Primary Human Hepatocyte Core Facility The Primary Human Hepatocyte (PHH) Core Facility at the Hannover Medical School (MHH) provides research groups with freshly isolated hepatocytes from human liver tissue
thesis: „Effects of Culture Conditions and Cardiomyogenic Differentiation on the (Glyco-) Proteome of Human Pluripotent Stem Cells“ 2010: diploma thesis at the Diplomarbeit Institute for Clinical Biochemistry [...] imbalance among cardiomyocytes as pathogenic factor for Hypertrophic Cardiomyopathy – investigations on human pluripotent stem-cell derived cardiomyocytes carrying MyBP-C-mutations. The current project focuses [...] future therapeutic approaches. Expertise Scientific expertise: Cultivation and differentiation of human induced pluripotent stem cells (hiPSCs), particularly to cardiomyocytes and skeletal muscle cells
on enables direct PCR screening for footprintless targeted human pluripotent stem cells”, Stem Cell Reports 2014 Genetic engineering of human induced pluripotent stem cells (hiPSCs) via customized designer [...] scientists from the LEBAO, together with scientists from the departments of Experimental Hematology and Human Genetics (both MHH), as well as with cooperation partners from the University Medical Center Freiburg [...] designer nuclease-based homologous recombination enables direct PCR screening for footprintless targeted human pluripotent stem cells. Stem Cell Reports 2, no. 1:107
Dynamic maturation traits of human g/d T cells Dynamic maturation traits of human g/d T cells The group of Sarina Ravens defined dynamic maturation traits of human g/d T cells, how this relates to the
development of diseases and to develop suitable therapies. In order to be able to transfer the results to humans, however, it must be ensured that the cell types and molecular signalling pathways studied in detail [...] described about 40 years ago as a cell type of its own in rat lungs. Whether such cells also exist in the human lung remained controversial in science. Now, a research team led by Professor Dr. Christian Mühlfeld [...] the physician - but only if the findings from lung research on rodents can actually be applied to humans. Final proof through a combination of different detection methods To answer that, the research team
Abdelilah-Seyfried, S., and Zweigerdt, R. 2024. Transient stabilization of human cardiovascular progenitor cells from human pluripotent stem cells in vitro reflects stage-specific heart development in [...] bioreactor. Application to human induced pluripotent stem cell culture. J Biomech . https://doi.org/10.1016/j.jbiomech.2019.07.021 . Manstein, F., Halloin, C., and Zweigerdt, R. 2019. Human Pluripotent Stem Cell [...] Gohring, G., Martin, U.* , and Merkert, S.*. 2018. Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation. Stem Cell Res 30:117. *authors contributed
cardiomyopathy using human induced pluripotent stem cells". Miriana Dardano from the RG Zweigerdt "Blood-generating heart-forming organoids (BG-HFOs) recapitulate the co-evolution of the human haematopoietic [...] "Development of an in vitro disease model for primary ciliary dyskinesia (PCD) using patient-derived human induced pluripotent stem cells". Rajesh Vivekanandan from the RG Gruh "Contribution of iPSC-derived
Paper in Nature Protocols “Cardiac differentiation of human pluripotent stem cells in scalable suspension culture”, Nature Protocols 2015 In August 2015, five LEBAO scientists conjointly released a publication [...] publication in the renowned journal Nature Protocols (impact factor 2015: 9,646). Cardiomyocytes based on human pluripotent stem cells are not only a promising potential cell source for regenerative therapies but [...] Kempf, H.*, Kropp, C.*, Olmer, R., Martin, U., and Zweigerdt, R. 2015. Cardiac differentiation of human pluripotent stem cells in scalable suspension culture. Nature Protocols 10, no. 9:1345. *Authors
Human heart-forming organoids recapitulate early heart and foregut development For the first time, MHH research group can use human stem cells to simulate early heart development in the cell culture dish [...] dish Scientists use so-called human pluripotent stem cells (hPSC) to study the early stages of embryonic development in the cell culture dish. These are cells with special properties that can be reproduced [...] the Hannover Medical School (MHH), succeeded in tracing the entire path to the early stages of a human heart in cell culture for the first time. The research work is published in the renowned journal Nature
application of human cardiac muscle cells, small animal models are unfortunately unsuitable, since the heart rate in these animals is much higher than in humans, and transplanted human muscle cells therefore [...] Mar 25;15(1):89. doi: 10.1186/s13287-024-03699-z Transient stabilization of human cardiovascular progenitor cells from human pluripotent stem cells in vitro reflects stage-specific heart development in [...] for individualized Cell Therapy with iPSC-derived Cardiomyocytes Preclinical evaluation in a non-human primate model and a roadmap for clinical application The Federal Ministry of Education and Research