thesis: „Effects of Culture Conditions and Cardiomyogenic Differentiation on the (Glyco-) Proteome of Human Pluripotent Stem Cells“ 2010: diploma thesis at the Diplomarbeit Institute for Clinical Biochemistry [...] imbalance among cardiomyocytes as pathogenic factor for Hypertrophic Cardiomyopathy – investigations on human pluripotent stem-cell derived cardiomyocytes carrying MyBP-C-mutations. The current project focuses [...] future therapeutic approaches. Expertise Scientific expertise: Cultivation and differentiation of human induced pluripotent stem cells (hiPSCs), particularly to cardiomyocytes and skeletal muscle cells
on enables direct PCR screening for footprintless targeted human pluripotent stem cells”, Stem Cell Reports 2014 Genetic engineering of human induced pluripotent stem cells (hiPSCs) via customized designer [...] scientists from the LEBAO, together with scientists from the departments of Experimental Hematology and Human Genetics (both MHH), as well as with cooperation partners from the University Medical Center Freiburg [...] designer nuclease-based homologous recombination enables direct PCR screening for footprintless targeted human pluripotent stem cells. Stem Cell Reports 2, no. 1:107
Dynamic maturation traits of human g/d T cells Dynamic maturation traits of human g/d T cells The group of Sarina Ravens defined dynamic maturation traits of human g/d T cells, how this relates to the
Abdelilah-Seyfried, S., and Zweigerdt, R. 2024. Transient stabilization of human cardiovascular progenitor cells from human pluripotent stem cells in vitro reflects stage-specific heart development in [...] bioreactor. Application to human induced pluripotent stem cell culture. J Biomech . https://doi.org/10.1016/j.jbiomech.2019.07.021 . Manstein, F., Halloin, C., and Zweigerdt, R. 2019. Human Pluripotent Stem Cell [...] Gohring, G., Martin, U.* , and Merkert, S.*. 2018. Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation. Stem Cell Res 30:117. *authors contributed
cardiomyopathy using human induced pluripotent stem cells". Miriana Dardano from the RG Zweigerdt "Blood-generating heart-forming organoids (BG-HFOs) recapitulate the co-evolution of the human haematopoietic [...] "Development of an in vitro disease model for primary ciliary dyskinesia (PCD) using patient-derived human induced pluripotent stem cells". Rajesh Vivekanandan from the RG Gruh "Contribution of iPSC-derived
Paper in Nature Protocols “Cardiac differentiation of human pluripotent stem cells in scalable suspension culture”, Nature Protocols 2015 In August 2015, five LEBAO scientists conjointly released a publication [...] publication in the renowned journal Nature Protocols (impact factor 2015: 9,646). Cardiomyocytes based on human pluripotent stem cells are not only a promising potential cell source for regenerative therapies but [...] Kempf, H.*, Kropp, C.*, Olmer, R., Martin, U., and Zweigerdt, R. 2015. Cardiac differentiation of human pluripotent stem cells in scalable suspension culture. Nature Protocols 10, no. 9:1345. *Authors
Human heart-forming organoids recapitulate early heart and foregut development For the first time, MHH research group can use human stem cells to simulate early heart development in the cell culture dish [...] dish Scientists use so-called human pluripotent stem cells (hPSC) to study the early stages of embryonic development in the cell culture dish. These are cells with special properties that can be reproduced [...] the Hannover Medical School (MHH), succeeded in tracing the entire path to the early stages of a human heart in cell culture for the first time. The research work is published in the renowned journal Nature
application of human cardiac muscle cells, small animal models are unfortunately unsuitable, since the heart rate in these animals is much higher than in humans, and transplanted human muscle cells therefore [...] Mar 25;15(1):89. doi: 10.1186/s13287-024-03699-z Transient stabilization of human cardiovascular progenitor cells from human pluripotent stem cells in vitro reflects stage-specific heart development in [...] for individualized Cell Therapy with iPSC-derived Cardiomyocytes Preclinical evaluation in a non-human primate model and a roadmap for clinical application The Federal Ministry of Education and Research
advanced protocol for the generation of heart-forming organoids from human pluripotent stem cells Heart-forming organoids (HFOs) derived from human pluripotent stem cells are a complex, highly structured in vitro [...] vitro model of early heart, foregut and vasculature development. In the human embryo, these tissues emerge in close proximity to each other and rely on signal exchange for proper development. HFOs represent
specialist in laboratory medicine, together with Marie she is also working on better characterization of human immune cells. Nils Funk Funk.Nils @ mh-hannover.de MD Student Nils is an MD student who is working [...] senescence. Andreas Penndorf andreas.penndorf @ stud.uni-hannover.de MSc Student Andreas studies human natural killer cell immunity in close collaboration with the central laboratory of Hannover Medical [...] stud.mh-hannover.de MD Student Marie is an MD student who is working on better characterization of human immune cells.