markers, such as beta-MyHC. The matured cardiomyocytes are then screened for potential differences in gene expression, contractile features, calcium transients and alignment of myofibrils between hiPSCs-CMs
Pokrant T., Faix J., Di Donato N., Taft M.H., Manstein D.J. (2022) Frameshift mutation S368fs in the gene encoding cytoskeletal β-actin leads to ACTB -associated syndromic thrombocytopenia by impairing actin [...] Taft, M. H., Manstein, D.J. and Henn, A. (2017) N-terminal splicing extensions of the human MYO1C gene fine-tune the kinetics of the three full-length myosin IC isoforms. J Biol Chem, 292, 17804-17818 [...] P. & Hunt, D.M. (1995) Cloning vectors for the production of proteins in Dictyostelium discoideum Gene 162 , 129-134. ( Abstract ) ( pdf, 436 kB) Schröder, R.R., Manstein, D.J., Jahn, W., Holden, H.M.
(GlaxoSmithKline, UK). The aim of my PhD research was to improve targeted retroviral vectors used in gene therapy. Then I started studying herpesviruses, working first with Kaposi’s sarcoma-associated herpesvirus
protocols are used to generate proximal and distal respiratory epithelium from hiPSCs. CRISPR/Cas9 based gene editing will be applied to knock out NRF2, which induces a major cytoprotective program and is the
Vanaudenaerde B, Xu Y, Hohlfeld JM, Krug N, Herazo-Maya JD, Rottoli P, Wuyts WA, Kaminski N. BAL cell gene expression is indicative of outcome and airway basal cell involvement in idiopathic pulmonary fibrosis
M, Ravens I, Fuss J, Hansen G, Addo MM, Förster R. Blood transcriptome profiling reveals distinct gene networks induced by mRNA vaccination against COVID-19. Eur J Immunol. 2024 Nov;54(11):e2451236. doi: [...] Förster R, Bauersachs J, Ola R, Dobreva G, Völkers M, Heineke J. Analysis of myocardial cellular gene expression during pressure overload reveals matrix based functional intercellular communication. iScience [...] 35281736; PMCID: PMC8908217. Rezalotfi A, Fritz L, Förster R, Bošnjak B. Challenges of CRISPR-Based Gene Editing in Primary T Cells. Int J Mol Sci. 2022 Feb 1;23(3):1689. doi: 10.3390/ijms23031689. PMID:
Gottlieb J, Welte T, Braubach P, Laenger F, Hoeper MM, Kuehnel MP, Jonigk DD. Comparative analysis of gene expression in fibroblastic foci in patients with idiopathic pulmonary fibrosis and pulmonary sarcoidosis
exon 12, a heterozygous in-frame deletion in exon 3 and a missense mutation in exon 11 of the BMPR2 gene. Stem Cell Res. 2021;55:102488. https://pubmed.ncbi.nlm.nih.gov/34388490/ . Kokeny G, Calvier L, Hansmann
Institute for Experimental Hematology as a W3 professor and world-renowned researcher in cell and gene therapy in the blood-forming system. As a research dean, he headed the research commission of the
Central PMCID: PMC5460037 2016: Georgiev H, Ravens I, Benarafa C, Förster R, Bernhardt G. Distinct gene expression patterns correlate with developmental and functional traits of iNKT subsets. Nat Commun