Arnab Nayak

Dr. rer. nat.

Kontakt

Ph.D. Arnab Nayak

Tel:  +49 511 532 -2094 (Büro), -3654 (Labor)

Fax: +49 511 532 -4296

Adresse: 30625 Hannover, Carl-Neuberg-Str.1

Büro:  Gebäude J03, Block 01, Ebene 03, Raum 1340


Werdegang 

  • 2017: Joined the Institute of Molecular and Cell Physiology, Medical School Hannover. Currently, Principle Investigator of the Chromatin and SUMO physiology group
  • 2011 - 2017:  Postdoctoral Postdoctoral researcher in Goethe University Frankfurt.
  • 2009 – 2011: Research associate, University of Cambridge.
  • 2008-2009: Career Development Fellow (CDF), MRC-LMB, Cambridge.
  • 2004-2008: PhD in the University of Würzburg.
  • 2001-2003: Junior Research Fellow in National Centre for Cell Science (NCCS), India.
  • 1999-2001: Master in Zoology (specialization in Molecular, Applied and Clinical Genetics) in BHU, India.
  • 1996-1999: Bachelor in Zoology (with Chemistry and Botany), University of Burdwan, India.

 

Aktuelle Forschungsschwerpunkte

Cancer biology, Chromatin & Epigenetics, Molecular motor protein, Proteomics, Stem cells, SUMOylation, Single molecule studies of molecular motor proteins.

 

Expertisen

  • Cell biology, Biochemistry and Molecular Biology
    Human cord blood stem cell and mouse lymphocytes (CD4+ T cells, CD4+CD25+ regulatory T cells) isolation, culture. FACS. Generation of stable cell line (conditional protein expression, Flip-in tet-on and tet-off system). Gene-specific proteomics by exploiting CRISPR/Cas9 genome editing tool, 2-D gel electrophoresis, chromatin immunoprecipitation (ChIP), ChIP-Seq, coimmunoprecipitation, reporter gene activity assay, western Hybridization. Molecular cloning, DNA sequencing, and site-directed mutagenesis. Tandem affinity purification (TAP) of bait proteins followed by mass spectrometric analysis. Real time qPCR analysis of homeobox gene expression. Expression (in E. coli. and mammalian cells) and purification of recombinant protein by affinity chromatography, siRNA-mediated protein knock down.
     
  • Orbitrap elite mass spectrometers and related softwares, SILAC-based studies, label-free quantifications, Di-gly SUMO proteomics
  • Microscopy
    Confocal laser scanning microscopy. Total Internal Reflection Fluorescence (TIRF) microscopy.

 

