Regenerative Medicine

Further publications:   Gene Therapy     Vectorology / Biosafety     Hematopoiesis

2022

• Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies
  Wunderlich S, Haase A, Merkert S, Jahn K, Deest M, Frieling H, Glage S, Korte W, Martens A, Kirschning A, Zeug A, Ponimaskin E, Göhring G, Ackermann M, Lachmann N, Moritz T, Zweigerdt R, Martin U. . Mol Ther Methods Clin Dev 2022;26:84-94
  DOI: 10.1016/j.omtm.2022.05.011
   
• Meteorin-like promotes heart repair through endothelial KIT receptor tyrosine kinase.
  Reboll MR, Klede S, Taft MH, Cai CL, Field LJ, Lavine KJ, Koenig AL, Fleischauer J, Meyer J, Schambach A, Niessen HW, Kosanke M, van den Heuvel J, Pich A, Bauersachs J, Wu X, Zheng L, Wang Y, Korf-Klingebiel M, Polten F, Wollert KC.
  Science 2022;376(6599):1343-1347
  DOI: 10.1126/science.abn3027
   

• Airway basal cells show a dedifferentiated KRT17 high Phenotype and promote fibrosis in idiopathic pulmonary fibrosis
  Jaeger B, Schupp JC, Plappert L, Terwolbeck O, Artysh N, Kayser G, Engelhard P, Adams TS, Zweigerdt R, Kempf H, Lienenklaus S, Garrels W, Nazarenko I, Jonigk D, Wygrecka M, Klatt D, Schambach A, Kaminski N, Prasse A.
  Nat Commun 2022;13(1):5637
  DOI: 10.1038/s41467-022-33193-0

2021

• Generation of hiPSC-derived low threshold mechanoreceptors containing axonal termini resembling bulbous sensory nerve endings and expressing Piezo1 and Piezo2.
  Zhu S, Stanslowsky N, Fernandez-Trillo J, Mamo TM, Yu P, Kalmbach N, Ritter B, Eggenschwiler R, Ouwendijk WJD, Mzinza D, Tan L, Leffler A, Spohn M, Brown RJP, Kropp KA, Kaever V, Ha TC, Narayanan P, Grundhoff A, Förster R, Schambach A, Verjans GMGM, Schmidt M, Kispert A, Cantz T, Gomis A, Wegner F, Viejo-Borbolla A.
  Stem Cell Res 2021;56:102535
  DOI: 10.1016/j.scr.2021.102535
   
• Therapeutic HNF4A mRNA attenuates liver fibrosis in a preclinical model.
  Yang T, Poenisch M, Khanal R, Hu Q, Dai Z, Li R, Song G, Yuan Q, Yao Q, Shen X, Taubert R, Engel B, Jaeckel E, Vogel A, Falk CS, Schambach A, Gerovska D, Arauzo-Bravo MJ, Vondran FWR, Cantz T, Horscroft N, Balakrishnan A, Chevessier F, Ott M, Sharma AD.
  J Hepatol 2021;75(6):1420-1433
  DOI: 10.1016/j.jhep.2021.08.011
   
• Conditionally immortalised leukaemia initiating cells co-expressing Hoxa9/Meis1 demonstrate microenvironmental adaptation properties ex vivo while maintaining myelomonocytic memory.
  Stahlhut M, Ha TC, Takmakova E, Morgan MA, Schwarzer A, Schaudien D, Eder M, Schambach A, Kustikova OS.
  Sci Rep 2021;11(1):5294
  DOI: 10.1038/s41598-021-84468-3
   
• Impaired immune response mediated by prostaglandin E2 promotes severe COVID-19 disease.
  Ricke-Hoch M, Stelling E, Lasswitz L, Gunesch AP, Kasten M, Zapatero-Belinchon FJ, Brogden G, Gerold G, Pietschmann T, Montiel V, Balligand JL, Facciotti F, Hirsch E, Gausepohl T, Elbahesh H, Rimmelzwaan GF, Hofer A, Kühnel MP, Jonigk D, Eigendorf J, Tegtbur U, Mink L, Scherr M, Illig T, Schambach A, Pfeffer TJ, Hilfiker A, Haverich A, Hilfiker-Kleiner D.
  PLoS One 2021;16(8):e0255335
  DOI: 10.1371/journal.pone.0255335

