Microbial glycobiochemistry and vaccine development


Alcalá-Orozco EA, Grote V, Fiebig T, Klamt S, Reichl U, Rexer TFT (2023). A cell-free multi-enzyme cascade for the synthesis of CDP-glycerol. Chembiochem. 2023 Aug 14:e202300463. doi: 10.1002/cbic.202300463. Online ahead of print. PMID: 37578628

Cifuente JO, Schulze J, Bethe A, Di Domenico V, Litschko C, Budde I, Eidenberger L, Thiesler H, Ramón Roth I, Berger M, Claus H, D'Angelo C, Marina A, Gerardy-Schahn R, Schubert M, Guerin ME, Fiebig T (2023). A multi-enzyme machine polymerizes the Haemophilus influenzae type b capsule. Nat Chem Biol. 2023 Jun 5. doi: 10.1038/s41589-023-01324-3. Online ahead of print. PMID: 37277468

Sulewska M, Berger M, Damerow M, Schwarzer D, Buettner FFR, Bethe A, Taft MH, Bakker H, Mühlenhoff M, Gerardy-Schahn R, Priem B, Fiebig T (2023). Extending the enzymatic toolbox for heparosan polymerization, depolymerization, and detection. Carbohydr Polym. 2023 Nov 1;319:121182. doi: 10.1016/j.carbpol.2023.121182. Epub 2023 Jul 8. PMID: 37567694


Bense S, Witte J, Preuße M, Koska M, Pezoldt L, Dröge A, Hartmann O, Müsken M, Schulze J, Fiebig T, Bähre H, Felgner S, Pich A, Häussler S (2022). Pseudomonas aeruginosa post-translational responses to elevated c-di-GMP levels. Mol Microbiol. 2022 May;117(5):1213-1226. doi: 10.1111/mmi.14902. Epub 2022 Apr 11. PMID: 35362616


Litschko C, Budde I, Berger M, Bethe A, Schulze J, Alcala Orozco EA, Mahour R, Goettig P, Führing JI, Rexer T, Gerardy-Schahn R, Schubert M, Fiebig T (2021). Mix-and-Match System for the Enzymatic Synthesis of Enantiopure Glycerol-3-Phosphate-Containing Capsule Polymer Backbones from Actinobacillus pleuropneumoniae, Neisseria meningitidis, and Bibersteinia trehalosi. mBio. 2021 May 26:e0089721. doi: 10.1128/mBio.00897-21. Online ahead of print. PMID: 34076489


Bartling, B., Brüchle, N.C., Rehfeld, J.S., Boßmann, D., Fiebig, T., Litschko, C., Fohrer, J., Gerardy-Schahn, R., Scheper, T., and Beutel, S. (2020). Accelerated production of α2,8- and α2,9-linked polysialic acid in recombinant Escherichia coli using high cell density cultivation. Biotechnol. Reports 28, e00562. DOI: 10.1016/j.btre.2020.e00562

Budde I, Litschko C, Führing JI, Gerardy-Schahn R, Schubert M, and Fiebig T. (2020), An enzyme-based protocol for cell-free synthesis of nature-identical capsular oligosaccharides from Actinobacillus pleuropneumoniae serotype 1. J Biol Chem 2020 Apr 24;295(17):5771–84. DOI: 10.1074/jbc.RA120.012961

Fiebig, T., Cramer, J.T., Bethe, A., Baruch, P., Curth, U., Führing, J.I., Buettner F.F.R., Vogel, U., Schubert, M., Fedorov, R., Mühlenhoff, M., (2020), Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A, Nat Commun. 2020 Sep 18;11(1):4723. doi: 10.1038/s41467-020-18464-y

Litschko, C., Budde, I., Berger, M., and Fiebig, T. (2021). Exploitation of Capsule Polymerases for Enzymatic Synthesis of Polysaccharide Antigens Used in Glycoconjugate Vaccines. Methods Mol. Biol. 2183, 313–330. DOI: 10.1007/978-1-0716-0795-4_16


Litschko C, Oldrini D, Budde I, Meens J, Gerardy-Schahn R, Berti F, Schubert M, and Fiebig T. (2018), A New Family of Capsule Polymerases Generates Teichoic Acid-Like Capsule Polymers in Gram-Negative Pathogens. MBio 2018 May 29;9(3):e00641-18. DOI: 10.1128/mBio.00641-18

Oldrini D, Fiebig T, Romano MR, Proietti D, Berger M, Tontini M, De Ricco R, Santini L, Morelli L, Lay L, Gerardy-Schahn R, Berti F, and Adamo R. (2018), Combined Chemical Synthesis and Tailored Enzymatic Elongation Provide Fully Synthetic and Conjugation-Ready Neisseria meningitidis Serogroup X Vaccine Antigens. ACS Chem Biol 2018 Apr 20;13(4):984–94. DOI: 10.1021/acschembio.7b01057

Fiebig T, Litschko C, Freiberger F, Bethe A, Berger M, Gerardy-Schahn R., (2018), Efficient solid-phase synthesis of meningococcal capsular oligosaccharides enables simple and fast chemoenz.


Fiebig T, Romano MR, Oldrini D, Adamo R, Tontini M, Brogioni B, Santini L, Berger M, Costantino P, Berti F, and Gerardy-Schahn R., (2016), An efficient cell free enzyme-based total synthesis of a meningococcal vaccine candidate. NPJ vaccines 2016 Dec 15;1(1):16017. DOI: 10.1038/npjvaccines.2016.17


Litschko C, Romano MR, Pinto V, Claus H, Vogel U, Berti F, Gerardy-Schahn R, and Fiebig T., (2015), The capsule polymerase CslB of Neisseria meningitidis serogroup L catalyzes the synthesis of a complex trimeric repeating unit comprising glycosidic and phosphodiester linkages. J Biol Chem 2015 Oct 2;290(40):24355–66. DOI: 10.1074/jbc.M115.678094


Fiebig T, Freiberger F, Pinto V, Romano MR, Black A, Litschko C, Bethe A, Yashunsky D, Adamo R, Nikolaev A, Berti F, and Gerardy-Schahn R., (2014), Molecular cloning and functional characterization of components of the capsule biosynthesis complex of Neisseria meningitidis serogroup A: toward in vitro vaccine production. J Biol Chem 2014 Jul 11;289(28):19395–407. DOI: 10.1074/jbc.M114.575142

Fiebig T, Berti F, Freiberger F, Pinto V, Claus H, Romano MR, Proietti D, Brogioni B, Stummeyer K, Berger M, Vogel U, Costantino P, and Gerardy-Schahn R., (2014), Functional expression of the capsule polymerase of Neisseria meningitidis serogroup X: a new perspective for vaccine development. Glycobiology 2014 Feb;24(2):150–8. DOI: 10.1093/glycob/cwt102


Haselhorst T, Fiebig T, Dyason JC, Fleming FE, Blanchard H, Coulson BS, and von Itzstein M., (2011), Recognition of the GM3 ganglioside glycan by Rhesus rotavirus particles. Angew Chem Int Ed Engl 2011 Feb 1;50(5):1055–8.  DOI: 10.1002/anie.201004116