• investigation of subcellular GCPR signaling in cell culture, organotypic environment and in living animals
  • Characterization of specific G-proteins and downstream effectors involved in GPCR-mediated signal, cross-talk and feedback mechanisms
  • Post-translational modifications of the signaling proteins and their role in the regulation of the signal transduction

In our experiments we apply all current methods of molecular and cell biology as well as protein biochemistry and receptor pharmacology. In addition, we possess broad experiences in electrophysiology (patch-clamp), time-lapse microscopy and fluorescence microscopy. I our lab we also established the modern biophysical approaches for molecular imaging including, Förster resonance energy transfer (FRET) in cuvette and at single-cell level, fluorescence life-time measurements (FLIM) as well as bioluminescence resonance energy transfer (BRET). To analyse the expression level of different proteins we routinely use the quantitative RT-PCR analysis (TaqMan and SYBR Green) as well as single-cell RT-PCR techniques. 

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