Publikationen

Publikationen LEBAO

in press

 

2021

 

Andrade, A. C., Wolf, M., Binder, H. M., Gomes, F. G., Manstein, F., Ebner-Peking, P., Poupardin, R., Zweigerdt, R., Schallmoser, K., and Strunk, D. 2021. Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles. Int J Mol Sci 22, no. 8.

https://doi.org/10.3390/ijms22083890.

 

Banerjee, S., Szepes, M., Dibbert, N., Rios-Camacho, J. C., Kirschning, A., Gruh, I., and Dräger, G. 2021. Dextran-based scaffolds for in-situ hydrogelation: Use for next generation of bioartificial cardiac tissues. Carbohydr Polym 262:117924.

https://doi.org/10.1016/j.carbpol.2021.117924.

 

Benito-Kwiecinski, S.*, Giandomenico, S. L.*, Sutcliffe, M., Riis, E. S., Freire-Pritchett, P., Kelava, I., Wunderlich, S., Martin, U., Wray, G. A., McDole, K., and Lancaster, M. A. 2021. An early cell shape transition drives evolutionary expansion of the human forebrain. Cell 184, no. 8:2084. * authors contributed equally.

https://doi.org/10.1016/j.cell.2021.02.050.

 

Chatterjee, S., Hofer, T., Costa, A., Lu, D., Batkai, S., Gupta, S. K., Bolesani, E., Zweigerdt, R., Megias, D., Streckfuss-Bömeke, K., Brandenberger, C., Thum, T., and Bär, C. 2021. Telomerase therapy attenuates cardiotoxic effects of doxorubicin. Mol Ther 29, no. 4:1395.

https://doi.org/10.1016/j.ymthe.2020.12.035.

 

Drakhlis, L., Biswanath, S., Farr, C. M., Lupanow, V., Teske, J., Ritzenhoff, K., Franke, A., Manstein, F., Bolesani, E., Kempf, H., Liebscher, S., Schenke-Layland, K., Hegermann, J., Nolte, L., Meyer, H., de la Roche, J., Thiemann, S., Wahl-Schott, C., Martin, U., and Zweigerdt, R. 2021. Human heart-forming organoids recapitulate early heart and foregut development. Nat Biotechnol 39, no. 6:737.

https://doi.org/10.1038/s41587-021-00815-9.

 

Drakhlis, L.*, Devadas, S. B.*, and Zweigerdt, R. 2021. Generation of heart-forming organoids from human pluripotent stem cells. Nat Protoc. * authors contributed equally.

https://doi.org/10.1038/s41596-021-00629-8.

 

Ebken, J., Mester, N., Smart, I., Ramm, R., Goecke, T., Jashari, R., Böthig, D., Horke, A., Cebotari, S., Tudorache, I., Avsar, M., Bobylev, D., Haverich, A., Sarikouch, S., and Hilfiker, A. 2021. Residual immune response towards decellularized homografts may be highly individual. Eur J Cardiothorac Surg 59, no. 4:773.

https://doi.org/10.1093/ejcts/ezaa393.

 

Eggenschwiler, R., Gschwendtberger, T., Felski, C., Jahn, C., Langer, F., Sterneckert, J., Hermann, A., Lühmann, J., Steinemann, D., Haase, A., Martin, U., Petri, S., and Cantz, T. 2021. A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines. Sci Rep 11, no. 1:22154.

https://doi.org/10.1038/s41598-021-01689-2.

 

Eyre, K., Samper, E., Haverich, A., Hilfiker, A., and Andrée, B. 2021. Re-endothelialization of non-detergent decellularized porcine vessels. Artif Organs 45, no. 4:E53.

https://doi.org/10.1111/aor.13836.

 

Haase, A., Kohrn, T., Fricke, V., Ricci Signorini, M. E., Witte, M., Göhring, G., Gruh, I., and Martin, U. 2021. Establishment of MHHi001-A-5, a GCaMP6f and RedStar dual reporter human iPSC line for in vitro and in vivo characterization and in situ tracing of iPSC derivatives. Stem Cell Res 52:102206.

https://doi.org/10.1016/j.scr.2021.102206.

 

Kosanke, M., Davenport, C., Szepes, M., Wiehlmann, L., Kohrn, T., Dorda, M., Gruber, J., Menge, K., Sievert, M., Melchert, A., Gruh, I., Göhring, G., and Martin, U. 2021. iPSC culture expansion selects against putatively actionable mutations in the mitochondrial genome. Stem Cell Reports 16, no. 10:2488.

https://doi.org/10.1016/j.stemcr.2021.08.016.

