Basic practical course in biochemistry
The basic practical course in biochemistry covers the practical basics of biochemistry.
Head of the practical course: Prof. Dr. Dr. Halyna Shcherbata
Organization and supervision: Kathrin Laaß
Technical supervision: Stefanie Reuß
Location: MHH, Building I2, Theoretical Teaching Building I.
The basic practical course in biochemistry takes place over 10 days. There will be 10 experiments (1 experiment / day).
Start at 9.15 a.m. with a preliminary discussion of the experiment. Each student then carries out the experiment independently in the laboratory.
After a lunch break, the protocol (introduction, execution, discussion of the results) is drawn up.
This is followed by a debriefing on the experiment. End of the practical day approx. 16.00 hrs.
Dates and registration for the next courses
Information on the current courses and registration is available via the Stud.IP system of Leibniz Universität Hannover.
Students of other study programs (Chemistry, FüBa) can participate in the courses by arrangement (laass.kathrin(at)mh-hannover.de).
Contents
1st experiment:
a) Isolation of DNA from eukaryotic cells and b) Plasmid analysis/restriction digestion.
2nd experiment:
a) Transamination of alanine to glutamate and thin-layer chromatography and b) Deamination of asparagine and determination of the concentration of ammonia and aspartic acid.
3rd experiment:
a) glycolysis, inhibition of ethanol formation from glucose and b) acidic and enzymatic hydrolysis of starch.
4th experiment:
a) Determination of the Michaelis constants of lactate dehydrogensase b) Representation of the Michalis-Menten graph and the Lineweaver-Burk graph.
5th experiment:
Protein cleavage of casein by a) trypsin and b) chymotrypsin. Protein detection methods: Biuret and Lowry
6th experiment:
Determination of the specificity of coenzymes and amino acids of glutamate dehydrogenase.
7th experiment:
Detection of the specific activity of alkaline and acid phosphatase in retinoic acid-induced mammalian cells.
8. experiment:
a) Determination of the pH optimum of ß-fructosidase and b) Enzymatic cleavage of sucrose
9th experiment:
Analysis of membrane lipids a) Determination of phosphatidylcholine and phosphatidyl ethanolamine via phosphate determination and b) thin layer chromatography.
10th experiment:
Method of SDS gel electrophoresis, protein separation and identification of unknown protein samples using gel electrophoresis.