The major aim of our laboratory is to develop molecular medicine approaches for the treatment of liver disease. To achieve this, we are utilizing interdisciplinary approaches involving cell culture disease modeling, mouse experiments and biomaterials from clinical samples. Over the years, our work has uncovered the functional relevance of small RNAs and growth factors during progression of acute and chronic liver diseases. Additionally, we have discovered that transcription-factor induced reprogramming in hepatic cells holds a great potential for the attenuation of liver fibrosis and cirrhosis.
We are currently investigating the mechanisms underlying cell fate conversion of hepatic cells during reprogramming. A second focus of our group is to develop RNA therapeutics for amelioration of liver diseases. We are investigating the therapeutic potential of various types of RNAs such as viral RNA and messenger RNA in mouse models of liver diseases.
- DFG, REBIRTH Research Center for Translational and Regenerative Medicine
- Plus 3 Program of Boehringer Ingelheim
- Funding from biopharmaceutical companies
PD Dr. Amar Deep Sharma
Medizinische Hochschule Hannover OE 6810
Carl-Neuberg-Str. 1
30625 Hannover
Telefon +49 511 532-5255
Unsere Mitarbeiterinnen und Mitarbeiter
In den folgenden Aufklappksästen finden Sie eine Übersicht unserer Mitarbeiterinnen und Mirarbeiter. Bei weiteren Fragen zu unserem Team können Sie sich jederzeit an uns wenden.
Medizinische Hochschule Hannover OE 6810
Carl-Neuberg-Str. 1
30625 Hannover
Telefon +49 511 532-5255
- Antonia Hapke (BTA)
- Zhen Dai (Postdoc)
- Jovana Markovic (PhD student)
- Taihua Yang (PhD student)
- Alexandra Bogomolova (PhD student)
- Ruomeng Li (PhD student)
- Guangqi Song (PhD student: currently working as a Group leader, at Shanghai Institute of Liver diseases, China)
- Selina Möbus (PhD student and later postdoc: currently working as scientist at Lophius Biosciences Gmbh, Regensburg, Germany)
- Dakai Yang (PhD student and later postdoc: currently working as a postdoc at Stanford, CA, USA)
- Helena Anke (MD student: currently at Hannover Medical School)
- Daniela Vallejo Guerra (Trainee: currently working as a postdoc at Center for Aging and Regeneration Santiago, Chile)
- Annabelle Hartwig (BTA)
Unsere Publikationen
In den folgenden Aufklappksästen finden Sie eine Übersicht unserer Publikationen. Bei weiteren Fragen zu unserer klinischen und wissenschaftlichen Arbeit können Sie sich jederzeit an uns wenden.
- Dai Z, Ott M, Sharma AD. Equal opportunity offer for all hepatocytes. Journal of Hepatology 2020. doi: 10.1016/j.jhep.2020.03.038. [Epub ahead of print].
- Dai Z, Song G, Balakrishnan A, Yang T, Yuan Q, Mobus S, Weiss AC, Bentler M, Zhu J, Jiang X, Shen X, Bantel H, Jaeckel E, Kispert A, Vogel A, Saborowski A, Buning H, Manns M, Cantz T, Ott M, Sharma AD (2020). Growth differentiation factor 11 attenuates liver fibrosis via expansion of liver progenitor cells. Gut. doi: 10.1136/gutjnl-2019-318812. [Epub ahead of print].
- Krooss SA, Dai Z, Schmidt F, Rovai A, Fakhiri J, Dhingra A, Yuan Q, Yang T, Balakrishnan A, Steinbrück L, Srivaratharajan S, Manns MP, Schambach A, Grimm D, Bohne J, Sharma AD, Büning H, Ott M. Ex Vivo/In vivo Gene Editing in Hepatocytes Using "All-in-One" CRISPR-Adeno-Associated Virus Vectors with a Self-Linearizing Repair Template. iScience. 2020 Jan 24;23(1):1000764.
- Song G, Yuan Q, Dai Z, Tsay HC, Shen X, Ott M, Sharma AD. Conversion of fibroblasts to hepatocyte-like cells in vivo. Methods Mol Biol. 2019;1905:103-115.
- Tsay HC, Yuan Q, Balakrishnan A, Kaiser M, Möbus S, Kozodrowska E Farid M, Tegtmeyer PK, Borst K, Vondran FWR, Kalinke U, Kispert A, Manns MP, Ott M, Sharma AD. Hepatocyte-specific suppression of microRNA-221-3p mitigates liver fibrosis. Journal of Hepatology 2018 Dec 21. DOI: 10.1016/j.jhep.2018.12.016. [Epub ahead of print].
- Sgodda M, Dai Z, Zweigerdt R, Sharma AD, Ott M, Cantz T (2017). A scalable approach for the generation of human pluripotent stem cell-derived hepatic organoids with sensitive hepatotoxicity features. Stem Cells Dev. 26(20):1490-1504
- Yang D, Yuan Q, Balakrishnan A, Bantel H, Klusmann JH, Manns MP, Ott M, Cantz T, Sharma AD (2016). MicroRNA-125b-5p mimic inhibits acute liver failure. Nat Commun. 7:11916. doi: 10.1038/ncomms11916.
- Song G, Pacher M, Balakrishnan A, Yuan Q, Tsay H-C, Yang D, Reetz J, Brandes S, Dai Z, Pützer BM, Arauzo-Bravo MJ, Steinemann D, Luedde T, Schwabe RF, Manns MP, Schöler HR, Schambach A, Cantz T, Ott M, Sharma AD (2016). Direct reprogramming of hepatic myofibroblasts into hepatocytes in vivo attenuates liver fibrosis. Cell Stem Cell. 18(6):797-808.
- Möbus S, Yang D, Yuan Q, Lüdtke TH, Balakrishnan A, Sgodda M, Rani B, Kispert A, Vogel A, Manns MP, Ott M, Cantz T, Sharma AD. MicroRNA-199a-5p inhibition enhances liver repopulation ability of human embryonic stem cell-derived hepatic cells. Journal of Hepatology 2015;62:101-10.
- Yuan Q, Loya K, Rani B, Möbus S, Balakrishnan A, Lamle J, Cathomen T, Vogel A, Manns MP, Ott M, Cantz T, Sharma AD. MicroRNA-221 overexpression accelerates hepatocyte proliferation during liver regeneration. Hepatology 2013;57(1):299-310.