Fedorov R, Dirr L, Führing JI (2024). Identification and characterization of the functional tetrameric UDP-glucose pyrophosphorylase from Klebsiella pneumoniae . mBio. 2024 Dec 20:e0207124. doi: 10.1128/mbio [...] Tetramerization is essential for the enzymatic function of the Pseudomonas aeruginosa virulence factor UDP-glucose pyrophosphorylase. mBio. 2024 Mar 12:e0211423. doi: 10.1128/mbio.02114-23. Online ahead of [...] FH, Guerin ME, Fuehring J , Etxebeste O, Connell SR (2023). CryoEM analysis of the essential native UDP-glucose pyrophosphorylase from Aspergillus nidulans reveals key conformations for activity regulation

(2008). A single Caenorhabditis elegans Golgi apparatus-type transporter of UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine, and UDP-N-acetylgalactosamine. Biochemistry, 47, 4337-4344. Schmalhorst,P.S., [...] H. and Ashikov, A. (2014). UDP-Xylose and UDP-N-Acetylglucosamine Transporter (SLC35B4). Handbook of Glycosyltransferases and Related Genes 1393-1402. Bakker, H. (2014). UDP-Glucuronate Decarboxylase 1 [...] Strasser, R., Bakker, H., Schmulling, T., and Werner, T. (2015). Arabidopsis ROCK1 transports UDP-GlcNAc/UDP-GalNAc and regulates ER protein quality control and cytokinin activity. Proc. Natl Acad. Sci

Current Projects Allosteric regulation of UDP-sugar pyrophosphorylases as a means to develop new anti-parasitic treatments UDP-sugar pyrophosphorylases play a central role in cellular carbohydrate biosynthesis [...] biosynthesis. As metabolic switches, UDP-sugar pyrophosphorylases are subject to elaborate regulatory mechanisms. Elucidating these mechanisms is essential for the fundamental understanding of cellular c [...] enzymes. Due to their central position in carbohydrate biosynthesis and species-specific differences, UDP-sugar pyrophosphorylases are attractive targets for the development of new drugs against protozoan

monosaccharides into nucleotide sugars. The enzyme UDP-glucose pyrophosphorylase (UGP) catalyzes the synthesis of UDP-glucose, which in turn gives rise to further UDP-activated sugars, all of which are involved [...] Research Our group aims at a detailed characterization of the enzyme UDP-glucose pyrophosphorylase (UGP) , a candidate drug target, from pathogenic species and the human host to identify structural and/or

heraus, dass beim Zelltod der Hepatozyten bestimmte gespeicherte, aktivierte Zuckermoleküle (UDP-Glucose und UDP-Galactose) freigesetzt werden. Diese können als sogenannte Liganden passgenau an ein Protein

Rita Gerardy-Schahn, Cellular Chemistry, OE 4330, Project: Development of inhibitors of the UDP-Glucose/UDP-Galactose biosynthesis in Trypanosomatids. Final exam June 2019. Dhanya Ramachandran INDIA R

Oelmann S., Gerardy-Schahn R.*, and Bakker H ., (2005), ER retention of the large splice variant of the UDP-galactose transporter is caused by a di-lysine motif, Glycobiology 2005, Abteilung Zelluläre Chemie

J, Shepherd SM, Eadsforth TC, Gray D, Fedorov R and Routier FH. Identification of Leishmania major UDP-Sugar Pyrophosphorylase Inhibitors Using Biosensor-Based Small Molecule Fragment Library Screening [...] and Fedorov R. A Quaternary Mechanism Enables the Complex Biological Functions of Octameric Human UDP-glucose Pyrophosphorylase, a Key Enzyme in Cell Metabolism. (2015) Sci. Rep . 5 , 9618; DOI:10.103 [...] Lamerz A-C, Baruch P, Gerardy-Schahn R and Fedorov R. Catalytic mechanism and allosteric regulation of UDP-glucose pyrophosphorylase from Leishmania major . (2013) ACS Catalysis . 3 :2976-2985. Hellert J,

(2008) A Single Caenorhabditis elegans Golgi Apparatus-Type Transporter of UDP-Glucose, UDP-Galactose, UDP-N-Acetylglucosamine, and UDP-N-Acetylgalactosamine. Biochemistry 47 (14):4337-4344. Drake, P.M. , Nathan [...] F., Turk, J., Beverley, S.M., and Routier, F.H. (2010) Deletion of UDP-Glucose Pyrophosphorylase Reveals a UDP-Glucose Independent UDP-Galactose Salvage Pathway in Leishmania major . Glycobiology . 20 [...] A, Liu H, Griffith CL, Bakker H , Doering TL (2016) Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter Glycobiology 10.1093/glycob/cww078 Martin NT , Martin MU (2016). Interleukin

Florence Fassy, F., Schönbrunn, E. (2005). A novel inhibitor that suspends the induced-fit mechanism of UDP-N-acetylglucosamin enolpyruvyl transferase (MurA). J. Biol. Chem. 280 , 14070-14075. Eschenburg, S [...] S. , Priestman, M.A., Schönbrunn, E. (2005). Evidence that the fosfomycin target Cys115 in UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is essential for product release. J. Biol. Chem. 280 , 3757-3763 [...] Eschenburg, S. , Perrakis, A., Krekel, F., Amrhein, N., Mandelkow, E. (1996). Crystal structure of UDP-N-acetylglucosamine enolpyruvyl transferase, the target of the antibiotic fosfomycin. Structure 4