mammalian cell culture; transfection; stable and transient gene expression; gene silencing (siRNA- or shRNA-mediated knock-down); CRISPR/Cas-mediated gene editing; cell toxicity assays Microscopy: epifluorescence

complexity arises from regulation and does not correlate with the number of genes. The lab focuses on the regulation of gene expression and RNA biology. In terms of viruses we dissect the regulation of [...] causing various human diseases. Both areas follow our central idea: A deeper knowledge of both viral gene expression and the pathomechanisms of non-coding mutations will advance our understanding of cellular [...] medal: Noncoding mutations reveal new pathological mechanisms and insights into the regulation of gene expression. Wiley Interdiscip Rev RNA. 2021 Jan;12(1):e1616. Review. 2. Krooss S, Werwitzke S, Kopp

Hirntumorfamilien nachzuweisen, dass bestimmte Gene eine Rolle beim Hirntumorrisiko spielen, wenn in der Keimbahn seltene Varianten vorliegen, so die Gene ADAR und RNASEH2B (Beyer & Brand et al., Acta [...] Leukodystrophie, assoziiert sind, das CDH1 Gen (Förster & Brand et al., Acta Neuropathol, 2021), das mit dem hereditären diffusen Magenkarzinom assoziiert ist, sowie die Gene POLE und POLD1 (Weber et al., Acta [...] Keimbahnvarianten in den Genen FIG4 (Osmanovic & Rangnau et al., Eur J Hum Genet, 2017), SPG7 (Osmanovic & Widjaja et al., J Neurol, 2020), DHTKD1 (Osmanovic & Gogol et al., Genes, 2022) und SUMO4 (Osmanovic

Genome-Wide Methylation Mapping Using Nanopore Sequencing Technology Identifies Novel Tumor Suppressor Genes in Hepatocellular Carcinoma. Int. J. Mol. Sci. 2021 , 22 , 3937. doi.org/10.3390/ijms22083937 Sebastian [...] antisense noncoding RNA, EVA1A-AS, suppresses the expression of Myc/Max dependent anti-proliferating gene EVA1A in a U2 dependent manner. Scientific Reports 9, 17319, doi:10.1038/s41598-019-53944-2 (2019) [...] (*equal contribution) Tran DDH* , Kessler C *, Niehus SE, Mahnkopf M, Koch A, Tamura T . Myc target gene, long intergenic noncoding RNA, Linc00176 in hepatocellular carcinoma regulates cell cycle and cell

the European Society of Gene and Cell Therapy (ESGCT) 4th Annual Congress of the German Society for Stem Cell Research (GSZ) 16th Annual meeting of the German Society for Gene Therapy (DG-GT) 2nd Meeting [...] Cell Clonality and Genotoxicity Retreat (SCCGR) Die gemeinsame Jahrestagung der „European Society of Gene and Cell Therapy (ESGCT)“, der “Deutschen Gesellschaft für Stammzellforschung (GSZ)“, der „Deutschen

Bikarbonat durch die epitheliale Apikalmembran transportiert. Die CF auslösenden Mutationen im CFTR Gen führen entweder zum kompletten Funktionsverlust (Mutations-Klasse I), verminderter Synthese von CFTR [...] der CF abschwächen oder sogar kompensieren. Zwar sind mehr als 2000 CF auslösende Mutationen im CFTR Gen bekannt, aber die häufigste Mutation p.Phe508del kommt auf 70% aller CF Chromosomen vor. p.Phe508del [...] können, welche Umweltfaktoren und welche genetischen Faktoren außerhalb des ursächlich betroffenen Gens die Schwere des Krankheitsbilds bei einer angeborenen Erkrankung modulieren. Für die Untersuchungen

increasing the expression of a target gene, while loss-of-function approaches aim to inhibit or silence specific target molecules. For the modulation of targeted gene expression, various strategies are available [...] ated viruses (AAV) are commonly used for gene delivery both in vivo and in vitro . These viral systems allow for the overexpression or silencing of target gene sequences when combined with oligonucleotides [...] promising candidates for treating various diseases. These therapies harness the power of RNA to modulate gene expression or influence epigenetic, posttranscriptional, and translational pathways. Two primary

Award for her impressive work. Annual Meeting of the American Society for Gene and Cell Therapy (ASGCT) The American Society for Gene and Cell Therapy (ASGCT) awards Dr. Mania Ackermann and Ariane Nguyen with [...] presentation at the German Stem Cell Network (GSCN) to present his current work. European Society for Gene and Cell Therapy (ESGCT) 2022 Fawaz Saleh was invited by the ESGCT for a poster presentation of his [...] “Deutsche Gesellschaft für Pneumologie”. ESGCT Collaborative Virtual Congress The European Society of Gene and Cell Therapy (ESGCT) selected Claudio Rodriguez Gonzalez and Shifaa Abdin for oral presentations

poorly understood regulatory network governing gene expression at the translation level. We hypothesize that viral uORFs allow these viruses to adapt viral gene expression to cell type, stress and inflammation [...] herpesviruses encode hundreds of viral proteins and peptides, many of which not only regulate a single gene, DNA or protein but interfere with complex cellular signal networks. A systems-level approach is required [...] the host’s transcriptional machinery to express their large DNA genomes. This process of cellular gene expression is subject to rapid alterations induced by both viral and antiviral mechanisms during lytic

Millionen Euro gefördert. „Die Krankheit wird durch Mutation im sogenannten SMN1-Gen hervorgerufen“, erklärt Professor Claus. Ist das Gen verändert oder geht es völlig verloren, fehlt dem Körper der Bauplan für [...] spielt. Zwar gibt es mit SMN2 noch ein zweites Gen, um das für die Muskelfunktion so wichtige Protein herzustellen. Allerdings sind viele Kopien dieses Gens nötig, um den Verlust von SMN1 auszugleichen.