 

Kosanke, M., Osetek, K., Haase, A., Wiehlmann, L., Davenport, C., Schwarzer, A., Adams, F., Kleppa, M. J., Schambach, A., Merkert, S., Wunderlich, S., Menke, S., Dorda, M., and Martin, U. 2021. Reprogramming enriches for somatic cell clones with small-scale mutations in cancer-associated genes. Mol Ther.

https://doi.org/10.1016/j.ymthe.2021.04.007.

 

Liu, D., Caliskan, S., Rashidfarokhi, B., Oldenhof, H., Jung, K., Sieme, H., Hilfiker, A., and Wolkers, W. F. 2021. Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves. Sci Rep 11, no. 1:12299.

https://doi.org/10.1038/s41598-021-91802-2.

 

Malik, M. N. H., Waqas, S. F. H., Zeitvogel, J., Cheng, J., Geffers, R., Gouda, Z. A., Elsaman, A. M., Radwan, A. R., Schefzyk, M., Braubach, P., Auber, B., Olmer, R., Müsken, M., Roesner, L. M., Gerold, G., Schuchardt, S., Merkert, S., Martin, U., Meissner, F., Werfel, T., and Pessler, F. 2021. Congenital deficiency reveals critical role of ISG15 in skin homeostasis. J Clin Invest.

https://doi.org/10.1172/jci141573.

 

Manstein, F., Ullmann, K., Kropp, C., Halloin, C., Triebert, W., Franke, A., Farr, C. M., Sahabian, A., Haase, A., Breitkreuz, Y., Peitz, M., Brüstle, O., Kalies, S., Martin, U., Olmer, R., and Zweigerdt, R. 2021. High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling. Stem Cells Transl Med.

https://doi.org/10.1002/sctm.20-0453.

 

Merkert, S., Wunderlich, S., Beier, J., Franke, A., Schwanke, K., Göhring, G., Kraft, T., Francino, A., Zweigerdt, R., and Martin, U. 2021. Generation of two iPSC clones (MHHi021-A and MHHi021-B) from a patient with hypertrophic cardiomyopathy with p.Arg723Gly mutation in the MYH7 gene. Stem Cell Res 52:102208.

https://doi.org/10.1016/j.scr.2021.102208.

 

Morticelli, L., Magdei, M., Tschalaki, N., Petersen, B., Haverich, A., and Hilfiker, A. 2021. Generation of glycans depleted decellularized porcine pericardium, using digestive enzymatic supplements and enzymatic mixtures for food industry. Xenotransplantation:e12705.

https://doi.org/10.1111/xen.12705.

 

Pflaum, M., Dahlmann, J., Engels, L., Naghilouy-Hidaji, H., Adam, D., Zöllner, J., Otto, A., Schmeckebier, S., Martin, U., Haverich, A., Olmer, R., and Wiegmann, B. 2021. Towards Biohybrid Lung: Induced Pluripotent Stem Cell Derived Endothelial Cells as Clinically Relevant Cell Source for Biologization. Micromachines (Basel) 12, no. 8.

https://doi.org/10.3390/mi12080981.

 

Polyak, A., Bankstahl, J. P., Besecke, K. F. W., Hozsa, C., Triebert, W., Pannem, R. R., Manstein, F., Borcholte, T., Furch, M., Zweigerdt, R., Gieseler, R. K., Bengel, F. M., and Ross, T. L. 2021. Simplified (89)Zr-Labeling Protocol of Oxine (8-Hydroxyquinoline) Enabling Prolonged Tracking of Liposome-Based Nanomedicines and Cells. Pharmaceutics 13, no. 7.

https://doi.org/10.3390/pharmaceutics13071097.

 

Rafiei Hashtchin, A., Fehlhaber, B., Hetzel, M., Manstein, F., Stalp, J. L., Glage, S., Abeln, M., Zweigerdt, R., Munder, A., Viemann, D., Ackermann, M., and Lachmann, N. 2021. Human iPSC-derived macrophages for efficient Staphylococcus aureus clearance in a murine pulmonary infection model. Blood Adv.

https://doi.org/10.1182/bloodadvances.2021004853.

 

Ricke-Hoch, M., Stelling, E., Lasswitz, L., Gunesch, A. P., Kasten, M., Zapatero-Belinchón, F. J., Brogden, G., Gerold, G., Pietschmann, T., Montiel, V., Balligand, J. L., Facciotti, F., Hirsch, E., Gausepohl, T., Elbahesh, H., Rimmelzwaan, G. F., Höfer, A., Kühnel, M. P., Jonigk, D., Eigendorf, J., Tegtbur, U., Mink, L., Scherr, M., Illig, T., Schambach, A., Pfeffer, T. J., Hilfiker, A., Haverich, A., and Hilfiker-Kleiner, D. 2021. Impaired immune response mediated by prostaglandin E2 promotes severe COVID-19 disease. PLoS One 16, no. 8:e0255335.

https://doi.org/10.1371/journal.pone.0255335.