2020

• β-Catenin safeguards the ground state of mousepluripotency by strengthening the robustness of the transcriptional apparatus
  Meng Zhang, Yiwei Lai, Vladislav Krupalnik, Pengcheng Guo, Xiangpeng Guo, Jianguo Zhou, Yan Xu, Zhijun Yu, Longqi Liu, Ao Jiang, Wenjuan Li, Mazid Md Abdul, Gang Ma, Na Li, Xiuling Fu, Yuan Lv, Mengling Jiang, Muqddas Tariq, Shahzina Kanwal, Hao Liu, Xueting Xu, Hui Zhang, Yinghua Huang, Lulu Wang, Shuhan Chen, Isaac A Babarinde, Zhiwei Luo, Dongye Wang, Tiantian Zhou, Carl Ward, Minghui He, David P Ibañez, Yunpan Li, Jiajian Zhou, Jie Yuan, Yayan Feng, Karthik Arumugam, Umberto Di Vicino, Xichen Bao, Guangming Wu, Axel Schambach, Huating Wang, Hao Sun, Fei Gao, Baoming Qin, Andrew P Hutchins, Bradley W Doble, Christine Hartmann, Maria Pia Cosma, Yan Qin, Guo-Liang Xu, Runsheng Chen, Giacomo Volpe, Liang Chen, Jacob H Hanna, Miguel A  Esteban
  Sci Adv. 2020 Jul 17;6(29):eaba1593.doi: 10.1126/sciadv.aba1593. 
   

• Hematopoietic stem-cell senescence and myocardial repair - Coronary artery disease genotype/phenotype analysis of post-MI myocardial regeneration response induced by CABG/CD133+ bone marrow hematopoietic stem cell treatment in RCT PERFECT Phase 3
  Markus Wolfien, Denise Klatt, Amankeldi A. Salybekov, Masaaki Ii, Miki Komatsu-Horii, Ralf Gaebel, Julia Philippou-Massier, Eric Schrinner, Hiroshi Akimaru, Erika Akimarue
  Robert David, Jens Garbade, Jan Gummert, Axel Haverich, Holger Hennig, Hiroto Iwasaki, Alexander Kaminski, Atsuhiko Kawamoto, Christian Klopsch, Johannes T. Kowallick,
  Stefan Krebs, Julia Nesteruk, Hermann Reichenspurner, Christian Ritter, Christof Stamm, Ayumi Tani-Yokoyama, Helmut Blum, Olaf Wolkenhauer, Axel Schambach,
  Takayuki Asahara, Gustav Steinhoff
  EBioMedicine 57 (2020) https://doi.org/10.1016/j.ebiom.2020.102862
   

• Generation of an induced pluripotent stem cell line (MHHi018-A) from a patient with Cystic Fibrosis carrying p.Asn1303Lys (N1303K) mutation
  Sylvia Merkert, Madline Schubert, Alexandra Haase, Hettie M Janssens, Bob Scholte, Nico Lachmann, Gudrun Göhring, Ulrich Martin
  Stem Cell Res. 2020 Apr;44:101744.doi: 10.1016/j.scr.2020.101744. Epub 2020 Mar 25.
   

