AG Bohne

“RNA rules” (John S. Mattick)

Research summary

Cellular complexity arises from regulation and does not correlate with the number of genes.

The lab focuses on the regulation of gene expression and RNA biology. In terms of viruses we dissect the regulation of herpesviral RNA expression. On the cellular side we are interested in non-coding mutations causing various human diseases. Both areas follow our central idea: A deeper knowledge of both viral gene expression and the pathomechanisms of non-coding mutations will advance our understanding of cellular processes.

AG Bohne/MHH

Regulation of herpesviral gene expression

AG Bohne/MHH

Pathomechanisms of non-coding mutations

AG Bohne/MHH

Retro/lenti viral vector technology

Methods:

The lab uses the classical repertoire of molecular biology techniques. In addition, we focus on RNA analysis using radioactive northern blots for detection of mRNA and miRNAs, RT-qPCR, EMSA and RNA structure analysis. Our toolkit also comprises retro/lentiviral vectors and new technolgies such as CRISPR etc.

Dr. Jens Bohne/MHH
Dr. Jens Bohne

Group members:

Principal investigator

  • PD Dr. Jens Bohne

Medizinische Hochschule Hannover (Hannover Medical School)
Institute of Virology, OE5230
Carl-Neuberg-Str. 1
30625 Hannover

Phone +49 511 532 4307
Departmental Secretary +49 511 532 6736
Laboratory +49 511 532 4319 / 4315

Email Bohne.Jens@MH-Hannover.de


Dr. Carolin Vogt

Postdoc, herpesviral gene expression
+49 511 532 4307 | Vogt.Carolin@MH-Hannover.de

Simon Krooss/MHH

Simon A. Krooss

PhD student, hereditary liver diseases and non-coding mutations
+49 511 532 4292 | Krooss.Simon@MH-Hannover.de

Amelie S. Wachs

PhD student, suppression of 3' end processing
+49 511 532 6493 | Schloesser.Amelia@MH-Hannover.de

Carolina Kuge

Research assistent, HIV restriction and retroviral vectors
+49 511 532 6493 | Kuge.Carolina@MH-Hannover.de

Publications in peer-reviewed journals, Reviews, Book chapters, Comments etc.

Selected Publications:

1. Mendez AS, Vogt C, Bohne J, Glaunsinger BA. Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX. Nucleic Acid Research. 2018 Dec 14; 46(22): 11968-11979.

2. Vogt C and Bohne J. The KSHV RNA regulator ORF57: target specificity and its role in the viral life cycle. Wiley Interdiscip Rev RNA. 2016 Mar-Apr;7(2):173-85.

3. Ripperger T, Manukjan G, Meyer J, Wolter S, Schambach A, Bohne J, Modlich U, Li Z, Skawran B, Schlegelberger B, Steinemann D. The heteromerick transcription factor GABP activates the ITGAM/CD11b promoter and induces myeloid differentiation. Biochim Biophys Acta. 2015 Sep;1849(9):1145-54.

4. Vogt C, Hackmann C, Rabner A, Koste L, Santag S, Kati S, Mandel-Gutfreund Y, Schulz TF, Bohne J. ORF57 overcomes the detrimental sequence bias of Kaposi’s sarcoma-associated herpesvirus lytic genes. J Virol. 2015 May ;89(9):5097-109.

5. Murugan D, Albert MH, Langemeier J, Bohne J, Puchalka J, Järvinen PM, Hauck F, Klenk AK, Prell C, Schatz S, Diestelhorst J, Sciskala B, Kohistani N, Belohradsky BH, Müller S, Kirchner T, Walter MR, Bufler P, Muise AM, Snapper SB, Koletzko S, Klein C, Kotlarz D. Very early onset inflammatory bowl disease associated with aberrant trafficking of IL-10R1 and cure by T cell replete haploidentical bone marrow transplantation. J Clin Immunol. 2014 Apr;34(3):331-9.

6. Schrom EM, Moschall R, Hartl MJ, Weitner H, Fecher D, Langemeier J, Bohne J, Wöhrl BM, Bodem J. U1snRNP-mediated suppression of polyadenylation in conjunction with the RNA structure controls poly (A) site selection in foamy viruses. Retrovirology. 2013 May 29;10:55.

7. Zietara N, Lyszkiewicz M, Witzlau K, Naumann R, Hurwitz R, Langemeier J, Bohne J, Sandrock I, Ballmaier M, Weiss S, Prinz I, Krüger A. Critical role for miR-181a/b-1 in agonist selection of invariant natural killer T cells. Proc Natl Acad Sci. 2013 Apr 30;110(18):7407-12.

8. Langemeier J, Radtke M, Bohne J. U1 snRNP-mediated poly(A) site suppression: beneficial and deleterious for mRNA fate. RNA Biology 2013 Feb;10(2):180-4.

9. Langemeier J, Schrom EM, Rabner A, Radtke M, Zychlinski D, Saborowski A, Bohn G, Mandel-Gutfreund Y, Bodem J, Klein C, Bohne J. A complex immunodeficiency is based on U1snRNP-mediated poly(A) site suppression. EMBO J 2012 Oct 17;31(20):4035-44.

10. Kotlarz D,…. Puchalka J, Bohne J, Egritas O …Grimbacher B, Sauer M, Sykora KW, Koletzko S, Klein C Loss of interleukin-10 signaling and infantile inflammatory bowel disease: implications for diagnosis and therapy. Gastroenterology. 2012 Aug; 143(2):347-55.

Lab Life

AG Bohne/MHH

Auf dem Sambesi im Zoo Hannover (Sambesi river Hannover Zoo) 2019

AG Bohne/MHH

Paddeln auf der Aller (canoeing on the Aller river) 2018

AG Bohne/MHH

Hannover Medical School team run 2018

AG Bohne/MHH

Hiking in the Harz mountains 2017