Scientific Publications



Kempf H, Olmer R, Haase A, Franke A, Bolesani E, Schwanke K, Robles-Diaz D, Coffee M, Göhring G, Dräger G, Pötz O, Joos TO, Martinez-Hackert E, Haverich A, Buettner FF, Martin U, Zweigerdt R. (2016) Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. Nat Communications. 10.1038/ncomms13602


Kropp C, Kempf H, Halloin C, Robles-Diaz D, Franke A, Scheper T, Kinast K, Knorpp T, Joos TO, Haverich A, Martin U, Zweigerdt R, Olmer R. (2016) Impact of Feeding Strategies on the Scalable Expansion of Human Pluripotent Stem Cells in Single-Use Stirred Tank Bioreactors. Stem Cells Transl Med. 10.5966/sctm.2015-0253


Reinisch A, Thomas D, Corces MR, Zhang X, Gratzinger D, Hong WJ, Schallmoser K, Strunk D, Majeti R. (2016) A humanized bone marrow ossicle xentransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells. Nature Medicine. 10.1038/nm.4103




Giacomelli E, Bellin M, Sala L, van Meer BJ, Tertoolen LG, Orlova VV, Mummery CL. (2017). Three-dimensional cardiac microtissues composed of cardiomyocytes and endothelial cells co-differentiated from human pluripotent stem cells. Development. 10.1242/dev.143438


Giacomelli, E., Bellin, M., Orlova, V.V., Mummery, C.L. (2017). Co-differentiation of Human Pluripotent Stem Cells-Derived Cardiomyocytes and Endothelial Cells from Cardiac Mesoderm Provides a Three-dimensional Model of Cardiac Microtissue. Current Protocols in Human Genetics. Curr Protoc Hum Genet. 10.1002/cphg.46.


Guadix, J.A., Orlova, V.V., Giacomelli, E., Bellin, M., Ribeiro, M.C., Mummery, C.L., Pérez-Pomares, J.M., Robert Passier (2017). Human pluripotent stem cell differentiation into functional epicardial progenitor cells. Stem Cell Reports. 10.1016/j.stemcr.2017.10.023.


Jara-Avaca, M., Kempf, H., Ruckert, M., Robles-Diaz, D., Franke, A., de la Roche, J., Fischer, M., Malan, D., Sasse, P., Solodenko, W., Drager, G., Kirschning, A., Martin, U., and Zweigerdt, R. (2017). EBIO Does Not Induce Cardiomyogenesis in Human Pluripotent Stem Cells but Modulates Cardiac Subtype Enrichment by Lineage-Selective Survival. Stem Cell Reports 8 10.1016/j.stemcr.2016.12.012


Konze, S. A., Werneburg, S., Oberbeck, A., Olmer, R., Kempf, H., Jara-Avaca, M., Pich, A., Zweigerdt, R., and Buettner, F. F. (2017). Proteomic Analysis of Human Pluripotent Stem Cell Cardiomyogenesis Revealed Altered Expression of Metabolic Enzymes and PDLIM5 Isoforms. J Proteome Res Res. 10.1021/acs.jproteome.6b00534


Kropp C, Massai D, Zweigerdt R. (2017). Progress and challenges in large-scale expansion of human pluripotent stem cells. Process Biochemistry. 10.1016/j.procbio.2016.09.032


Massai, D., Bolesani, E., Diaz, D. R., Kropp, C., Kempf, H., Halloin, C., Martin, U., Braniste, T., Isu, G., Harms, V., Morbiducci, U., Drager, G., and Zweigerdt, R. (2017). Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: considerations on process development. Sci Rep 7, no. 1 10.1038/s41598-017-04158-x


Pachler, K; Ketterl, N; Desgeorges, A; Dunai, ZA; Laner-Plamberger, S; Streif, D; Strunk, D; Rohde, E; Gimona, M. (2017) An In Vitro Potency Assay for Monitoring the Immunomodulatory Potential of Stromal Cell-Derived Extracellular Vesicles. Int J Mol Sci. 10.3390/ijms18071413