• Targeted Integration of Inducible Caspase-9 in Human iPSCs Allows Efficient in vitro Clearance of iPSCs and iPSC-Macrophages
  Alexandra Lipus, Ewa Janosz, Mania Ackermann, Miriam Hetzel, Julia Dahlke, Theresa Buchegger, Stephanie Wunderlich, Ulrich Martin, Toni Cathomen,
  Axel Schambach, Thomas Moritz and Nico Lachmann
  Int. J. Mol. Sci. 2020, 21, 2481; doi:10.3390/ijms21072481 
   

• Gap Junction Dependent Cell Communication Is Modulated During Transdifferentiation of Mesenchymal Stem/Stromal Cells Towards Neuron-Like Cells
  Nadine Dilger, Anna-Lena Neehus, Klaudia Grieger, Andrea Hoffmann, Max Menssen and Anaclet Ngezahayo
  Front. Cell Dev. Biol., 31 August 2020 Sec. Stem Cell Research Volume 8 - 2020 | https://doi.org/10.3389/fcell.2020.00869
   

• Human STAT1 gain-of-function iPSC line from a patient suffering from chronic mucocutaneous candidiasis
  Kathrin Haake, Tim Wüstefeld, Sylvia Merkert, Doreen Lüttge, Gudrun Göhring, Bernd Auber, Ulrich Baumann, Nico Lachmann
  Stem Cell Res. 2020 Mar;43:101713.doi: 10.1016/j.scr.2020.101713. Epub 2020 Jan 17.

2019

• Concise Review: Towards the Clinical Translation of Induced Pluripotent Stem Cell-Derived Blood Cells — Ready for Take-Off
  Kathrin Haake, Mania, Ackermann, Nico Lachmann
  Stem Cells Transl Med. 2019 Apr;8(4):332-339.doi: 10.1002/sctm.18-0134. Epub 2018 Dec 26.
   
• New Drugs for an Old Foe: Mycobacterium tuberculosis Meets PSC-Derived Macrophages
  Kathrin Haake, Nico Lachmann
  Stem Cell Reports |Vol. 13 |957–959 |December 10, 2019

2018

• A patient-specific induced pluripotent stem cell model for West syndrome caused by ST3GAL3 deficiency.
  van Diepen L, Buettner FFR, Hoffmann D, Thiesler CT, von Bohlen Und Halbach O, von Bohlen Und Halbach V, Jensen LR, Steinemann D, Edvardson S, Elpeleg O, Schambach A, Gerardy-Schahn R, Kuss AW.
  Eur J Hum Genet. 2018 Dec;26(12):1773-1783. doi: 10.1038/s41431-018-0220-5.
   
• An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages.
  Beneke V, Küster F, Neehus AL, Hesse C, Lopez-Rodriguez E, Haake K, Rafiei Hashtchin A, Schott JW, Walter D, Braun A, Wolkers WF, Ackermann M, Lachmann N.
  Sci Rep. 2018 Nov 2;8(1):16281. doi: 10.1038/s41598-018-34524-2.
   
• Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections.
  Ackermann M, Kempf H, Hetzel M, Hesse C, Hashtchin AR, Brinkert K, Schott JW, Haake K, Kühnel MP, Glage S, Figueiredo C, Jonigk D, Sewald K, Schambach A, Wronski S, Moritz T, Martin U, Zweigerdt R, Munder A, Lachmann N.
  Nat Commun. 2018 Nov 30;9(1):5088. doi: 10.1038/s41467-018-07570-7.
   
• Concise Review: Towards the Clinical Translation of Induced Pluripotent Stem Cell-Derived Blood Cells-Ready for Take-Off.
  Haake K, Ackermann M, Lachmann N.
  Stem Cells Transl Med. 2019 Apr;8(4):332-339. doi: 10.1002/sctm.18-0134.
   
• Expansion of functional personalized cells with specific transgene combinations.
  Lipps C, Klein F, Wahlicht T, Seiffert V, Butueva M, Zauers J, Truschel T, Luckner M, Köster M, MacLeod R, Pezoldt J, Hühn J, Yuan Q, Müller PP, Kempf H, Zweigerdt R, Dittrich-Breiholz O, Pufe T, Beckmann R, Drescher W, Riancho J, Sañudo C, Korff T, Opalka B, Rebmann V, Göthert JR, Alves PM, Ott M, Schucht R, Hauser H, Wirth D, May T.
  Nat Commun. 2018 Mar 8;9(1):994. doi: 10.1038/s41467-018-03408-4.
   
• Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines.
  Becker J, Kinast V, Döring M, Lipps C, Duran V, Spanier J, Tegtmeyer PK, Wirth D, Cicin-Sain L, Alcamí A, Kalinke U.
  Virulence. 2018;9(1):1669-1684. doi: 10.1080/21505594.2018.1535785.
   
• Human Teratoma-Derived Hematopoiesis Is a Highly Polyclonal Process Supported by Human Umbilical Vein Endothelial Cells.
  Philipp F, Selich A, Rothe M, Hoffmann D, Rittinghausen S, Morgan MA, Klatt D, Glage S, Lienenklaus S, Neuhaus V, Sewald K, Braun A, Schambach A.
  Stem Cell Reports. 2018 Nov 13;11(5):1051-1060. doi: 10.1016/j.stemcr.2018.09.010.
   
• iPSC-Derived Macrophages Effectively Treat Pulmonary Alveolar Proteinosis in Csf2rb-Deficient Mice.
  Mucci A, Lopez-Rodriguez E, Hetzel M, Liu S, Suzuki T, Happle C, Ackermann M, Kempf H, Hillje R, Kunkiel J, Janosz E, Brennig S, Glage S, Bankstahl JP, Dettmer S, Rodt T, Gohring G, Trapnell B, Hansen G, Trapnell C, Knudsen L, Lachmann N, Moritz T.
  Stem Cell Reports. 2018 Sep 11;11(3):696-710. doi: 10.1016/j.stemcr.2018.07.006.
   
• Murine alveolar epithelial cells and their lentivirus-mediated immortalisation.
  Sapich S, Hittinger M, Hendrix-Jastrzebski R, Repnik U, Griffiths G, May T, Wirth D, Bals R, Schneider-Daum N, Lehr CM.
  Altern Lab Anim. 2018 May;46(2):73-89.

2017

• Ex vivo Generation of Genetically Modified Macrophages from Human Induced Pluripotent Stem Cells.
  Ackermann M, Kuhn A, Kunkiel J, Merkert S, Martin U, Moritz T, Lachmann N
  Transfus Med Hemother. 2017 Jun;44(3):135-142. doi: 10.1159/000477129.

• Rapid and efficient generation of oligodendrocytes from human induced pluripotent stem cells using transcription factors.
  Ehrlich M, Mozafari S, Glatza M, Starost L, Velychko S, Hallmann AL, Cui QL, Schambach A, Kim KP, Bachelin C, Marteyn A, Hargus G, Johnson RM, Antel J, Sterneckert J, Zaehres H, Scholer HR, Baron-Van Evercooren A, Kuhlmann T.
  Proc Natl Acad Sci U S A 2017;114(11):E2243-E2252

• Potent and reversible lentiviral vector restriction in murine induced pluripotent stem cells.
  Geis FK, Galla M, Hoffmann D, Kuehle J, Zychlinski D, Maetzig T, Schott JW, Schwarzer A, Goffinet C, Goff SP, Schambach A.
  Retrovirology 2017;14(1):34

• Detailed comparison of retroviral vectors and promoter configurations for stable and high transgene expression in human induced pluripotent stem cells.
  Hoffmann D, Schott JW, Geis FK, Lange L, Müller FJ, Lenz D, Zychlinski D, Steinemann D, Morgan M, Moritz T, Schambach A.
  Gene Ther 2017;24(5):298-307

• TALEN-mediated functional correction of human iPSC-derived macrophages in context of hereditary pulmonary alveolar proteinosis.
  Kuhn A, Ackermann M, Mussolino C, Cathomen T, Lachmann N, Moritz T.
  Sci Rep 2017;7(1):15195

