Generation of human induced pluripotent stem cell lines
Encoding for genetically encoded calcium indicators RCaMP1h and GCaMP6f
Calcium plays a key role in cardiomyocytes (CMs) for the translation of the electrical impulse of an action potential into contraction forces. A rapid, not-invasive fluorescence imaging technology allows for the monitoring of calcium transients in human induced pluripotent stem cell derived-cardiomyocytes (hiPSC-CMs) to investigate the cardiac electrophysiology in vitro and after cell transplantation in vivo. The genetically encoded calcium indicators (GECIs) GCaMP6f or RCaMP1h were successfully transfected in the previously established hiPSC line MHHi001-A, together with a cardiac specific antibiotic selection cassette facilitating the monitoring of the calcium handling in highly pure populations of hiPSC-CMs.
- Maria Elena Ricci Signorini (LEBAO, MHH)
- Ina Gruh (LEBAO, MHH)
- Monika Szepes, Anna Melchert, Mine Bakar, Sylvia Merkert, Alexandra Haase, Ulrich Martin (LEBAO, MHH)
- Gudrun Göhring (Department of Human Genetics, MHH)
For more information, contact Prof. Ina Gruh, Gruh.Ina@mh-hannover.de