Sheffield NC, Pierron G, Klughammer J, Datlinger P, Schönegger A, Schuster M, Hadler J, Surdez D, Guillemot D, Lapouble E, Freneaux P, Champigneulle J, Bouvier R, Walder D, Ambros IM, Hutter C, Sorz E, Amaral AT, de Álava E, Schallmoser K, Strunk D, Rinner B, Liegl-Atzwanger B, Huppertz B, Leithner A, de Pinieux G, Terrier P, Laurence V, Michon J, Ladenstein R, Holter W, Windhager R, Dirksen U, Ambros PF, Delattre O, Kovar H, Bock C, Tomazou EM. (2017). DNA methylation heterogeneity in Ewing sarcoma defines an epigenetic disease spectrum underlying a genetically homogeneous developmental cancer. Nature  Medicine. 10.1038/nm.4273


Souidi N, Stolk M, Rudeck J, Strunk D, Schallmoser K, Volk HD, Seifert M. (2017) Stromal Cells Act as Guardians for Endothelial Progenitors by Reducing Their Immunogenicity After Co-Transplantation. Stem Cells. 10.1002/stem.2573



Ackermann, M., Kempf, H., Hetzel, M., Hesse, C., Hashtchin, A.R., Brinkert, K., Schott, J.W., HaakemK., Kühnel, M.P., Glage, S., Figueiredo, C., Jongk, D., Sweald, K., Schambach, A., Wronksi, S., Moritz, T., Martin, U., Zweigerdt, R., Munder, A., Lachmann, N.(2018). Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections, Nature Communications 10.1038/s41467-018-07570-7


Allison TF, Andrews PW, Avior Y, Barbaric I, Benvenisty N, Bock C, Brehm J, Brüstle O, Damjanov I, Elefanty A, Felkner D, Gokhale PJ, Halbritter F, Healy LE, Hu TX, Knowles BB, Loring JF, Ludwig TE, Mayberry R, Micallef S, Mohamed JS, Müller FJ, Mummery CL, Nakatsuji N, Ng ES, Oh SKW, O'Shea O, Pera MF, Reubinoff B, Robson P, Rossant J, Schuldt BM, Solter D, Sourris K, Stacey G, Stanley EG, Suemori H, Takahashi K, Yamanaka S. (2018). Assessment of established techniques to determine developmental and malignant potential of human pluripotent stem cells. Nature Communications  10.1038/s41467-018-04011-3


Castilho, M., van Mil, A., Maher, M., Metz, C.H.G., Hochleitner, G., Groll, J., Doevendans, P.A., Ito, K., Sluijter, J.PO.G., Malda, J.(2018). Melt Electrowriting Allows Tailored Microstructural and Mechanical Design of Scaffolds to Advance Functional Human Myocardial Tissue Formation. Advanced Functional Materials 10.1002/adfm.201803151


Christofferson, J., Meier, F., Kempf, H., Schwanke, K., Coffee, M., Beilmann, M., Zweigerdt, R., Mandenius, C.-F.(2018). A Cardiac Cell Outgrowth Assay for Evaluating Drug Compounds Using a Cardiac Spheroid-on-a-Chip Device. Bioengineering 10.3390/bioengineering5020036


Eicke, D., Baigger, A., Schulze K., Latham, S.L., Halloin, C., Zweigerdt, R., Guzman, C.A., Blasczyk, R., Figueiredo, C.(2018). Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors. Scientific Reports 10.1038/s41598-018-28459-x


Froese, N., Wang, H., Zwadlo, C., Wang, Y., Grund, A., Gigina, A., Hofmann, M., Kilian, K., Scharf, G., Korf-Klingebiel, M., Melchert, A., Ricci Signorini, M.E., Halloin, C., Zweigerdt, R., Martin, U., Gruh, I., Wollert, K.C., Geffer, R., Bauersachs, J., Heineke, J. (2018). Anti-androgenic therapy with finasteride improves cardiac function, attenuates remodeling and reverts pathologic gene-expression after myocardial infarction in mice. Journal of Molecular and Cellular Cardiology 10.1016/j.yjmcc.2018.08.011