• Forced expression of human macrophage colony-stimulating factor in CD34(+) cells promotes monocyte differentiation in vitro and in vivo but blunts osteoclastogenesis in vitro.
  Montano Almendras CP, Thudium CS, Löfvall H, Moscatelli I, Schambach A, Henriksen K, Richter J.
  Eur J Haematol 2017;98(5):517-526

• Impaired IFNgamma-Signaling and Mycobacterial Clearance in IFNgammaR1-Deficient Human iPSC-Derived Macrophages.
  Neehus AL, Lam J, Haake K, Merkert S, Schmidt N, Mucci A, Ackermann M, Schubert M, Happle C, Kühnel MP, Blank P, Philipp F, Goethe R, Jonigk D, Martin U, Kalinke U, Baumann U, Schambach A, Roesler J, Lachmann N.
  Stem Cell Reports 2017:DOI:10.1016/j.stemcr.2017.11.011

• Inhibition of miRNA-212/132 improves the reprogramming of fibroblasts into induced pluripotent stem cells by de-repressing important epigenetic remodelling factors.
  Pfaff N, Liebhaber S, Möbus S, Beh-Pajooh A, Fiedler J, Pfanne A, Schambach A, Thum T, Cantz T, Moritz T.
  Stem Cell Res 2017;20:70-75

• Gene correction of HAX1 reversed Kostmann disease phenotype in patient-specific induced pluripotent stem cells.
  Pittermann E, Lachmann N, MacLean G, Emmrich S, Ackermann M, Göhring G, Schlegelberger B, Welte K, Schambach A, Heckl D, Orkin SH, Cantz T, Klusmann JH.
  Blood Adv 2017;1(14):903-914

2016

• Massive Clonal Selection and Transiently Contributing Clones During Expansion of Mesenchymal Stem Cell Cultures Revealed by Lentiviral RGB-Barcode Technology.
  Selich A, Daudert J, Hass R, Philipp F, von Kaisenberg C, Paul G, Cornils K, Fehse B, Rittinghausen S, Schambach A, Rothe M.
  Stem Cells Transl Med. 2016 May;5(5):591-601. doi: 10.5966/sctm.2015-0176.

• Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.
  Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel MP, Ackermann M, Happle C, Kuhn A, Schambach A, Trapnell BC, Hansen G, Moritz T, Lachmann N.
  Stem Cell Reports. 2016 Aug 9;7(2):292-305. doi: 10.1016/j.stemcr.2016.06.011.

• Glycomic Characterization of Induced Pluripotent Stem Cells Derived from a Patient Suffering from Phosphomannomutase 2 Congenital Disorder of Glycosylation (PMM2-CDG).
  Thiesler CT, Cajic S, Hoffmann D, Thiel C, van Diepen L, Hennig R, Sgodda M, Weiβmann R, Reichl U, Steinemann D, Diekmann U, Huber NM, Oberbeck A, Cantz T, Kuss AW, Körner C, Schambach A, Rapp E, Buettner FF.
  Mol Cell Proteomics. 2016 Apr;15(4):1435-52. doi: 10.1074/mcp.M115.054122.

• MicroRNA-Based Therapy of GATA2-Deficient Vascular Disease.
  Hartmann D, Fiedler J, Sonnenschein K, Just A, Pfanne A, Zimmer K, Remke J, Foinquinos A, Butzlaff M, Schimmel K, Maegdefessel L, Hilfiker-Kleiner D, Lachmann N, Schober A, Froese N, Heineke J, Bauersachs J, Batkai S, Thum T.
  Circulation. 2016 Dec 13;134(24):1973-1990.

• Biphasic modulation of Wnt signaling supports efficient foregut endoderm formation from human pluripotent stem cells.
  Hoepfner J, Kleinsorge M, Papp O, Ackermann M, Alfken S, Rinas U, Solodenko W, Kirschning A, Sgodda M, Cantz T.
  Cell Biol Int. 2016 May;40(5):534-48. doi: 10.1002/cbin.10590.