 

Sahabian, A.*, Dahlmann, J.*, Martin, U.#, and Olmer, R.#. 2021. Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions. Nat Protoc. */# authors contributed equally.

https://doi.org/10.1038/s41596-020-00470-5.

 

Smart, I., Goecke, T., Ramm, R., Petersen, B., Lenz, D., Haverich, A., Niemann, H., and Hilfiker, A. 2021. Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves. Xenotransplantation 28, no. 1:e12646.

https://doi.org/10.1111/xen.12646.

 

Wolkers, W. F., and Hilfiker, A. 2021. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability. Methods Mol Biol 2180:731.

https://doi.org/10.1007/978-1-0716-0783-1_40.

 

2020

 

Dahlmann, J., Sahabian, A., Drick, N., Haase, A., Göhring, G., Lachmann, N., Ringshausen, F. C., Welte, T., Martin, U., and Olmer, R. 2020. Generation of two hiPSC lines (MHHi016-A, MHHi016-B) from a primary ciliary dyskinesia patient carrying a homozygous 5 bp duplication (c.248_252dup (p.Gly85Cysfs*11)) in exon 1 of the CCNO gene. Stem Cell Res 46:101850.

https://doi.org/10.1016/j.scr.2020.101850.

 

Drick, N., Dahlmann, J., Sahabian, A., Haase, A., Göhring, G., Lachmann, N., Ringshausen, F. C., Welte, T., Martin, U., and Olmer, R. 2020. Generation of two human induced pluripotent stem cell lines (MHHi017-A, MHHi017-B) from a patient with primary ciliary dyskinesia carrying a homozygous mutation (c.7915C > T [p.Arg2639*]) in the DNAH5 gene. Stem Cell Res 46:101848.

https://doi.org/10.1016/j.scr.2020.101848.

 

Drick, N., Sahabian, A., Pongpamorn, P., Merkert, S., Göhring, G., Welte, T., Martin, U., and Olmer, R. 2020. Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4). Stem Cell Res 42:101659.

https://doi.org/10.1016/j.scr.2019.101659.

 

Findeisen, K., Morticelli, L., Goecke, T., Kolbeck, L., Ramm, R., Höffler, H. K., Brandes, G., Korossis, S., Haverich, A., and Hilfiker, A. 2020. Toward acellular xenogeneic heart valve prostheses: Histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices. Xenotransplantation 27, no. 5:e12617.

https://doi.org/10.1111/xen.12617.

 

Godehardt, A. W., Ramm, R., Gulich, B., Tönjes, R. R., and Hilfiker, A. 2020. Decellularized pig pulmonary heart valves-Depletion of nucleic acids measured by proviral PERV pol. Xenotransplantation 27, no. 2:e12565.

https://doi.org/10.1111/xen.12565.

 

Hunkler, H. J., Hoepfner, J., Huang, C. K., Chatterjee, S., Jara-Avaca, M., Gruh, I., Bolesani, E., Zweigerdt, R., Thum, T., and Bär, C. 2020. The Long Non-coding RNA Cyrano Is Dispensable for Pluripotency of Murine and Human Pluripotent Stem Cells. Stem Cell Reports 15, no. 1:13.

https://doi.org/10.1016/j.stemcr.2020.05.011.

 

Ichanti, H., Sladic, S., Kalies, S., Haverich, A., Andrée, B., and Hilfiker, A. 2020. Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels. Gels 6, no. 3.

https://doi.org/10.3390/gels6030027.

 

Kreuser, U., Buchert, J., Haase, A., Richter, W., and Diederichs, S. 2020. Initial WNT/β-Catenin Activation Enhanced Mesoderm Commitment, Extracellular Matrix Expression, Cell Aggregation and Cartilage Tissue Yield From Induced Pluripotent Stem Cells. Front Cell Dev Biol 8:581331.

https://doi.org/10.3389/fcell.2020.581331.

 

Lipus, A.*, Janosz, E.*, Ackermann, M., Hetzel, M., Dahlke, J., Buchegger, T., Wunderlich, S., Martin, U., Cathomen, T., Schambach, A., Moritz, T.#, and Lachmann, N.#. 2020. Targeted Integration of Inducible Caspase-9 in Human iPSCs Allows Efficient in vitro Clearance of iPSCs and iPSC-Macrophages. Int J Mol Sci 21, no. 7. */# authors contributed equally.

https://doi.org/10.3390/ijms21072481.

 

Merkert, S., Jaboreck, M. C., Engels, L., Malik, M. N. H., Göhring, G., Pessler, F., Martin, U., and Olmer, R. 2020. Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing. Stem Cell Res 50:102135

https://doi.org/10.1016/j.scr.2020.102135.