Gaspari, E., Franke, A., Robles-Diaz, D., Zweigerdt, R., Roeder, I., Zerjatke, T., Kempf, H.(2018). Paracrine mechanisms in early differentiation of human pluripotent stem cells: Insights from a mathematical model. Stem Cell Research 10.1016/j.scr.2018.07.025


Gieseler, G.-M., Ekramzadeh K., Nölle, V., Malysheva, S., Kempf, H., Beutel, S., Zweigerdt, R., Martin, U., Rinas, U., Scheper, T., Pepelanova, I. (2018) Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies. Biotechnology Reports 10.1016/j.btre.2018.e00249


Iorga, B., Schwanke, K., Weber, N., Wendland, M., Greten, S., Piep, B., Dos Remedios, C.G., Martin, U., Kraft, T., Brenner, B.(2018). Differences in Contractile Function of Myofibrils within Human Embryonic Stem Cell-Derived Cardiomyocytes vs. Adult Ventricular Myofibrils Are Related to Distinct Sarcomeric Protein Isoforms, Frontiers in Physiology 10.3389/fphys.2017.01111


Halaidych, O., Freund, C., van den Hil, F., Salvatori, D.C.F., Riminucci, M., Mummery, C.L., Orlova, V.V. (2018). Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells. Stem Cell Reports 2018; 10.1016/j.stemcr.2018.03.012


Hochmann, S., Mittermeir, M., Santic, R., Koszik, F., Griessner, L., Sonderegger, A.S., Hoffmann, T., Russe, E., Scheiblhofer, S., Weiss, R., Mandler, M., Schneeberger, A., Strunk, D. (2018). Evaluation of modified Interferon alpha mRNA constructs for the treatment of non-melanoma skin cancer. Scientific Reports 10.1038/s41598-018-31061-w


Kempf, H., Zweigerdt, R.(2018). Scalable Cardiac Differentiation of Pluripotent Stem Cells Using Specific Growth Factors and Small Molecules. Engineering and Application of Pluripotent Stem Cells 10.1007/10_2017_30


Kyriakides, O., Halliwell, J., Andrews, P.(2018). Engineering and applications of pluripotent stem cells - Acquired genetic and epigenetic variation in human pluripotent stem cells Adv Biochem Eng Biotechnol. 10.1007/10_2017_22.(Book chapter)


Lipps C., Klein, F., Wahlicht, T., Seiffert, V., Butueva, M., Zauers, J., Truschel, T., Luckner, M., Köster, M., MacLeod, R., Pezoldt, J., Hühn, J., yuan, Q., Müller, P.P., Kempf, H., Zweigerdt, R., Dittrich-Breiholz, O., Pufe, T., Beckmann, R., Drescher, W., Riancho, J., Sañudo, C., Korff, T., Opalka, B., Rebmann, V., Göthert, J., Alves, P.M., Ott, M., Schucht, R., Hauser, H., Wirth, D., May, T. (2018). Expansion of functional personalized cells with specific transgene combinations. Nature Communications 10.1038/s41467-018-03408-4


Loch L., Deiwick, A., Franke, A., Schwanke, K., Haverich, A., Zweigerdt, R., Chichkov, B.(2018). Laser bioprinting of human induced pluripotent stem cells—the effect of printing and biomaterials on cell survival, pluripotency, and differentiation. Biofabrication 2018; 10.1088/1758-5090/aab981


Oeller, M., Laner-Plamberger, S., Hochmann, S., Ketterl, N., Feichtner, M., Brachtl, G., Hochreiter, A., Scharler, C., Bieler, L., Romanelli, P., Couillard-Despres, S., Russe, E., Schallmoser, K., Strunk, D. (2018). Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal Cells. Theranostics 10.7150/thno.21906


Olmer, R., Engels, R., Usman, A., Menke, S., Hayat Malik, M.N., Pessler, F., Göhring, G., Bornhorst, D., Bolten, S., Abdelilah-Seyfried, S., Scheper, T., Kempf, H., Zweigerdt, R., Martin, U.(2018).Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture, Stem Cell Reports 10.1016/j.stemcr.2018.03.017


