Hematopoiesis

Further publications:   Gene Therapy     Vectorology / Biosafety     Regenerative Medicine

2019

2018

• CRISPR/Cas9 Immunoengineering of Hoxb8-Immortalized Progenitor Cells for Revealing CCR7-Mediated Dendritic Cell Signaling and Migration Mechanisms in vivo.
  Hammerschmidt SI, Werth K, Rothe M, Galla M, Permanyer M, Patzer GE, Bubke A, Frenk DN, Selich A, Lange L, Schambach A, Bošnjak B, Förster R.
  Front Immunol. 2018 Aug 28;9:1949. doi: 10.3389/fimmu.2018.01949.

• Endogenous Tumor Suppressor microRNA-193b: Therapeutic and Prognostic Value in Acute Myeloid Leukemia.
  Bhayadia R, Krowiorz K, Haetscher N, Jammal R, Emmrich S, Obulkasim A, Fiedler J, Schwarzer A, Rouhi A, Heuser M, Wingert S, Bothur S, Döhner K, Mätzig T, Ng M, Reinhardt D, Döhner H, Zwaan CM, van den Heuvel Eibrink M, Heckl D, Fornerod M, Thum T, Humphries RK, Rieger MA, Kuchenbauer F, Klusmann JH.
  J Clin Oncol. 2018 Apr 1;36(10):1007-1016. doi: 10.1200/JCO.2017.75.2204.

• EVI1 carboxy-terminal phosphorylation is ATM-mediated and sustains transcriptional modulation and self-renewal via enhanced CtBP1 association.
  Paredes R, Schneider M, Stevens A, White DJ, Williamson AJK, Muter J, Pearson S, Kelly JR, Connors K, Wiseman DH, Chadwick JA, Löffler H, Teng HY, Lovell S, Unwin R, van de Vrugt HJ, Smith H, Kustikova O, Schambach A, Somervaille TCP, Pierce A, Whetton AD, Meyer S.
  Nucleic Acids Res. 2018 Sep 6;46(15):7662-7674. doi: 10.1093/nar/gky536.

• Human Teratoma-Derived Hematopoiesis Is a Highly Polyclonal Process Supported by Human Umbilical Vein Endothelial Cells.
  Philipp F, Selich A, Rothe M, Hoffmann D, Rittinghausen S, Morgan MA, Klatt D, Glage S, Lienenklaus S, Neuhaus V, Sewald K, Braun A, Schambach A.
  Stem Cell Reports. 2018 Nov 13;11(5):1051-1060. doi: 10.1016/j.stemcr.2018.09.010.

• Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.
  Ziegler PK, Bollrath J, Pallangyo CK, Matsutani T, Canli Ö, De Oliveira T, Diamanti MA, Müller N, Gamrekelashvili J, Putoczki T, Horst D, Mankan AK, Öner MG, Müller S, Müller-Höcker J, Kirchner T, Slotta-Huspenina J, Taketo MM, Reinheckel T, Dröse S, Larner AC, Wels WS, Ernst M, Greten TF, Arkan MC, Korn T, Wirth D, Greten FR.
  Cell. 2018 Jun 28;174(1):88-101.e16. doi: 10.1016/j.cell.2018.05.028.

• UM171 Enhances Lentiviral Gene Transfer and Recovery of Primitive Human Hematopoietic Cells.
  Ngom M, Imren S, Maetzig T, Adair JE, Knapp DJHF, Chagraoui J, Fares I, Bordeleau ME, Sauvageau G, Leboulch P, Eaves C, Humphries RK.
  Mol Ther Methods Clin Dev. 2018 Jul 5;10:156-164. doi: 10.1016/j.omtm.2018.06.009.

