Velden J, Brenner B, Kraft T; Cell-to-cell allelic imbalance of mutated vs. wildtype ß-myosin heavy chain expression in Familial Hypertrophic Cardiomyopathy-cardiomyocytes; Acta Physiol. 210(S695), p77; 2014

2012

  • MYH7-mutation associated allelic imbalance in familial hypertrophic cardiomyopathy: Molecular mechanism and correlation with disease prognosis; Montag J, Tripathi S, Weber AL, Schultz I, Becker E, Borchert B, Francino A, Navarro-Lopez F, Perrot A, Özcelik C, Osterziel KJ, McKenna WJ, Brenner B, Kraft T; Journal: Biophysical Journal, Vol. 102, Issue 3, p613a; 2012

2011

  • Mutation-induced allelic imbalance of MYH7: A novel pathogenetic factor in FHC?; Montag J, Tripathi S, Schultz I, Becker E, Borchert B, Francino A, Navarro-Lopez F, Perrot A, Özcelik C, Osterziel KJ, McKenna WJ, Brenner B, Kraft T; J Muscle Res Cell Motil 32:338; 2011

 

Eingeworbene Drittmittel

2011 – 2012

  • HiLF-Programm der MHH: "Expressionsanalyse mutierter b-Myosin-mRNA in laser-mikrodissektierten Kardiomyozyten aus Patienten mit Familiärer Hypertropher Kardiomyopathie": 16.500 €

2011 – 2014

  • Teilprojekt im Arbeitsprogramm "Development of New and Improved Tools to Measure Infection and Disease in Nonhuman Primates" im Verbundantrag EUPRIM-Net II im 7. Forschungsrahmenprogramm der EU: 235.280 €

2019

  • Habilitätsförderung durch das Ellen-Schmidt Programm der MHH

 

Persönliche Förderungen / Preise

2017

  • Stipendium der Gesellschaft der Freunde der MHH für die Teilnahme an der Fortbildung Professional Management: 7000 €