Bohne lab

“RNA rules” (John S. Mattick)

Research summary

Cellular complexity arises from regulation and does not correlate with the number of genes.

The lab focuses on the regulation of gene expression and RNA biology. In terms of viruses we dissect the regulation of herpesviral RNA expression. On the cellular side we are interested in non-coding mutations causing various human diseases. Both areas follow our central idea: A deeper knowledge of both viral gene expression and the pathomechanisms of non-coding mutations will advance our understanding of cellular processes.

Methods:

The lab uses the classical repertoire of molecular biology techniques. In addition, we focus on RNA analysis using radioactive northern blots for detection of mRNA and miRNAs, RT-qPCR, EMSA and RNA structure analysis. Our toolkit also comprises retro/lentiviral vectors and new technolgies such as CRISPR etc.

Publications in peer-reviewed journals, Reviews, Book chapters, Comments etc.

Ten most recent Publications:

1. Wachs AS, Bohne J. Two sides of the same medal: Noncoding mutations reveal new pathological mechanisms and insights into the regulation of gene expression. Wiley Interdiscip Rev RNA. 2021 Jan;12(1):e1616. Review.

2. Krooss S, Werwitzke S, Kopp J, Rovai A, Varnholt D, Wachs AS, Goyenvalle A, Aarstma-Rus A, Ott M, Tiede A, Langemeier J, Bohne J. Pathological mechanism and antisense oligonucleotide-mediated rescue of a non-coding variant suppressing factor 9 RNA biogenesis leading to hemophilia B. PLoS Genet. 2020 Apr 8;16(4):e1008690.

3. Krooss SA, Dai Z, Schmidt F, Rovai A, Fakhiri J, Dhingra A, Yuan Q, Yang T, Balakrishnan A, Steinbrück L, Srivaratharajan S, Manns MP, Schambach A, Grimm D, Bohne J, Sharma AD, Büning H, Ott M. Ex Vivo/In vivo Gene Editing in Hepatocytes Using “All-in-One” CRISPR-Adeno-Associated Virus Vectors with a Self-Linearizing Repair Template. iScience. 2020 Jan 24;23(1):100764.

4. Illig D, Navratil M, Kelečić J, Conca R, Hojsak I, Jadrešin O, Ćorić M, Vuković J, Rohlfs M, Hollizeck S, Bohne J, Klein C, Kotlarz D. Alternative Splicing Rescues Loss of Common Gamma Chain Function and Results in IL-21R-like Deficiency. J Clin Immunol. 2019 Feb;39(2):207-215.

5. Stalke A, Pfister ED, Baumann U, Eilers M, Schäffer V, Illig T, Auber B, Schlegelberger B, Brackmann R, Prokisch H, Krooss S, Bohne J, Skawran B. Homozygous frame shift variant in ATP7B exon 1 leads to bypass of nonsense-mediated mRNA decay and to a protein capable of copper export. Eur J Hum Genet. 2019 Jun;27(6):879-887.  

6. Mendez AS, Vogt C, Bohne J, Glaunsinger BA. Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX. Nucleic Acid Research. 2018 Dec 14; 46(22): 11968-11979.

7. Vogt C and Bohne J. The KSHV RNA regulator ORF57: target specificity and its role in the viral life cycle. Wiley Interdiscip Rev RNA. 2016 Mar-Apr;7(2):173-85.

8. Ripperger T, Manukjan G, Meyer J, Wolter S, Schambach A, Bohne J, Modlich U, Li Z, Skawran B, Schlegelberger B, Steinemann D. The heteromerick transcription factor GABP activates the ITGAM/CD11b promoter and induces myeloid differentiation. Biochim Biophys Acta. 2015 Sep;1849(9):1145-54.

9. Vogt C, Hackmann C, Rabner A, Koste L, Santag S, Kati S, Mandel-Gutfreund Y, Schulz TF, Bohne J. ORF57 overcomes the detrimental sequence bias of Kaposi’s sarcoma-associated herpesvirus lytic genes. J Virol. 2015 May ;89(9):5097-109.

10. Murugan D, Albert MH, Langemeier J, Bohne J, Puchalka J, Järvinen PM, Hauck F, Klenk AK, Prell C, Schatz S, Diestelhorst J, Sciskala B, Kohistani N, Belohradsky BH, Müller S, Kirchner T, Walter MR, Bufler P, Muise AM, Snapper SB, Koletzko S, Klein C, Kotlarz D. Very early onset inflammatory bowl disease associated with aberrant trafficking of IL-10R1 and cure by T cell replete haploidentical bone marrow transplantation. J Clin Immunol. 2014 Apr;34(3):331-9.