composition of sarcomeric proteins. Subcellular myofibrils can be obtained from human (e.g., biopsies), human-derived and non-human cardiac and skeletal small muscle samples (e.g., mouse, rat, rabbit, zebrafish [...] Contraction-relaxation function and mechano-chemical coupling of myofibrils Investigations of human, human pluripotent stem cell-derived cardiomyocytes and animal contractile models in non-pathologic and [...] zebrafish and hESC-/hiPSC-CMs). In vitro -differentiated cardiomyocytes . Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold great potential for the treatment of cardiovascular diseases by cell

on enables direct PCR screening for footprintless targeted human pluripotent stem cells”, Stem Cell Reports 2014 Genetic engineering of human induced pluripotent stem cells (hiPSCs) via customized designer [...] scientists from the LEBAO, together with scientists from the departments of Experimental Hematology and Human Genetics (both MHH), as well as with cooperation partners from the University Medical Center Freiburg [...] designer nuclease-based homologous recombination enables direct PCR screening for footprintless targeted human pluripotent stem cells. Stem Cell Reports 2, no. 1:107

Paper in Nature Protocols “Cardiac differentiation of human pluripotent stem cells in scalable suspension culture”, Nature Protocols 2015 In August 2015, five LEBAO scientists conjointly released a publication [...] publication in the renowned journal Nature Protocols (impact factor 2015: 9,646). Cardiomyocytes based on human pluripotent stem cells are not only a promising potential cell source for regenerative therapies but [...] Kempf, H.*, Kropp, C.*, Olmer, R., Martin, U., and Zweigerdt, R. 2015. Cardiac differentiation of human pluripotent stem cells in scalable suspension culture. Nature Protocols 10, no. 9:1345. *Authors

cardiomyopathy using human induced pluripotent stem cells". Miriana Dardano from the RG Zweigerdt "Blood-generating heart-forming organoids (BG-HFOs) recapitulate the co-evolution of the human haematopoietic [...] "Development of an in vitro disease model for primary ciliary dyskinesia (PCD) using patient-derived human induced pluripotent stem cells". Rajesh Vivekanandan from the RG Gruh "Contribution of iPSC-derived

thesis: „Effects of Culture Conditions and Cardiomyogenic Differentiation on the (Glyco-) Proteome of Human Pluripotent Stem Cells“ 2010: diploma thesis at the Diplomarbeit Institute for Clinical Biochemistry [...] imbalance among cardiomyocytes as pathogenic factor for Hypertrophic Cardiomyopathy – investigations on human pluripotent stem-cell derived cardiomyocytes carrying MyBP-C-mutations. The current project focuses [...] future therapeutic approaches. Expertise Scientific expertise: Cultivation and differentiation of human induced pluripotent stem cells (hiPSCs), particularly to cardiomyocytes and skeletal muscle cells

advanced protocol for the generation of heart-forming organoids from human pluripotent stem cells Heart-forming organoids (HFOs) derived from human pluripotent stem cells are a complex, highly structured in vitro [...] vitro model of early heart, foregut and vasculature development. In the human embryo, these tissues emerge in close proximity to each other and rely on signal exchange for proper development. HFOs represent

specialist in laboratory medicine, together with Marie she is also working on better characterization of human immune cells. Nils Funk Funk.Nils @ mh-hannover.de MD Student Nils is an MD student who is working [...] senescence. Andreas Penndorf andreas.penndorf @ stud.uni-hannover.de MSc Student Andreas studies human natural killer cell immunity in close collaboration with the central laboratory of Hannover Medical [...] stud.mh-hannover.de MD Student Marie is an MD student who is working on better characterization of human immune cells.

Human heart-forming organoids recapitulate early heart and foregut development For the first time, MHH research group can use human stem cells to simulate early heart development in the cell culture dish [...] dish Scientists use so-called human pluripotent stem cells (hPSC) to study the early stages of embryonic development in the cell culture dish. These are cells with special properties that can be reproduced [...] the Hannover Medical School (MHH), succeeded in tracing the entire path to the early stages of a human heart in cell culture for the first time. The research work is published in the renowned journal Nature

onal human pluripotent stem cell-derived cardiomyocyte models for investigating cardiac hypertrophy” Wiebke Triebert (RG Zweigerdt): “Bioprocess development for GMP-compliant production of human pluripotent [...] cell-derived cardiomyocytes” Kevin Ullmann (RG Zweigerdt): “Physical and Physiological Control of Human Pluripotent Stem Cells Culture” Mark-Christian Jaboreck (RG Martin): “Development of a hiPSC-based

Infektiologie und Endokrinologie ‘Generation of multicellular human liver microtissue models using organoid-derived and direct reprogrammed human hepatocyte-like cells’ Fawaz Ahmad Saleh from the Department [...] Biotechnology & Artificial Organs (LEBAO) ‘Establishing a scalable bioreactor platform to generate human iPSC-derived hematopoietic cell types for regenerative applications’ Ana-Maria Zamfirescu from the