Tim Holler
Medical technologist for laboratory analysis (MTL)
Contact
Tel: +49 511 532 -2757 (office), -3654 (laboratory)
Fax: +49 511 532-161215
Address: 30625 Hanover, Carl-Neuberg-Str.1
Office: Building J03, Block 01, Level 03, Room 1320
Career
- Since October 2020
Studied Human Medicine at Hannover Medical School (MHH)
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Since October 2016
Medical Technologist for Laboratory Analysis (MTL, formerly: "MTLA") (MTLA) at the Institute of Molecular and Cell Physiology at Hannover Medical School (MHH) -
2015 to 2017
Study and team assistant at the Hannover Clinical Trial Center GmbH -
2013 to 2016
School-based training as a medical-technical laboratory assistant (MTLA) at Hannover Medical School -
2012 to 2013
Voluntary Scientific Year (FWJ) at the Institute of Molecular and Cell Physiology at Hannover Medical School (MHH); research focus: investigation of familial hypertrophic cardiomyopathy at the molecular level using the "In Vitro Motility Assay" -
2012
Abitur at the Elsa-Brändström-Schule Hannover (grammar school) with a focus on natural sciences (chemistry, biology, mathematics)
Current research focus
- Differential expression ratio of mutant to wild-type allele in individual cardiomyocytes as a new concept for the pathogenesis of familial hypertrophic cardiomyopathy
- Maturation of human pluripotent stem cell cardiomyocytes to an adult ventricular phenotype as a cell culture model for disease modeling, cell replacement therapy, drug screening, etc.
- Effect of cardiac myosin isoforms on functional properties of vital cardiomyocytes
Expertise
- Immunohistochemistry, immunofluorescence staining, imaging, morphological and protein biochemical analysis of primary cardiomyocytes and cell culture models at the single cell level, single cell mapping, preparation of histological slides, cell culture
- Graphic and Power Point layout, "Art of Science"
- Supervision of FWJ students and PhD students, assistance with student teaching
2025
- Differential impact of substrates on myosin heavy and light chain expression in human stem cell-derived cardiomyocytes at single-cell level, Felix Osten, Alea K Bodenschatz, Karina Ivaskevica, Simon Kröhn, Birgit Piep, Tim Holler, Jana Teske, Judith Montag, Bogdan Iorga, Natalie Weber, Robert Zweigerdt, Theresia Kraft, Joachim D Meissner, J Muscle Res Cell Motil. 2025 Feb 13. doi: 10.1007/s10974-025-09690-2. online ahead of print. PubMed
- Patient-specific hiPSC-derived cardiomyocytes indicate allelic and contractile imbalance as pathogenic factor in early-stage hypertrophic cardiomyopathy, Natalie Weber, Judith Montag, Kathrin Kowalsk, Bogdan Iorga, Jeanne de la Roche, Tim Holler, Daniel Wojciechowski, Meike Wendland, Ante Radocaj, Anne-Kathrin Mayer, Anja Brunkhorst, Felix Osten, Valentin Burkart, Birgit Piep, Alea Bodenschatz, Pia Gibron, Kristin Schwanke, Annika Franke, Stefan Thiemann, Anastasia Koroleva, Angelika Pfanne, Maike Konsanke, Jan Fiedler, Jan Hegermann, Christoph Wredej, Christian Mühlfeld, Boris Chichkov, Martin Fischer, Thomas Thum, Antonio Francino, Ulrich Martin, Joachim Meißner, Robert Zweigerdt, Theresia Kraft, Journal of Molecular and Cellular Cardiology (JMCC) Volume 198, January 2025, Pages 112-125, https://doi.org/10.1016/j.yjmcc.2024.11.007; PubMed
2023
- Myosin expression and contractile function are altered by replating stem cell-derived cardiomyocytes, Osten, F., Weber, N., Wendland, M., Holler, T., Piep, B., Kröhn, S., Teske, J., Bodenschatz, A.K., Devadas, S.B., Menge, K.S., Chatterjee, S., Schwanke, K., Kosanke, M., Montag, J., Thum, T., Zweigerdt, R., Kraft, T., Iorga, B., and Meissner, J.D. (2023), J Gen Physiol (2023) 155 (11): e202313377, https://doi.org/10.1085/jgp.202313377, Online ahead of print
2022
- SENP7 deSUMOylase-governed transcriptional program coordinates sarcomere assembly and is targeted in muscle atrophy
Amrute-Nayak M, Gand LV, Khan B, Holler T, Kefalakes E, Kosanke M, Kraft T, Nayak A.