2017

• A four-gene LincRNA expression signature predicts risk in multiple cohorts of acute myeloid leukemia patients.
  Beck D, Thoms JAI, Palu C, Herold T, Shah A, Olivier J, Boelen L, Huang Y, Chacon D, Brown A, Babic M, Hahn C, Perugini M, Zhou X, Huntly BJ, Schwarzer A, Klusmann JH, Berdel WE, Wörmann B, Büchner T, Hiddemann W, Bohlander SK, To LB, Scott HS, Lewis ID, D'Andrea RJ, Wong JWH, Pimanda JE.
  Leukemia 2017:DOI: 10.1038/leu.2017.210
   
• TLR9-Mediated Conditioning of Liver Environment Is Essential for Successful Intrahepatic Immunotherapy and Effective Memory Recall.
  Cebula M, Riehn M, Hillebrand U, Kratzer RF, Kreppel F, Koutsoumpli G, Daemen T, Hauser H, Wirth D.
  Mol Ther 2017;25(10):2289-2298
   
• Hepatic T Cell Tolerance Induction in An Inflammatory Environment.
  Dywicki J, Noyan F, Misslitz AC, Hapke M, Galla M, Schlue J, Liblau RS, Taubert R, Manns MP, Jaeckel E, Hardtke-Wolenski M.
  Dig Dis 2017:DOI: 10.1159/000481341
   
• CSF-1-induced Src signaling can instruct monocytic lineage choice.
  Endele M, Loeffler D, Kokkaliaris KD, Hilsenbeck O, Skylaki S, Hoppe PS, Schambach A, Stanley ER, Schroeder T.
  Blood 2017;129(12):1691-1701
   
• Diet induced obesity has an influence on intrahepatic T cell responses.
  Gaur P, Cebula M, Riehn M, Hillebrand U, Hauser H, Wirth D.
  Metabolism 2017;69:171-176
   
• Meis2 as a critical player in MN1-induced leukemia.
  Lai CK, Norddahl GL, Maetzig T, Rosten P, Lohr T, Sanchez Milde L, von Krosigk N, Docking TR, Heuser M, Karsan A, Humphries RK.
  Blood Cancer J 2017;7(9):e613
   
• A novel AHI-1-BCR-ABL-DNM2 complex regulates leukemic properties of primitive CML cells through enhanced cellular endocytosis and ROS-mediated autophagy.
  Liu X, Rothe K, Yen R, Fruhstorfer C, Maetzig T, Chen M, Forrest DL, Humphries RK, Jiang X.
  Leukemia 2017;31(11):2376-2387
   
• The Guanine-Nucleotide Exchange Factor Caldag Gefi Fine-Tunes Functional Properties of Regulatory T Cells.
  Niemz J, Kliche S, Pils MC, Morrison E, Manns A, Freund C, Crittenden JR, Graybiel AM, Galla M, Jänsch L, Huehn J.
  Eur J Microbiol Immunol (Bp) 2017;7(2):112-126
   
• Mesenteric lymph node stromal cell-derived extracellular vesicles contribute to peripheral de novo induction of Foxp3(+) regulatory T cells.
  Pasztoi M, Pezoldt J, Beckstette M, Lipps C, Wirth D, Rohde M, Paloczi K, Buzas EI, Huehn J.
  Eur J Immunol 2017;47(12):2142-2152
   
• CpG-ODN Facilitates Effective Intratracheal Immunization and Recall of Memory against Neoantigen-Expressing Alveolar Cells.
  Riehn M, Cebula M, Hauser H, Wirth D.
  Front Immunol 2017;8:1201
   
• Uncoupling the Oncogenic Engine.
  Schambach A, Schott JW, Morgan MA.
  Cancer Res 2017;77(22):6060-6064
   
• The non-coding RNA landscape of human hematopoiesis and leukemia.
  Schwarzer A, Emmrich S, Schmidt F, Beck D, Ng M, Reimer C, Adams FF, Grasedieck S, Witte D, Kabler S, Wong JWH, Shah A, Huang Y, Jammal R, Maroz A, Jongen-Lavrencic M, Schambach A, Kuchenbauer F, Pimanda JE, Reinhardt D, Heckl D, Klusmann JH.
  Nat Commun 2017;8(1):218
   
• A knock-in mouse strain facilitates dynamic tracking and enrichment of MEIS1.
  Xiang P, Wei W, Hofs N, Clemans-Gibbon J, Maetzig T, Lai CK, Dhillon I, May C, Ruschmann J, Schneider E, Rosten P, Hu K, Kuchenbauer F, Hoodless PA, Humphries RK.
  Blood Adv 2017;1(24):2225-2235
   
• Forced expression of human M-CSF in CD34+ cells promotes monocyte differentiation in vitro and in vivo but blunts osteoclastogenesis in vitro.
  Montano Almendras CP, Thudium CS, Löfvall H, Moscatelli I, Schambach A, Henriksen K, Richter J.
  Eur J Haematol. 2017 Feb 3. doi: 10.1111/ejh.12867.