• Lung surfactant metabolism: early in life, early in disease and target in cell therapy.
  Lopez-Rodriguez E, Gay-Jordi G, Mucci A, Lachmann N, Serrano-Mollar A.
  Cell Tissue Res. 2016 Oct 25. [Epub ahead of print] Review.

• Long noncoding RNA Chast promotes cardiac remodeling.
  Viereck J, Kumarswamy R, Foinquinos A, Xiao K, Avramopoulos P, Kunz M, Dittrich M, Maetzig T, Zimmer K, Remke J, Just A, Fendrich J, Scherf K, Bolesani E, Schambach A, Weidemann F, Zweigerdt R, de Windt LJ, Engelhardt S, Dandekar T, Batkai S, Thum T.
  Sci Transl Med. 2016 Feb 17;8(326):326ra22. doi: 10.1126/scitranslmed.aaf1475.

• Direct Reprogramming of Hepatic Myofibroblasts into Hepatocytes In Vivo Attenuates Liver Fibrosis.
  Song G, Pacher M, Balakrishnan A, Yuan Q, Tsay HC, Yang D, Reetz J, Brandes S, Dai Z, Pützer BM, Araúzo-Bravo MJ, Steinemann D, Luedde T, Schwabe RF, Manns MP, Schöler HR, Schambach A, Cantz T, Ott M, Sharma AD.
  Cell Stem Cell. 2016 Jun 2;18(6):797-808. doi: 10.1016/j.stem.2016.01.010.

• Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase.
  Eggenschwiler R, Moslem M, Fráguas MS, Galla M, Papp O, Naujock M, Fonfara I, Gensch I, Wähner A, Beh-Pajooh A, Mussolino C, Tauscher M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, Cathomen T, Cantz T.
  Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.

• Prevention of allograft rejection by use of regulatory T cells with a MHC-specific chimeric antigen receptor.
  Noyan F, Zimmermann K, Hardtke-Wolenski M, Knoefel A, Schulde E, Geffers R, Hust M, Huehn J, Galla M, Morgan M, Jokuszies A, Manns MP, Jaeckel E.
  Am J Transplant. 2016 Dec 20. doi: 10.1111/ajt.14175. [Epub ahead of print]

• Generation of HLA-universal iPSCs-derived megakaryocytes and platelets for survival under refractoriness conditions.
  Börger AK, Eicke D, Wolf C, Gras C, Aufderbeck S, Schulze K, Engels L, Eiz-Vesper B, Schambach A, Guzman CA, Lachmann N, Moritz T, Martin U, Blasczyk R, Figueiredo C.
  Mol Med. 2016 May 16;22. doi: 10.2119/molmed.2015.00235. [Epub ahead of print]

2015

• Production of mature healthy hematopoietic cells from induced pluripotent stem cells derived from an AML diagnostic sample containing the t(8;21) translocation.
  Hoffmann D, Göhring G, Heuser M, Ganser A, Schambach A, Morgan MA.
  Stem Cells. 2015 Sep 21. doi: 10.1002/stem.2207. [Epub ahead of print]

• Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies.
  Lachmann N, Ackermann M, Frenzel E, Liebhaber S, Brennig S, Happle C, Hoffmann D, Klimenkova O, Lüttge D, Buchegger T, Kühnel MP, Schambach A, Janciauskiene S, Figueiredo C, Hansen G, Skokowa J, Moritz T.
  Stem Cell Reports. 2015 Feb 10;4(2):282-96. doi: 10.1016/j.stemcr.2015.01.005.

2014

• FAS-based cell depletion facilitates the selective isolation of mouse induced pluripotent stem cells.
  Warlich E, Schambach A, Lock D, Wedekind D, Glage S, Eckardt D, Bosio A, Knöbel S.
  PLoS One. 2014 Jul 16;9(7):e102171. doi: 10.1371/journal.pone.0102171. eCollection 2014.

