Society of Gene and Cell Therapy. Ali RR, Büning H. Hum Gene Ther 2017;28(11):939 Twenty-Five Years of Gene Therapy: Prof. George Dickson on Past Progress and Future Promise. Büning H. Hum Gene Ther 201 [...] A, Schambach A, Henriksen K, Richter J. Hum Gene Ther 2017:DOI:10.1089/hum.2017.053 Gene and Cell Therapy in Germany. von Kalle C, Fehse B, Büning H. Hum Gene Ther 2017;28(10):781 Scavenger receptor class [...] 30. Gene Insertion Into Genomic Safe Harbors for Human Gene Therapy. Papapetrou EP, Schambach A. Mol Ther. 2016 Apr;24(4):678-84. doi: 10.1038/mt.2016.38. Review. Retroviral Vectors for Cancer Gene Therapy

Controlled Switching Between Constitutive Single Gene Expression and Tetracycline-Regulated Multiple Gene Collaboration. Stahlhut M, Schambach A, Kustikova OS. Hum Gene Ther Methods 2017;28(4):191-204 Eliminating [...] in gene therapy. Rothe M, Modlich U, Schambach A. Curr Gene Ther. 2013 Dec;13(6):453-68. Review. Biosafety features of lentiviral vectors. Schambach A, Zychlinski D, Ehrnstroem B, Baum C. Hum Gene Ther [...] Pavlou M, Büning H, Michalakis S, Karl MO. Hum Gene Ther 2021;32(13-14):694-706 DOI: 10.1089/hum.2020.321 Capsid-Engineering for Central Nervous System-Directed Gene Therapy with Adeno-Associated Virus Vectors

the human genome project the number of protein-coding genes has been gradually corrected down to ~20,000 genes, or roughly the same number of genes found in the roundworm C. elegans. During the past decade [...] generation, culture and cardiomyocyte differentiation AAV-mediated gene expression (gene therapy) hypoxia stimulation in vitro antisense-mediated gene and ncRNA knockdown in vitro and in vivo imaging (fluorescence

en zu DNA-Methylierungsmustern von microRNA-Genen in gutartigen Leber-Tumoren 2010 Albat, Cord (Dr. rer.nat.): Aberrante DNA-Methylierung von microRNA-Genen in präneoplastischen Leberzellschädigungen und [...] nalyse differenzierungsassoziierter Gene und vergleichende morphologische Untersuchungen Loch, Gero Hendrik (Dr. med.): Expressionsanalyse fibrose-assoziierter Gene in Knochenmarkzellen der Chronischen [...] entinnen Salim, Mustafa (Dr. med.): Distribution, frequency and function of mutations in the TET2 gene in myeloid neoplasms Schaumann, Nora Mareen (Dr. med.): Untersuchungen zur Interobserver-Konkordanz

n multipler Gene bzw. des gesamten Exoms oder Genoms) gefolgt von detaillierter Datenanalyse mit verschiedenen Analyse- und Filterstrategien. Außerdem charakterisieren wir ausgewählte Gene bzw. genetische [...] neue krankheitsrelevante Gene zu identifizieren, die bei Hirntumoren verändert sind oder der Hirntumorentstehung zugrunde liegen. Weiterhin sind wir auf der Suche nach neuen Genen, deren Veränderungen andere [...] Hydro-/Megaureter, subsumiert. Bisher sind etwa 30 Gene bekannt, deren Veränderungen CAKUT verursachen können. Da eine Mutationsanalyse der bekannten Gene bei CAKUT Patienten häufig nicht erfolgreich ist

Science Bio Informatics CRISPR/Cas9 gene editing in primary T cells Digital Fluorescence microscopy techniques, confocal microscopy and cell transfection Flow-cytometry Gene technology Gnotobiology and rederivation [...] capture of transcribed sequences [SCOTS] Proteome analysis, digital fluorescence microscopy Retroviral gene transfer Single cell immunophenotyping by ChipCytometry Software tools for imaging in infection S

Suicide Gens ( iCASP9 ) in den AAVS1 Safe Harbour Genort die Apoptose der Zellen in vitro und in vivo effizient auslöst, jedoch erst die biallele Integration von zwei Kopien eines Suizid Gens die Entstehung [...] activators or repressors allowing for transcriptional regulation of distinct genes. Although non-viral delivery systems for gene targeting in human iPSCs are quite efficient, viral delivery systems have clear [...] endothelial cells. Besides its safety, SeV can accommodate and express foreign genes, which led to its application as gene therapy vector in clinical trials and as commercial vector for induction of pl

repair in somatic stem cells. A major challenge of gene therapy is insertional transformation of hematopoietic stem cells due to up- or down-regulation of genes critical for hematopoiesis. In collaboration with [...] competitive fitness of normal and malignant hematopoietic stem cells, 3. the development of novel cell and gene therapeutics for inherited or acquired disorders, based on molecular insights into mechanisms of [...] therapeutics. Research at the Institute of Experimental Hematology (IEH) The ongoing improvement of gene transfer technologies for hematopoietic and other cell types has a great impact for the application

mammalian cell culture; transfection; stable and transient gene expression; gene silencing (siRNA- or shRNA-mediated knock-down); CRISPR/Cas-mediated gene editing; cell toxicity assays Microscopy: epifluorescence

cal manipulations in the mouse as well as transgenics and targeted gene disruption to gain information on the mode of action of genes in vivo and in vitro. [...] signaling pathways (including Hedgehog, Wnt, Bmp and Pdgf) and transcription factors of the T-box (Tbx) gene family as well as their integration in patterning and differentiation during organogenesis. We are