Weist, R., Flörkemeier, T., Roger, Y., Noack, S., Franke, A., Schwanke, K., Zweigerdt, R., Martin, U., Willbold, E., Hoffmann, A.(2018). Differential Expression of Cholinergic System Components in Human Induced Pluripotent Stem Cells, Bone Marrow-Derived Multipotent Stromal Cells, and Induced Pluripotent Stem Cell-Derived Multipotent Stromal Cells. Stem Cells and Development 10.1089/scd.2017.0162


Wolling, H., Konze, S.A., Höfer, A., Erdmann, J., Pich, A., Zweigerdt, R., Buettner, F.F.R. (2018). Quantitative Secretomics Reveals Extrinsic Signals Involved in Human Pluripotent Stem Cell Cardiomyogenesis, Proteomics 10.1002/pmic.201800102


Van Mil, A., Balk, G.M., Neef, K., Buikema, J.W., Asselbergs, F.W., Wu, S.M., Doevendans, P.A., Sluijter, J.P.G. (2018).Modelling inherited cardiac disease using human induced pluripotent stem cell-derived cardiomyocytes: progress, pitfalls, and potential. Cardiovascular Research 10.1093/cvr/cvy208



Madonna, R., Van Laake, L.W., Botker, H.E., Davidson, S.M., De Caterina, R., Engel, F.B., Eschenhagen, T., Fernandez-Avilez, F., Hausenloy, D.J., Hulot, J.-S., Lecour, S., Leor, J., Menasche, P., Pesce, M., Perrino, C., Prunier, F., Van Linthout, S., Ytrehus, K., Zimmermann, W.-H., Ferdinandy, P., Sluijter, J.P.G. (2019) ESC Working Group on Cellular Biology of the Heart: position paper for Cardiovascular Research: tissue engineering strategies combined with cell therapies for cardiac repair in ischaemic heart disease and heart failure. Cardiovascular Research 10.1093/cvr/cvz010


Conference Contributions


Posters and Presentations



Introducing an animal serum-free potency assay to monitor immune modulation capacity of human mesenchymal stem/progenitor cells (MSPCs), Nina Ketterl, PMU, June and October 2016, PMU Science Get Together 2016, Salzburg, Austria and ESGCT and ISSCR Collaborative Congress, Florence, Italy

Stepwise maturation of human iPS cells into immunosuppressive mesenchymal stem/progenitor cells Cornelia Scharler, PMU, September 2016 and October 2016, 4th Annual Meeting of the German Stem Cell Network (GSCN), Hannover, Germany; 3rd PACT-Symposium: Designer Cells Go Clinic, Vienna, Austria and ESGCT and ISSCR Collaborative Congress, Florence, Italy

Assessment of μ-Tissue in Porcine Myocardial Infarction Model for Cell-Based Heart RepairEvelyne Demkes, UMCU, October 2016, Dutch Heart Foundation Phd course: Vascular Biology; Papendal, the Netherlands

Scalable Expansion of hMSCs in BioBLU Single-Use Vessels, Ulrike Becken, Stefan Wilson, DASGIP; September 2017, Mesenchymal Stem Cell Meeting, Chester, UK

Scalable Expansion of hMSCs in BioBLU Single-Use Vessels, Ulrike Becken, Aurelie Tacheny, DASGIP, October 2017, Bone-Tec October, October 2017, Munich, Germany


Adaptation of Cell Culture Methods for the Expansion of Mesenchymal Stem Cells in Stirred-Tank Bioreactors, Ulrike Becken, Vincent Dufey, DASGIP & Eppendorf, January 2018, ESACT UK, Leeds, UK

Scalable Production of hiPSC-derived Cardiomyocyes in Stirred-Tank Bioreactors P. Nold, Ulrike Becken, DASGIP, April 2018, 2nd Stem Cell Community Day Düsseldorf, Germany

Scalable Production of hiPSC-derived Cardiomyocyes in Stirred-Tank Bioreactors,  P. Nold, Ulrike Becken, DASGIP, May 2018, ISCT, Montreal, Canada


Scalable Production of hiPSC-derived Cardiomyocyes in Stirred-Tank Bioreactors,  P. Nold, Ulrike Becken, DASGIP, June  2018, ISSCR, Melbourne, Austria

