Hannover Medical School
Principal Investigator: Dr. Robert Zweigerdt, TECHNOBEAT Coordinator
Principal Investigator: Prof. Ulrich Martin, LEBAO Director
Co-principal investigator: Dr. Ruth Olmer
Areas of expertise
- Induction of hiPSCs by somatic cell reprogramming
- hiPSCs targeting by homologous recombination using up-to-date technologies such as TALENs and CRISPR/Cas9
- hiPSC suspension culture and mass expansion in controlled bioreactors including cardiomyogenic differentiation
- Induction, characterization and mass production of cardiac micro tissues
- hiPSC suspension culture and endothelial differentiation
Main tasks:
Work Package 1
- Derive transgene-free hiPSC lines via SOP-based protocols
- hiPSC line characterization
- Reporter line engineering for in situ tracing
- hiPSC line cryopreservation, banking and distribution to network members
Work Package 5
- Cardiac differentiation in chemically defined medium
- Scale up hiPSC-CM production to 500 - 1,000 ml bioreactor scale
- Develop SOPs for hiPSC-CM
- µ-tissue generation and storage
- hiPSC-CMs / µ-tissues in vitro characterization including arrhythmogenic and electromechanical properties
- µ-tissues in vivo characterization; safety and function in rodents
Work Package 10
- Project coordination and management
- Project controlling and contractual management
- Resources management
- Communication and scientific exchange management
- Dissemination
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