• CSF-1-induced Src signaling can instruct monocytic lineage choice.
  Endele M, Loeffler D, Kokkaliaris KD, Hilsenbeck O, Skylaki S, Hoppe PS, Schambach A, Stanley ER, Schroeder T.
  Blood. 2017 Feb 3. pii: blood-2016-05-714329. doi: 10.1182/blood-2016-05-714329.

2016

• Monocyte/macrophage lineage commitment and distribution are affected by the lack of regulatory T cells in scurfy mice.
  Skuljec J, Cabanski M, Surdziel E, Lachmann N, Brennig S, Pul R, Jirmo AC, Habener A, Visic J, Dalüge K, Hennig C, Moritz T, Happle C, Hansen G.
  Eur J Immunol. 2016 Jul;46(7):1656-68. doi: 10.1002/eji.201546200.

• GABP is necessary for stem/progenitor cell maintenance and myeloid differentiation in human hematopoiesis and chronic myeloid leukemia.
  Manukjan G, Ripperger T, Venturini L, Stadler M, Göhring G, Schambach A, Schlegelberger B, Steinemann D.
  Stem Cell Res. 2016 May;16(3):677-81. doi: 10.1016/j.scr.2016.04.007.

• Establishing a murine xenograft-model for long-term analysis of factors inducing chromosomal instability in myelodysplastic syndrome: Pitfalls and successes.
  Salari A, Thomay K, Himmler K, Vajen B, Schienke A, Hagedorn M, Ebersold J, Kreipe HH, Krüger A, Schambach A, Schlegelberger B, Göhring G.
  Cancer Genet. 2016 Jun;209(6):258-66. doi: 10.1016/j.cancergen.2016.04.004.

• Preclinical validation: LV/IL-12 transduction of patient leukemia cells for immunotherapy of AML.
  Huang J, Liu Y, Au BC, Barber DL, Arruda A, Schambach A, Rothe M, Minden MD, Paige CJ, Medin JA.
  Mol Ther Methods Clin Dev. 2016 Dec 7;3:16074.

• Pyrimethamine as a Potent and Selective Inhibitor of Acute Myeloid Leukemia Identified by High-throughput Drug Screening.
  Sharma A, Jyotsana N, Lai CK, Chaturvedi A, Gabdoulline R, Görlich K, Murphy C, Blanchard JE, Ganser A, Brown E, Hassell JA, Humphries RK, Morgan M, Heuser M.
  Curr Cancer Drug Targets. 2016;16(9):818-828.

• Enantiomer-specific and paracrine leukemogenicity of mutant IDH metabolite 2-hydroxyglutarate.
  Chaturvedi A, Araujo Cruz MM, Jyotsana N, Sharma A, Goparaju R, Schwarzer A, Görlich K, Schottmann R, Struys EA, Jansen EE, Rohde C, Müller-Tidow C, Geffers R, Göhring G, Ganser A, Thol F, Heuser M.
  Leukemia. 2016 Aug;30(8):1708-15. doi: 10.1038/leu.2016.71.

2015

• Inhibition of Thrombopoietin/Mpl Signaling in Adult Hematopoiesis Identifies New Candidates for Hematopoietic Stem Cell Maintenance.
  Kohlscheen S, Wintterle S, Schwarzer A, Kamp C, Brugman MH, Breuer DC, Büsche G, Baum C, Modlich U.
  PLoS One. 2015 Jul 6;10(7):e0131866. doi: 10.1371/journal.pone.0131866. eCollection 2015.

• Comparison of Different Cytokine Conditions Reveals Resveratrol as a New Molecule for Ex Vivo Cultivation of Cord Blood-Derived Hematopoietic Stem Cells.
  Heinz N, Ehrnström B, Schambach A, Schwarzer A, Modlich U, Schiedlmeier B.
  Stem Cells Transl Med. 2015 Sep;4(9):1064-72. doi: 10.5966/sctm.2014-0284. Epub 2015 Jul 9.

