Total synthesis of FAD and FMN analogues and their application for probing stereo-specificities of flavin cofactors in enzymatic reactions.
Pioneering work on the molecular genetic manipulation of the model organism Dictyostelium discoideum.
Key contributions to the development and application of molecular genetics, transient kinetics, and hybrid structural biology approaches for the elucidation of myosin and dynamin function, structure, and regulation.
Establishment of the first recombinant expression system for the production of functional myosin motors.
Application of protein engineering approaches leading to the elucidation of the fundamental principles governing myosin directionality and chemomechanical coupling.
Engineering of single-polypeptide myosin motors with enhanced stability and motility for use in biohybrid devices.
Structural characterization of the actomyosin complexes produced in Dictyostelium and human cells and elucidation of their involvement in cytokinesis and cell motility.
Structure-based identification of multi-site allostery in the myosin motor domain and characterization of several class-specific small molecule modulators of cytoskeletal myosins.
First description of a pharmacological chaperone for cardiac ß-myosin. Addition of the small molecule to the functionally impaired target protein leads to full restoration of myosin motor activity in a time and concentration dependent manner.
Elucidation of the function of cytoskeletal tropomyosins in controlling the motor activity and dynamic behavior of myosins in an isoform-specific manner.