the European Society of Gene and Cell Therapy (ESGCT) 4th Annual Congress of the German Society for Stem Cell Research (GSZ) 16th Annual meeting of the German Society for Gene Therapy (DG-GT) 2nd Meeting [...] combined meeting of the „European Society of Gene and Cell Therapy (ESGCT)“, the “German Society for Stem Cell Research (GSZ)“, the „German Society for Gene Therapy (DG-GT)“ and the „International Society [...] REBIRTH will take place on November 20-25, 2009 in Hannover. The main foci of the meeting include gene therapy, cell therapy, stem cell biology and regenerative medicine. Approx. 1,000 international p

Science Bio Informatics CRISPR/Cas9 gene editing in primary T cells Digital Fluorescence microscopy techniques, confocal microscopy and cell transfection Flow-cytometry Gene technology Gnotobiology and rederivation [...] capture of transcribed sequences [SCOTS] Proteome analysis, digital fluorescence microscopy Retroviral gene transfer Single cell immunophenotyping by ChipCytometry Software tools for imaging in infection S

the analysis of blood samples for the analysis of candidate genes of congenital neutropenias or for the analysis of the G-CSF receptor gene CSF3R in the context of our research projects. Please contact [...] leukemia development in blood cells of affected patients. The investigation of acquired mutations in the gene for the G-CSF receptor (CSF3R) in our laboratory has become an important standard diagnostic tool

would be the development of a gene therapy to treat this disease, which is called autosomal recessive complete IFN-γR1 deficiency. In Professor Lachmann's research group, gene shuttles were successfully developed [...] tested for their function by Liart Pollmann. He successfully introduced the missing gene into target cells by means of the gene shuttles and was able to demonstrate the restoration of the signaling cascade in [...] Disease (MSMD) Liart Pollmann, MD, PhD , has completed his doctoral thesis entitled "Human Lentiviral Gene Therapy Restores the Cellular Phenotype of Autosomal Recessive CompleteIFN-γR1 Deficiency" with Professor

mammalian cell culture; transfection; stable and transient gene expression; gene silencing (siRNA- or shRNA-mediated knock-down); CRISPR/Cas-mediated gene editing; cell toxicity assays Microscopy: epifluorescence

Leszek Lisowski „The future is here! The next generation of AAV vector as a foundation of cell and gene therapeutics of the future“ Associate Professor Lisowski received his PhD from Weill Cornell University [...] evaluation. In 2012, he was recruited by the Salk Institute for Biological Studies to oversee their Gene Transfer, Targeting and Therapeutic (GT3) facility. In 2015, he was recruited by the University of [...] purification, with special interest in clinical vector manufacturing. His team is leading a number of gene therapy programs that aim to develop novel advanced therapeutics for paediatric genetic disorders

affects about one in 6,500 newborns in Germany. It is caused by a mutation in the so-called SMN1 gene. If the gene is altered or completely lost, the body lacks the blueprint for the corresponding SMN protein [...] muscle atrophy, which in severe cases can lead to death in infancy if left untreated. Although the gene responsible for SMA is known, it is still unclear how exactly the loss of motor neurones occurs. An [...] switch," says Dr. Niko Hensel, first author of the study. To do this, the scientists silenced the SMN gene in the nematode Caenorhabditis elegans, which is only one millimetre in size. They then observed that

complexity arises from regulation and does not correlate with the number of genes. The lab focuses on the regulation of gene expression and RNA biology. In terms of viruses we dissect the regulation of [...] causing various human diseases. Both areas follow our central idea: A deeper knowledge of both viral gene expression and the pathomechanisms of non-coding mutations will advance our understanding of cellular [...] medal: Noncoding mutations reveal new pathological mechanisms and insights into the regulation of gene expression. Wiley Interdiscip Rev RNA. 2021 Jan;12(1):e1616. Review. 2. Krooss S, Werwitzke S, Kopp

customized designer nucleases has been shown to be significantly more efficient than conventional gene targeting, but still typically depends on the introduction of additional genetic selection. This genome [...] “Stem Cell Reports” in January 2014. In this study, the authors demonstrate a selection-independent gene targeting without the typically required antibiotic selection. This optimized approach allows targeted [...] and robust expression and enables the introduction of mutations on nucleotide level as well as the gene correction of disease-specific mutations in patient-derived iPSCs. This method is not only of utmost

the considerable polymorphism of the genes underlying the HLA complex, more than 24,000 alleles can currently be differentiated at the transplantation-relevant HLA gene sites A, B, C, DRB1, DQB1 and DPB1 [...] transplantation and in patients prior to stem cell transplantation if a mismatch is present in one of the genes relevant for stem cell transplantation (HLA-A, B, C, DRB1, DQB1, DPB1). In patients on the kidney [...] available. A donor is considered identical if at least one high-resolution identity exists for the genes HLA-A, B, C, DRB1, DQB1. More than nine million registered stem cell donors are currently available