Therapie, Frankfurt am Main Regensburger Centrum für Interventionelle Immunologie (RCI), Lehrstuhl für Gen-Immuntherapie, Universitätsklinikum Regensburg Miltenyi Biotec B.V. & Co. KG AFFIMED European Commission [...] Commission funded consortium: H2020-MSCA-ITN-MATURE-NK Cluster for Future: Saxocell “Cell and gene therapy”

past years, important advances have been achieved in the field (viral vector-mediated gene transfer, CRISPR cas9 gene editing etc) making these technologies rapid, efficient and more affordable. These methods [...] Ex vivo technologies Genetic engineering is the manipulation of an organism’s genes using biotech methodologies and it includes several applications that are widely adopted to study cardiac biology. In [...] sudden cardiac death or heart failure. Most patients with a confirmed mutation carry mutations in genes encoding sarcomeric proteins in β-myosin heavy chain ( MYH7 , β-MyHC), cardiac myosin-binding protein

internalization and regulates gene expression. Cellular Signalling 26 : 49-55. Hansen, B., Dittrich-Breiholz, O., Kracht, M., and Windheim, M. (2013) Regulation of the NF-kB-dependent gene expression by ligand-induced [...] endent mechanism. Journal of Immunology 201: 3373-3382. Windheim, M. (2016) Interleukin-1-induced gene expression requires the membrane-raft-dependent internalization of the interleukin-1 receptor. Cellular

rases and Related Genes 1035-1045. Bakker, H. and Ashikov, A. (2014). UDP-Xylose and UDP-N-Acetylglucosamine Transporter (SLC35B4). Handbook of Glycosyltransferases and Related Genes 1393-1402. Bakker [...] and Related Genes 1439-1448. Bakker, H. , Ashikov, A., Routier, F.H., and Gerardy-Schahn, R. (2014). GDP-Fucose Transporter 1 (SLC35C1). Handbook of Glycosyltransferases and Related Genes 1403-1411. Buettner [...] A.C., van Zandbergen,G., Wenzel,A., Gerardy-Schahn,R., Wiese,M., and Routier,F.H. (2007). Targeted gene deletion of Leishmania major UDP-galactopyranose mutase leads to attenuated virulence. J. Biol. Chem

Rivera-Pomar R., and Gossler A. (2004). The mouse homeobox gene Not is required for caudal notochord development and affected by the truncate mutation. Genes & Development , 18, 1725-36. Hofmann M., Schuster-Gossler [...] K., Alten, L., Ueffing, M., Blum, M., Gossler, A., 2018. The evolutionary conserved FOXJ1 target gene Fam183b is essential for motile cilia in Xenopus but dispensable for ciliary function in mice. Sci [...] K., Kühnel, M.P., Hegermann, J., Ueffing, M., Gossler, A., 2017. 1700012B09Rik, a FOXJ1 effector gene active in ciliated tissues of the mouse but not essential for motile ciliogenesis. Dev Biol . doi:10

en unter normalen und entzünd­li­chen Bedin­gun­gen und erarbeiten neuartige Isolierungs- und Kultivierungsstrategien. Inhaltliche Schwerpunkte lie­gen auf dem Verständnis von Stammzellfunk­tionen und [...] n und mehrfach gra­dierten Im­plantats für einen zu­künfti­gen Ein­satz am Seh­nen-Knochen-Übergang der Rota­toren­man­schette aufzu­zei­gen. Die FOR 2180 um­spannt Fach­gebiete aus den Ingenieur-, Na­tur- [...] dere auch immun­suppressive) Funk­tionen. Diese Eigenschaften beruhen im we­sentlichen auf Wirkun­gen von lösli­chen Faktoren, die von MSCs abgegeben werden. MSCs sind daher medi­zinisch hoch relevant:

and gene regulation Flow of genetic information The cytoskeleton Microscopic techniques Membrane transport of small molecules Receptors and signal transduction Cell cycle and oncogenesis Human Gene finding [...] lymphoid organs Gene therapy : Stem cells, regulations of transgenes in somatic cells, embryonic and somatic cloning in mammals, Safety and efficiency in the use of viral vectors for gene therapy Microbiology [...] Scientific English 2.5 Radiation protection 2.7 Gene technological security 2.8 Introduction to ethics Ethical cases in medicine Ethical aspects of gene technology Scientific ethics 3. Other Courses 3

Mutationen in mehr als 60 Genen können zu einer Proteinurie führen. Mittels genetischer Analysen (Whole-Genom-Sequenzierung (WGS)) suchen wir nach Mutationen in bisher unbekannten Genen. Ziel ist es durch die [...] Podozyten bilden mit ihren Fußfortsätzen die Schlitzmembran. Insbesondere Mutationen in podozytären Genen können zu Störungen der Filtrationsbarriere führen. Klinisch äußert sich dies durch vermehrten Ei [...] die Charakterisierung der Gene in Zellkultur und Modellorganismen molekulare Signalwege zu identifizieren die neue Therapeutische Angriffspunkte ermöglichen. Wir richten dabei einen besonderen Fokus auf

protein production (E. coli, D. discoideum, Sf9-cells); Fluorescence Labeling techniques; gene silencing; stable gene expression; polyclonal antibody production. Functional Assays: Determination and chara

Western blot, ELISA and further gene expression analyses and is correlated with psychometric data. Thereby establishing the correlations between the regulation of relevant genes and the expression of psych [...] ation of DNA samples. For the analysis of methylation patterns in the promoter region of certain genes, bisulfite sequencing (according to Sanger) has been used in the last 10 years. Recently, nanopore [...] S1 laboratory are equipped for genetic engineering work at safety level 1. CRISPR/Cas9 technology, gene expression and promoter activity studies using the GloMax Multi Detection System from Promega and