• Differential role of nonhomologous end joining factors in the generation, DNA damage response, and myeloid differentiation of human induced pluripotent stem cells.
  Felgentreff K, Du L, Weinacht KG, Dobbs K, Bartish M, Giliani S, Schlaeger T, DeVine A, Schambach A, Woodbine LJ, Davies G, Baxi SN, van der Burg M, Bleesing J, Gennery A, Manis J, Pan-Hammarström Q, Notarangelo LD.
  Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8889-94. doi: 10.1073/pnas.1323649111. Epub 2014 Jun 2.

• Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based gene therapy.
  Ackermann M, Lachmann N, Hartung S, Eggenschwiler R, Pfaff N, Happle C, Mucci A, Göhring G, Niemann H, Hansen G, Schambach A, Cantz T, Zweigerdt R, Moritz T.
  Biomaterials. 2014 Feb;35(5):1531-42. doi: 10.1016/j.biomaterials.2013.11.024. Epub 2013 Nov 26.

2013

• Toward position-independent retroviral vector expression in pluripotent stem cells.
  Schambach A, Moritz T.
  Mol Ther. 2013 Aug;21(8):1474-7. doi: 10.1038/mt.2013.161. No abstract available.

• A ubiquitous chromatin opening element prevents transgene silencing in pluripotent stem cells and their differentiated progeny.
  Pfaff N, Lachmann N, Ackermann M, Kohlscheen S, Brendel C, Maetzig T, Niemann H, Antoniou MN, Grez M, Schambach A, Cantz T, Moritz T.
  Stem Cells. 2013 Mar;31(3):488-99. doi: 10.1002/stem.1316.

2012

• Efficient hematopoietic redifferentiation of induced pluripotent stem cells derived from primitive murine bone marrow cells.
  Pfaff N, Lachmann N, Kohlscheen S, Sgodda M, Araúzo-Bravo MJ, Greber B, Kues W, Glage S, Baum C, Niemann H, Schambach A, Cantz T, Moritz T.
  Stem Cells Dev. 2012 Mar 20;21(5):689-701. doi: 10.1089/scd.2011.0010. Epub 2011 Aug 24.

2011

• miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2.
  Pfaff N, Fiedler J, Holzmann A, Schambach A, Moritz T, Cantz T, Thum T.
  EMBO Rep. 2011 Oct 28;12(11):1153-9. doi: 10.1038/embor.2011.176.

• Optimal reprogramming factor stoichiometry increases colony numbers and affects molecular characteristics of murine induced pluripotent stem cells.
  Tiemann U, Sgodda M, Warlich E, Ballmaier M, Schöler HR, Schambach A, Cantz T.
  Cytometry A. 2011 Jun;79(6):426-35. doi: 10.1002/cyto.a.21072. Epub 2011 May 4.

• Lentiviral vector design and imaging approaches to visualize the early stages of cellular reprogramming.
  Warlich E, Kuehle J, Cantz T, Brugman MH, Maetzig T, Galla M, Filipczyk AA, Halle S, Klump H, Schöler HR, Baum C, Schroeder T, Schambach A.
  Mol Ther. 2011 Apr;19(4):782-9. doi: 10.1038/mt.2010.314. Epub 2011 Feb 1.

2007 - 2010

• Generation and genetic modification of induced pluripotent stem cells.
  Schambach A, Cantz T, Baum C, Cathomen T.
  Expert Opin Biol Ther. 2010 Jul;10(7):1089-103. doi: 10.1517/14712598.2010.496775. Review.

• Zinc-finger nucleases meet iPS cells: Zinc positive: tailored genome engineering meets reprogramming.
  Cathomen T, Schambach A.
  Gene Ther. 2010 Jan;17(1):1-3. doi: 10.1038/gt.2009.151. Epub 2009 Nov 12. No abstract available.

• HOXB4's road map to stem cell expansion.
  Schiedlmeier B, Santos AC, Ribeiro A, Moncaut N, Lesinski D, Auer H, Kornacker K, Ostertag W, Baum C, Mallo M, Klump H.
  Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16952-7. Epub 2007 Oct 16.