Wissenschaftliche Artikel in Fachzeitschriften

  • Wang T, Brenner B, Nayak A, and Amrute-Nayak M, Acto-Myosin Cross-Bridge Stiffness Depends on the Nucleotide State of Myosin II, Nano Letters, 2020 Sep 15. doi: 10.1021/acs.nanolett.0c02960. [Online ahead of print] PubMed
  • Nayak A, Wang T, Franz P, Steffen W, Chizhov I, Tsiavaliaris G, Amrute-Nayak M, Single-molecule analysis reveals that regulatory light chains fine-tune skeletal myosin-II function, J Biol Chem. 2020 Apr 9. pii: jbc.RA120.012774. doi: 10.1074/jbc.RA120.012774, PMID: 32273340, Artikel, PubMed
  • Nayak A*, Lopez-Davila AJ, Kefalakes E, Holler T, Kraft T, Amrute-Nayak M. (2019). Regulation of SETD7 Methyltransferase by SENP3 is Crucial for Sarcomere Organization and Cachexia. Cell Reports. Vol 27, issue 9, P2725-2736.e4, May 28. (*corresponding author)
  • Nayak, A*., Reck, A., Morsczeck, C., and Muller, S. (2017). Flightless-I governs cell fate by recruiting the SUMO isopeptidase SENP3 to distinct HOX genes. Epigenetics Chromatin .10, 15. (*corresponding author)
  • Nayak, A., Viale-Bouroncle, S., Morsczeck, C., and Muller, S. (2014). The SUMO-specific isopeptidase SENP3 regulates MLL1/MLL2 methyltransferase complexes and controls osteogenic differentiation. Mol Cell 55, 47-58
  • Nayak, A., Glockner-Pagel, J., Vaeth, M., Schumann, J.E., Buttmann, M., Bopp, T., Schmitt, E., Serfling, E., and Berberich-Siebelt, F. (2009). Sumoylation of the transcription factor NFATc1 leads to its subnuclear relocalization and interleukin-2 repression by histone deacetylase. J Biol Chem 284, 10935-10946.
  • Muller, S., and Nayak, A*. (2016). Inhibition of MLL1 histone methyltransferase brings the developmental clock back to naive pluripotency. Stem Cell Investig 3, 58. (*corresponding author).
  • Nayak, A., and Muller, S. (2014). SUMO-specific proteases/isopeptidases: SENPs and beyond. Genome Biol 15, 422.
  • Raman, N., Nayak, A*., and Muller, S. (2013). The SUMO system: a master organizer of nuclear protein assemblies. Chromosoma 122, 475-485 (* - shared first author).
  • Amrute-Nayak M, Nayak A, Steffen W, Tsiavaliaris G, Scholz T, Brenner B. (2019). Transformation of Conventional, Non-processive Myosin II into a Fast Processive Motor. (2019). Small. Feb;15(7)
  • Jung, J., Nayak, A., Schaeffer, V., Starzetz, T., Kirsch, A.K., Muller, S., Dikic, I., Mittelbronn, M., and Behrends, C. (2017). Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. Elife 6
  • Raman, N., Nayak, A., and Muller, S. (2014). mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3. Mol Cell Biol 34, 4474-4484
  • Schmidt, D., Nayak, A., Schumann, J.E., Schimpl, A., Berberich, I., and Berberich-Siebelt, F. (2008). Blimp-1Deltaexon7: a naturally occurring Blimp-1 deletion mutant with auto-regulatory potential. Exp Cell Res 314, 3614-3627.
  • Tamura, N., Simon, J.E., Nayak, A., Shenoy, R., Hiroi, N., Boilot, V., Funahashi, A., and Draviam, V.M. (2015). A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset. Biol Open 4, 155-169
  •  Azukizawa, H., Dohler, A., Kanazawa, N., Nayak, A., Lipp, M., Malissen, B., Autenrieth, I., Katayama, I., Riemann, M., Weih, F., et al. (2011). Steady state migratory RelB+ langerin+ dermal dendritic cells mediate peripheral induction of antigen-specific CD4+ CD25+ Foxp3+ regulatory T cells. Eur J Immunol 41, 1420-1434.
  • Brandes, R.P., Harenkamp, S., Schurmann, C., Josipovic, I., Rashid, B., Rezende, F., Lowe, O., Moll, F., Epah, J., Eresch, J., Nayak A, et al. (2016). The Cytosolic NADPH Oxidase Subunit NoxO1 Promotes an Endothelial Stalk Cell Phenotype. Arterioscler Thromb Vasc Biol 36, 1558-1565.

 

Preise

  • Individual Research grant from the German Research Foundation (DFG).
  • Best talk award at UCT science symposium (Goethe University Frankfurt).
  • Fritz Thyseen foundation research grant for my postdoctoral study.
  • Career Development Fellowship (CDF) award from MRC-LMB, Cambridge, UK.
  • Best poster award at EMBO conference on Chromatin and Epigenetics 2007, EMBL, Heidelberg, Germany.
  • Fellowships from Graduate School of Immunomodulation (Würzburg University) and Hannover Biomedical Research School (HBRS, MHH) Germany for doctoral studies.
  • Junior Research Fellowship award by CSIR (Centre for Scientific and Industrial Research)- NET (National Eligibility Test), India.
  • GATE (Graduate Aptitude Test for Engineering) fellowship.
  • Junior research fellowship by NCCS Pune, India.
  • National Talent Search scholarship, India.

 

Eingeladene Vortragspräsentation auf internationalen Konferenzen

  • EMBO conference on Chromatin and Epigenetics (EMBL Heidelberg 2007).
  • Cambridge Cancer Centre meeting, Cambridge (UK, 2010).
  • DFG conference at Zeuthen (2013).
  • UCT science day symposium (Goethe University Frankfurt, 2014).
  • International Congress on Epigenetics & Chromatin (Frankfurt, 2017).
  • Invited speaker at Indian Institute of Scientific Education & Research (IISER-Kolkata, 2018).
  • Gordon Research Conference (short talk) on Myogenesis, Lucca Italy (2019).

 

Förderungen

German Research Foundation (DFG)- NA 1565/2-1. seit 2019

 

Lehre

  • From 2019, annual practical/teaching course in Mechanik/Wärme (Muscle physiology) for medical students and “Myogenesis” for Biomedicine students at Hannover Medical School.
  • From 2012-2016, annual practical course-work in Biochemistry for medical students in Universitäts Klinikum, Goethe-Universität Frankfurt.
  • 2006 and 2007, practical/teaching course in T-cell activation and confocal microscopy in Würzburg University (Germany).