• Rescue of DNA-PK Signaling and T-Cell Differentiation by Targeted Genome Editing in a prkdc Deficient iPSC Disease Model.
  Rahman SH, Kuehle J, Reimann C, Mlambo T, Alzubi J, Maeder ML, Riedel H, Fisch P, Cantz T, Rudolph C, Mussolino C, Joung JK, Schambach A, Cathomen T.
  PLoS Genet. 2015 May 22;11(5):e1005239. doi: 10.1371/journal.pgen.1005239. eCollection 2015 May.

• Hyperactivation of mTORC1 and mTORC2 by multiple oncogenic events causes addiction to eIF4E-dependent mRNA translation in T-cell leukemia.
  Schwarzer A, Holtmann H, Brugman M, Meyer J, Schauerte C, Zuber J, Steinemann D, Schlegelberger B, Li Z, Baum C.
  Oncogene. 2015 Jul;34(27):3593-604. doi: 10.1038/onc.2014.290. Epub 2014 Sep 22.

2014

• Clonal Dominance With Retroviral Vector Insertions Near the ANGPT1 and ANGPT2 Genes in a Human Xenotransplant Mouse Model.
  Haemmerle R, Phaltane R, Rothe M, Schröder S, Schambach A, Moritz T, Modlich U.
  Mol Ther Nucleic Acids. 2014 Oct 7;3:e200. doi: 10.1038/mtna.2014.51.

• Dose response and clonal variability of lentiviral tetracycline-regulated vectors in murine hematopoietic cells.
  Kustikova OS, Stahlhut M, Ha TC, Scherer R, Schambach A, Baum C.
  Exp Hematol. 2014 Jul;42(7):505-515.e7. doi: 10.1016/j.exphem.2014.03.004. Epub 2014 Mar 24.

• Lack of noncanonical RAS mutations in cytogenetically normal acute myeloid leukemia.
  Reuter CW, Krauter J, Onono FO, Bunke T, Damm F, Thol F, Wagner K, Göhring G, Schlegelberger B, Heuser M, Ganser A, Morgan MA.
  Ann Hematol. 2014 Jun;93(6):977-82. doi: 10.1007/s00277-014-2061-9. Epub 2014 Apr 16.

2013

• Evaluating a ligation-mediated PCR and pyrosequencing method for the detection of clonal contribution in polyclonal retrovirally transduced samples.
  Brugman MH, Suerth JD, Rothe M, Suerbaum S, Schambach A, Modlich U, Kustikova O, Baum C.
  Hum Gene Ther Methods. 2013 Apr;24(2):68-79. doi: 10.1089/hgtb.2012.175. Epub 2013 Mar 14.

• Activation of Evi1 inhibits cell cycle progression and differentiation of hematopoietic progenitor cells.
  Kustikova OS, Schwarzer A, Stahlhut M, Brugman MH, Neumann T, Yang M, Li Z, Schambach A, Heinz N, Gerdes S, Roeder I, Ha TC, Steinemann D, Schlegelberger B, Baum C.
  Leukemia. 2013 Apr;27(5):1127-38. doi: 10.1038/leu.2012.355. Epub 2012 Dec 5.

• NADH dehydrogenase subunit 4 variant sequences in childhood acute myeloid leukaemia.
  Morgan MA, Markus B, Hermkens M, Damm F, Reinhardt D, Zimmermann M, Thol F, Bunke T, Bogoeva D, Reuter CW, de Haas V, van den Heuvel-Eibrink MM, Zwaan CM, Reinhardt K.
  Br J Haematol. 2013 Jun;161(6):891-5. doi: 10.1111/bjh.12298. Epub 2013 Mar 25. No abstract available.

2012

• In vivo enrichment of cytidine deaminase gene-modified hematopoietic cells by prolonged cytosine-arabinoside application.
  Brennig S, Rattmann I, Lachmann N, Schambach A, Williams DA, Moritz T.
  Cytotherapy. 2012 Apr;14(4):451-60. doi: 10.3109/14653249.2011.646043. Epub 2012 Jan 16.