Adaptation of Cell Culture Methods for the Expansion of Mesenchymal Stem Cells in Stirred-Tank Bioreactors, Ulrike Becken, Marc Skoupi, DASGIP, September 2018, ESBES, Lisbon, Portugal


Presentations and Talks


Cardiac derivatives of hPSC: from development tobeating heart,Christine Mummery, LUMC, March 2017, Keystone Conference, Keystone USA

Genetic Changes in Human Pluripotent Stem Cells: Implications for Regenerative Medicine, Ivana Barbaric, USF, February 2017, PluriMes Third Annual Consortium Meeting, Laax, Switzerland

Differentiation and Functionality of Endothelial Cells, Pericytes and vSMCs from Human Induced Pluripotent Stem Cells, Valeria Orlova, LUMC, September 2016, 5th Utrecht Stem Cell Conference 2016, Utrecht, The Netherlands


Stepwise maturation of human iPS cells into immunosuppressive mesenchymal stem/progenitor cells Cornelia Scharler, PMU, September and October 2016, 4th Annual Meeting of the German Stem Cell Network (GSCN), Hannover, Germany and 3rd Research Conference of the Spinal Cord Injury & Tissue Regeneration Center Salzburg (SCI-TReCS), Salzburg, Austria


Genetic stability of human pluripotent stem cells, Ivana Barbaric, Peter Andrews, USF, October 2016, Webinar hosted by Stem Cell Technologies

Introduction to ISCI Workshop: Origins and Implications of Pluripotent Stem Cell Genetic and Epigenetic Instability: 'Defining the problem', Peter Andrews, USF, October 2016, ISCI Workshop: Origins and Implications of Pluripotent Stem Cell Genetic and Epigenetic Instability, Bar Harbor, USA

Detection of genetic mosaicism in human pluripotent stem cell cultures, Ivana Barbaric, USF, October 2016; ISCI Workshop: Origins and Implications of Pluripotent Stem Cell Genetic and Epigenetic Instability, Bar Harbor, USA

Development of immunosuppressive iPSC-derived stromal cells for tissue generation, Cornelia Scharler, Patrizia Peking, Nina Ketterl, Katharina Schallmoser, Dirk Strunk, PMU; September 2018, 6th Annual Meeting of the German Stem Cell Network (GSCN), Hannover, Germany


Short presentations of current projects including TECHNOBEAT at university departments and companies on the Innovation Journey Kyoto - Osaka - Tokyo:

10/12/2018: Center for iPS Cell Research and Application (CIRA), Kyoto University;
10/12/2018: Megakaryon Corp., Kyoto; 
11/12/2018: Osaka Bio Headquarters, Life Science Cluster Osaka;
11/12/2018: Matrixome Inc., Regenerative Medicine, Osaka;
11/12/2018: Sumitomo Dainippon Pharma, Osaka;
12/12/2018: Japan Agency for Medical Research and Development (AMED), Tokyo;
12/12/2018: Healios, Tokyo;
12/12/2018: Keio University, Okano Laboratories, Tokyo;
13/12/2018: Shonan Health Innovation Park, Tokyo.
Katharina Schallmoser, Dirk Strunk, PMU; Innovation journey on "Stem Cell Therapy - from Bench to Bedside" (Kyoto - Osaka - Tokyo) of the Austrian Embassy, Commercial Section, Tokyo, Japan


Christine Mummery, Valeria Orlova, Elisa Giacomelli, LUMC 2017-2018 various talks on the topics of Cardiomyocytes from human pluripotent stem cells and Vascular cells from human pluripotent stem cells. Click here for list of events.