 

Merkert, S., Schubert, M., Haase, A., Janssens, H. M., Scholte, B., Lachmann, N., Gohring, G., and Martin, U. 2020. Generation of an induced pluripotent stem cell line (MHHi018-A) from a patient with Cystic Fibrosis carrying p.Asn1303Lys (N1303K) mutation. Stem Cell Res 44:101744.

https://doi.org/10.1016/j.scr.2020.101744.

 

Nowak-Imialek, M., Wunderlich, S., Herrmann, D., Breitschuh-Leibling, S., Gohring, G., Petersen, B., Klein, S., Baulain, U., Lucas-Hahn, A., Martin, U., and Niemann, H. 2020. In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos. Cell Reprogram 22, no. 3:118.

https://doi.org/10.1089/cell.2019.0107.

 

Pongpamorn, P.*, Dahlmann, J.*, Haase, A., Ebeling, C. T., Merkert, S., Gohring, G., Lachmann, N., Martens, A., Haverich, A., Martin, U.#, and Olmer, R. #. 2020. Generation of three induced pluripotent stem cell lines (MHHi012-A, MHHi013-A, MHHi014-A) from a family with Loeys-Dietz syndrome carrying a heterozygous p.M253I (c.759G>A) mutation in the TGFBR1 gene. Stem Cell Res 43:101707. */# authors contributed equally.

https://doi.org/10.1016/j.scr.2020.101707.

 

Ramm, R., Goecke, T., Theodoridis, K., Hoeffler, K., Sarikouch, S., Findeisen, K., Ciubotaru, A., Cebotari, S., Tudorache, I., Haverich, A., and Hilfiker, A. 2020. Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model. Xenotransplantation 27, no. 2:e12571.

https://doi.org/10.1111/xen.12571.

 

Sahabian, A., von Schlehdorn, L., Drick, N., Pink, I., Dahlmann, J., Haase, A., Göhring, G., Welte, T., Martin, U., Ringshausen, F. C., and Olmer, R. 2020. Generation of two hiPSC clones (MHHi019-A, MHHi019-B) from a primary ciliary dyskinesia patient carrying a homozygous deletion in the NME5 gene (c.415delA (p.Ile139Tyrfs*8)). Stem Cell Res 48:101988.

https://doi.org/10.1016/j.scr.2020.101988.

 

Stelling, E., Ricke-Hoch, M., Erschow, S., Hoffmann, S., Bergmann, A. K., Heimerl, M., Pietzsch, S., Battmer, K., Haase, A., Stapel, B., Scherr, M., Balligand, J. L., Binah, O., and Hilfiker-Kleiner, D. 2020. Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation. PLoS Biol 18, no. 12:e3000739.

https://doi.org/10.1371/journal.pbio.3000739.

 

Szepes, M., Melchert, A., Dahlmann, J., Hegermann, J., Werlein, C., Jonigk, D., Haverich, A., Martin, U., Olmer, R., and Gruh, I. 2020. Dual Function of iPSC-Derived Pericyte-Like Cells in Vascularization and Fibrosis-Related Cardiac Tissue Remodeling In Vitro. Int J Mol Sci 21, no. 23.

https://doi.org/10.3390/ijms21238947.

 

Taghizadeh, S., Jones, M. R., Olmer, R., Ulrich, S., Danopoulos, S., Shen, C., Chen, C., Wilhelm, J., Martin, U., Chen, C., Al Alam, D., and Bellusci, S. 2020. Fgf10 Signaling-Based Evidence for the Existence of an Embryonic Stage Distinct From the Pseudoglandular Stage During Mouse Lung Development. Front Cell Dev Biol 8:576604.

https://doi.org/10.3389/fcell.2020.576604.

 

Weber, N.*, Kowalski, K.*, Holler, T., Radocaj, A., Fischer, M., Thiemann, S., de la Roche, J., Schwanke, K., Piep, B., Peschel, N., Krumm, U., Lingk, A., Wendland, M., Greten, S., Schmitto, J. D., Ismail, I., Warnecke, G., Zywietz, U., Chichkov, B., Meissner, J., Haverich, A., Martin, U., Brenner, B., Zweigerdt, R., and Kraft, T. 2020. Advanced Single-Cell Mapping Reveals that in hESC Cardiomyocytes Contraction Kinetics and Action Potential Are Independent of Myosin Isoform. Stem Cell Reports. * authors contributed equaly.

https://doi.org/10.1016/j.stemcr.2020.03.015.

 

Williams, B., Löbel, W., Finklea, F., Halloin, C., Ritzenhoff, K., Manstein, F., Mohammadi, S., Hashemi, M., Zweigerdt, R., Lipke, E., and Cremaschi, S. 2020. Prediction of Human Induced Pluripotent Stem Cell Cardiac Differentiation Outcome by Multifactorial Process Modeling. Front Bioeng Biotechnol 8:851.

https://doi.org/10.3389/fbioe.2020.00851.