• Serum- and stromal cell-free hypoxic generation of embryonic stem cell-derived hematopoietic cells in vitro, capable of multilineage repopulation of immunocompetent mice.
  Lesinski DA, Heinz N, Pilat-Carotta S, Rudolph C, Jacobs R, Schlegelberger B, Klump H, Schiedlmeier B.
  Stem Cells Transl Med. 2012 Aug;1(8):581-91. doi: 10.5966/sctm.2012-0020. Epub 2012 Aug 6.

2011

• Lentiviral gene transfer regenerates hematopoietic stem cells in a mouse model for Mpl-deficient aplastic anemia.
  Heckl D, Wicke DC, Brugman MH, Meyer J, Schambach A, Büsche G, Ballmaier M, Baum C, Modlich U.
  Blood. 2011 Apr 7;117(14):3737-47. doi: 10.1182/blood-2010-09-308262. Epub 2011 Feb 2.

• Polyclonal fluctuation of lentiviral vector-transduced and expanded murine hematopoietic stem cells.
  Maetzig T, Brugman MH, Bartels S, Heinz N, Kustikova OS, Modlich U, Li Z, Galla M, Schiedlmeier B, Schambach A, Baum C.
  Blood. 2011 Mar 17;117(11):3053-64. doi: 10.1182/blood-2010-08-303222. Epub 2011 Jan 19.

• Leukemias induced by altered TRK-signaling are sensitive to mTOR inhibitors in preclinical models.
  Rhein M, Schwarzer A, Yang M, Kaever V, Brugman M, Meyer J, Ganser A, Baum C, Li Z.
  Ann Hematol. 2011 Mar;90(3):283-92. doi: 10.1007/s00277-010-1065-3. Epub 2010 Sep 7.

2002 - 2010

• Gene therapy of MPL deficiency: challenging balance between leukemia and pancytopenia.
  Wicke DC, Meyer J, Buesche G, Heckl D, Kreipe H, Li Z, Welte KH, Ballmaier M, Baum C, Modlich U.
  Mol Ther. 2010 Feb;18(2):343-52. doi: 10.1038/mt.2009.233. Epub 2009 Oct 20. Erratum in: Mol Ther. 2010 Feb;18(2):448.

• Insertional transformation of hematopoietic cells by self-inactivating lentiviral and gammaretroviral vectors.
  Modlich U, Navarro S, Zychlinski D, Maetzig T, Knoess S, Brugman MH, Schambach A, Charrier S, Galy A, Thrasher AJ, Bueren J, Baum C.
  Mol Ther. 2009 Nov;17(11):1919-28. doi: 10.1038/mt.2009.179. Epub 2009 Aug 11.

• High-affinity neurotrophin receptors and ligands promote leukemogenesis.
  Li Z, Beutel G, Rhein M, Meyer J, Koenecke C, Neumann T, Yang M, Krauter J, von Neuhoff N, Heuser M, Diedrich H, Göhring G, Wilkens L, Schlegelberger B, Ganser A, Baum C.
  Blood. 2009 Feb 26;113(9):2028-37. doi: 10.1182/blood-2008-05-155200.

• Murine hematopoietic stem cell transduction using retroviral vectors.
  Modlich U, Schambach A, Li Z, Schiedlmeier B.
  Methods Mol Biol. 2009;506:23-31. doi: 10.1007/978-1-59745-409-4_3.

• Leukemia induction after a single retroviral vector insertion in Evi1 or Prdm16.
  Modlich U, Schambach A, Brugman MH, Wicke DC, Knoess S, Li Z, Maetzig T, Rudolph C, Schlegelberger B, Baum C.
  Leukemia. 2008 Aug;22(8):1519-28. doi: 10.1038/leu.2008.118. Epub 2008 May 22.

• Remarkable leukemogenic potency and quality of a constitutively active neurotrophin receptor, deltaTrkA.
  Meyer J, Rhein M, Schiedlmeier B, Kustikova O, Rudolph C, Kamino K, Neumann T, Yang M, Wahlers A, Fehse B, Reuther GW, Schlegelberger B, Ganser A, Baum C, Li Z.
  Leukemia. 2007 Oct;21(10):2171-80. Epub 2007 Aug 2.