Challenges in Stem Cell Production, Ulrike Becken & Eppendorf, Christof Knocke DASGIP & Eppendorf, 5th German Stem Cell Network conference, September 2017, Jena, Germany

Scalable Stem Cell Cultivation in Stirred-Tank Bioreactors, Ulrike Becken, Sandra Beyer, DASGIP & Eppendorf, February 2018, Charleroi, Belgium


DASGIP/Eppendorf hosted the 2nd Stem Cell Community Day, Chair Prof. Joaquim M. S. Cabral April 2018, venue: Düsseldorf, Germany

Stem Cell Expansion in Stirred-Tank Bioreactors, Philip Nold, DASGIP, May 2018, BACT, Frankfurt, Germany

Stem Cell Bioprocessing: Process Design, Scale-up, and Product Safety, Philip Nold, DASGIP, September 2018, 6th Annual German Stem Cell Network Meeting, Heidelberg, Germany


Stem Cell Cultivation in BioBLU Single-Use Vessels, Ulrike becken, Philip Nold, DASGIP, October 2018, AMC 17th Technical Meeting October 2018, Bristol, UK


Stem Cell Cultivation in BioBLU Single-Use Vessels
Stem Cell Bioprocessing: Process Design, Scale-Up, and Product Safety, Lay Koon Goh, Eppendorf, October 2018, Stem Cell Day Asia Seoul, Korea


iPSC genomic instability and impact on product safety, Peter Andrews, February 2018, "Safety assessment of cell therapy products: current advance challenges"  Cell and Gene Therapy Catapult, London UK


Pluripotency tests, Peter Andrews, September 2018, "Round Table: Challenges in ensuring hPSC quality", Nature, London, UK


Culture acquired genetic variants of human pluripotent stem cells, Peter Andrews, November 2018, "4th Meeting of the Germ Stem Cell Cores", German Stem Cell Network, Charité Universitätsmedizin Berlin, Germany


Genomic aberrations in human embryonic stem cells, Ivana Barbaric, July 2017, 33rd Annual Meeting European Society of Human Reproduction and Embryology, Geneva, Switzerland


Genetic changes in human pluripotent stem cells: implications for regenerative medicine, Ivana Barbaric, February 2018, "Human Pluripotent Stem Cells and Regenerative Medicine: The Here and Now and Future of our Field"; Francis Crick Institute, London, UK


Detecting, defining and understanding functional significance of (epi)genetic variants, Ivana Barbaric, May 2018, The Pluripotent and Engineered Stem Cell Platform kick-off meeting, London, UK


Culture-acquired genetic changes in human pluripotent stem cells, Ivana Barbaric, September 2018, "Round Table: Challenges in ensuring hPSC quality", Nature, London, UK


Culture-acquired genetic changes in human pluripotent stem cells, Ivana Barbaric, October 2018, Wellcome Collection, London, UK

Patching up damaged hearts, Joost Sluijter, UMCU, October 2016, Connecting U symposium "Engineered tissues & organs", Utrecht, the Netherlands

Targeting myocardial repair, Joost Sluijter, UMCU, October 2016, Gangzhou University, Gangzhou, China.


Public Outreach


Open days, Exhibitions, Schools etc.


MRC festival of medical research, Jason Halliwell, USF, June 2016, Winter Gardens, Sheffield, U.K.


Pleisters voor het hart (lecture to highschool students), Joost Sluijter, UMCU, February 2017, Middelbare school Parelhoen, Amersfoort, The Netherlands


The power of stem cells, Ivana Barbaric, USF, September 2018, Soapbox Science outreach event, Sheffield UK


Articles in industry newletters, journals etc.


Collaboration is key
Medicine Maker/Stephanie Sutton, Katharina Kinast, Ulrike Becken, Robert Zweigerdt
The Medicine Maker’s Advancing Medicine supplement (print)

Listen to the Heartbeat
Katharina Kinast, Ulrike Becken, Robert Zweigerdt

Medicine Maker/Stephanie Sutton

Editorial "Controlled Stem Cell Production"
Ulrike Becken/ DASGIP
Eppendorf Customer Magazine BioNews, H2/2017

"Internationale Experten beim zweiten Stem Cell Community Day"
LABO 7-8/2018

A question of consistency: biopharma know-how meets bioreactor innovation in upstream bioprocessing
Cell gene Therapy Insights & DASGIP, Philip Nold

Cell Gene Therapy Insights
Expert View: Large-scale stem cell expansion and differentiation
Drug Target Review & DASGIP, Philip Nold