Methods Laser microdissection (Single cell-) Reverse Transcriptase PCR (RT-PCR) single molecule RNA-fluorescence in situ hybridisation (smRNA-FISH) Kontraktions- und Ca2+-Transientenmeasurement with MyoCam

of individual CMs in the different categories of MyHC protein isoform expression as revealed by fluorescent labeling for plated hESC-CMs and hESC-CMs from cardiac bodies (CBs). Figure modified from Weber

A., Mueller, P.P., Warnecke, A., et al (2013) Evaluation of single-cell force spectroscopy and fluorescence microscopy to determine cell interactions with femtosecond-laser microstructured titanium surfaces [...] R., Popilka, L., Marcinowski, L., Taeglich, M., Jung, C., et al (2012) A beta-herpesvirus with fluorescent capsids to study transport in living cells. PLoS One 7: e40585. 126. Brand, F., Schumacher, S. [...] (2007). Nuclear egress and envelopment of Herpes Simplex Virus capsids analyzed with dual-color fluorescence HSV1(17+). J Virol. 235. Nolle, A., Zeug, A., van Bergeijk, J., Tonges, L., Gerhard, R., Brinkmann

Schwanke, K., Zweigerdt, R., Martin, U., Saito, S., Haverich, A., and Kutschka, I. 2014. Macroscopic fluorescence imaging: a novel technique to monitor retention and distribution of injected microspheres in an [...] Schmiedl, A., Groos, S., Haverich, A., and Martin, U. 2008. Type II pneumocyte-restricted green fluorescent protein expression after lentiviral transduction of lung epithelial cells. Hum Gene Ther 19, no [...] Ruhparwar, A., Kofidis, T., Ruebesamen, N., Karck, M., Haverich, A., and Martin, U. 2005. Intra-vital fluorescence microscopy for intra-myocardial graft detection following cell transplantation. Int J Cardiovasc

metabolism of extracellular sphingosine 1-phosphate in vivo and in vitro by imaging and analyzing fluorescently-labelled sphingolipids Supervisor:Dr. Markus Gräler, Institute of Immunology, Hannover Medical