• Cell-culture assays reveal the importance of retroviral vector design for insertional genotoxicity.
  Modlich U, Bohne J, Schmidt M, von Kalle C, Knöss S, Schambach A, Baum C.
  Blood. 2006 Oct 15;108(8):2545-53. Epub 2006 Jul 6.

• HOXB4 inhibits cell growth in a dose-dependent manner and sensitizes cells towards extrinsic cues.
  Will E, Speidel D, Wang Z, Ghiaur G, Rimek A, Schiedlmeier B, Williams DA, Baum C, Ostertag W, Klump H.
  Cell Cycle. 2006 Jan;5(1):14-22. Epub 2006 Jan 9.

• Expression of the p75 neurotrophin receptor in acute leukaemia.
  Beutel G, Meyer J, Ma L, Yin S, Eder M, von Neuhoff N, Wilkens L, Wei J, Hertenstein B, Heil G, Schlegelberger B, Ganser A, Li Z, Baum C.
  Br J Haematol. 2005 Oct;131(1):67-70.

• HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells.
  Pilat S, Carotta S, Schiedlmeier B, Kamino K, Mairhofer A, Will E, Modlich U, Steinlein P, Ostertag W, Baum C, Beug H, Klump H.
  Proc Natl Acad Sci U S A. 2005 Aug 23;102(34):12101-6. Epub 2005 Aug 10.

• Control of self-renewal and differentiation of hematopoietic stem cells: HOXB4 on the threshold.
  Klump H, Schiedlmeier B, Baum C.
  Ann N Y Acad Sci. 2005 Jun;1044:6-15. Review.

• Leukemias following retroviral transfer of multidrug resistance 1 (MDR1) are driven by combinatorial insertional mutagenesis.
  Modlich U, Kustikova OS, Schmidt M, Rudolph C, Meyer J, Li Z, Kamino K, von Neuhoff N, Schlegelberger B, Kuehlcke K, Bunting KD, Schmidt S, Deichmann A, von Kalle C, Fehse B, Baum C.
  Blood. 2005 Jun 1;105(11):4235-46. Epub 2005 Feb 15.

• Tumor cells escape suicide gene therapy by genetic and epigenetic instability.
  Frank O, Rudolph C, Heberlein C, von Neuhoff N, Schröck E, Schambach A, Schlegelberger B, Fehse B, Ostertag W, Stocking C, Baum C.
  Blood. 2004 Dec 1;104(12):3543-9. Epub 2004 Aug 12.

• High-level ectopic HOXB4 expression confers a profound in vivo competitive growth advantage on human cord blood CD34+ cells, but impairs lymphomyeloid differentiation.
  Schiedlmeier B, Klump H, Will E, Arman-Kalcek G, Li Z, Wang Z, Rimek A, Friel J, Baum C, Ostertag W.
  Blood. 2003 Mar 1;101(5):1759-68. Epub 2002 Oct 24.

• Persisting multilineage transgene expression in the clonal progeny of a hematopoietic stem cell.
  Li Z, Fehse B, Schiedlmeier B, Düllmann J, Frank O, Zander AR, Ostertag W, Baum C.
  Leukemia. 2002 Sep;16(9):1655-63.

• Murine leukemia induced by retroviral gene marking.
  Li Z, Düllmann J, Schiedlmeier B, Schmidt M, von Kalle C, Meyer J, Forster M, Stocking C, Wahlers A, Frank O, Ostertag W, Kühlcke K, Eckert HG, Fehse B, Baum C.
  Science. 2002 Apr 19;296(5567):497. No abstract available.

• Human multidrug resistance-1 gene transfer to long-term repopulating human mobilized peripheral blood progenitor cells.
  Schiedlmeier B, Wermann K, Kühlcke K, Eckert HG, Baum C, Fruehauf S, Zeller WJ.
  Bone Marrow Transplant. 2000 May;25 Suppl 2:S118-24.

• Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice.
  Schiedlmeier B, Kühlcke K, Eckert HG, Baum C, Zeller WJ, Fruehauf S.
  Blood. 2000 Feb 15;95(4):1237-48.