Scattering (DLS). Microscopy: Fluorescence microscopy, DIC, TIRFM, Confocal Microscopy, Fluorescence Life Time Imaging (FLIM), Fluorescence Resonance Energy Transfer (FRET); Fluorescence Correlation Spectroscopy [...] and glial cells; D. discoideum ; immunohictochemistry; transfection Spectroscopy: Absorption and Fluorescence Spectroscopy Techniques; Time-resolved Spectroscopy; Polarization Spectroscopy; Circular Dichroism [...] protein expression; native and recombinant protein production (E. coli, D. discoideum, Sf9-cells); Fluorescence Labeling techniques; gene silencing; stable gene expression; polyclonal antibody production.

protein-protein interactions (Microscale thermophoresis, Fluorescence polarization/ anisotropy; Circular Dichroism, Calorimetry – ITC and DSC) Fluorescence microscopy based functional assays: TIRF; in vitro [...] diated gene editing; cell toxicity assays Microscopy: epifluorescence and TIRF microscopy of fluorescently labelled proteins, protein complexes and cells; Confocal Microscopy; FRAP; FRET; Live Cell Imaging

interference optical system and a Beckman Coulter ProteomeLab XL-I equipped with an Aviv Biomedical fluorescence detection system. Analytical ultracentrifugation can provide the following information about [...] interactions determination of affinity constants of homo- and hetero-interactions investigation of fluorescently labeled proteins in complex mixtures like serum accessible concentration range from millimolar

Laboratory Animal Science Bio Informatics CRISPR/Cas9 gene editing in primary T cells Digital Fluorescence microscopy techniques, confocal microscopy and cell transfection Flow-cytometry Gene technology [...] bacterial RNA for the selective capture of transcribed sequences [SCOTS] Proteome analysis, digital fluorescence microscopy Retroviral gene transfer Single cell immunophenotyping by ChipCytometry Software tools

equilibrium AUC) Differential scanning fluorescence (Thermo-Fluor assay) Single molecule and live-cell imaging approaches Confocal, evanescent field and spinning disk fluorescence microscopy Macromolecular X-ray

gation In vitro DNA replication Protein expression and purification Side directed mutagenesis Fluorescence spectrophotometry UV/Vis spectrophotometry Isothermal titration calorimetry

Methods Super-Resolution Fluorescence Microscopy / Nanoscopy ( d STORM, DNA-PAINT, QuantumDot Triexciton Imaging, expansion Microscopy) Electrophoretic Nanoinjection FRET / smFRET

ImageXpress A widefield fluorescence microscope integrating hardware and software for automated acquisition and analysis of images for high-throughput cellbased screening in 96 and 384 well plates. Other

assay The fluorescently marked actin filaments translocated by surface immobilised myosin II motors. © 2019 [Mamta Amrute] [Translate to Englisch:] Single molecule processivity assay Fluorescently labelled [...] project that involved studying molecular motors using single-molecule fluorescence detection technique, Total internal reflection fluorescence microscopy. For my post-doctoral research, I moved to Medical Research [...] experimental approaches include single molecule biophysical methods such as total internal reflection fluorescence microscopy (TIRFM), zero mode waveguides and optical trapping to obtain precise kinetic and mechanical

the lab works on the cell biology of Herpes viruses assembly with a strong focus on live cell fluorescence microscopy and electron microscopy. The scheme illustrates the assembly pathways of Herpes viruses