Cell Rep. 2022 Nov 22;41(8):111702. doi: 10.1016/j.celrep.2022.111702. PMID: 36417853. https://doi.org/10.1016/j.celrep.2022.111702
- Transcriptional bursts and heterogeneity among cardiomyocytes in hypertrophic cardiomyopathy,
Valentin Burkart, Kathrin Kowalski, David Aldag-Niebling, Julia Beck, Dirk Alexander Frick, Tim Holler, Ante Radocaj, Birgit Piep, Andre Zeug, Denise Hilfiker-Kleiner, Cristobal G. dos Remedios, Jolanda van der Velden, Judith Montag and Theresia Kraft,
Front. Cardiovasc. Med, August 2022, Sec. Cardiovascular Biologics and Regenerative Medicin, doi: 10.3389/fcvm.2022.987889
- Skeletal muscle derived Musclin protects the heart during pathological overload
Szaroszyk M, Kattih B, Martin-Garrido A, Trogisch FA, Dittrich GM, Grund A, Abouissa A, Derlin K, Meier M, Holler T, Korf-Klingebiel M, Völker K, Garfias Macedo T, Pablo Tortola C, Boschmann M, Huang N, Froese N, Zwadlo C, Malek Mohammadi M, Luo X, Wagner M, Cordero J, Geffers R, Batkai S, Thum T, Bork N, Nikolaev VO, Müller OJ, Katus HA, El-Armouche A, Kraft T, Springer J, Dobreva G, Wollert KC, Fielitz J, von Haehling S, Kuhn M, Bauersachs J, Heineke
J. Nat Commun. 2022 Jan 10;13(1):149. doi: 10.1038/s41467-021-27634-5. PMID: 35013221; PMCID: PMC8748430. https://www.nature.com/articles/s41467-021-27634-5
2021
- Chemotherapy triggers cachexia by deregulating synergetic function of histone-modifying enzymes
Amrute-Nayak M, Pegoli G, Holler T, Lopez-Davila AJ, Lanzuolo C, Nayak A.
J Cachexia Sarcopenia Muscle. 2021 Feb;12(1):159-176. doi: 10.1002/jcsm.12645. Epub 2020 Dec 10. PMID: 33305533; PMCID: PMC7890149.https://onlinelibrary.wiley.com/doi/full/10.1002/jcsm.12645
2020
- Advanced Single-Cell Mapping Reveals that in hESC Cardiomyocytes Contraction Kinetics and Action Potential Are Independent of Myosin Isoform,
Natalie Weber, Kathrin Kowalski, Tim Holler, Ante Radocaj, Martin Fischer, Stefan Thiemann, Jeanne de la Roche, Kristin Schwanke, Birgit Piep, Neele Peschel, Uwe Krumm, Alexander Lingk, Meike Wendland, Stephan Greten, Jan Dieter Schmitto, Issam Ismail, Gregor Warnecke, Urs Zywietz, Boris Chichkov, Joachim Meißner, Axel Haverich, Ulrich Martin, Bernhard Brenner, Robert Zweigerdt, Theresia Kraft,
stem cell report, Published:April 16, 2020, DOI: https://doi.org/10.1016/j.stemcr.2020.03.015, Journal free
2019
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Regulation of SETD7 Methyltransferase by SENP3 is Crucial for Sarcomere Organization and Cachexia
Arnab Nayak¹, Alfredo J. Lopez-Davila¹, Ekaterini Kefalakes², Tim Holler¹, Theresia Kraft¹, Mamta Amrute-Nayak¹
J. Cell Reports; Volume 27, Issue 9, 28 May 2019, Pages 2725-2736.e4; ¹Institute of Molecular and Cell Physiology; ²Department of Neurology, Hannover; Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany; doi: https://doi.org/10.1016/j.celrep.2019.04.107
2019
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Pluripotent stem cell-derived cardiomyocytes from a patient with Arg723Gly β-myosin heavy chain mutation as model for Hypertrophic Cardiomyopathy?
Natalie Weber, Tim Holler, Neele Peschel, Kristin Schwanke, Birgit Piep, Ulrich Martin, Robert Zweigerdt, Theresia Kraft
48th European Muscle Conference (EMC-Meeting Canterbury) -
In vitro heartbeat - The physiological characterization of heart muscle cells
Tim Holler; School Lectures of the Young Physiologists; 98th Meeting of the German Physiological Society (DPG Meeting Ulm) -
Twitch kinetics and action potential parameters are independent of expressed myosin heavy chain isoform in human embryonic stem cell-derived cardiomyocytes
Natalie Weber*, Kathrin Kowalski*, Tim Holler, Ante Radocaj, Martin Fischer, Jeanne de la Roche, Stefan Thiemann, Kristin Schwanke, Birgit Piep, Alexander Lingk, Uwe Krumm, Ulrich Martin, Robert Zweigerdt, Bernhard Brenner, Theresia Kraft; *authors contributed equally;
98th Meeting of the German Physiological Society (DPG Meeting Ulm) -
In human embryonic stem cell-derived cardiomyocytes twitch kinetics and action potential parameters are independent of the expressed myosin heavy chain isoform
Natalie Weber*, Kathrin Kowalski*, Tim Holler, Ante Radocaj, Martin Fischer, Jeanne de la Roche, Stefan Thiemann, Kristin Schwanke, Alexander Lingk, Uwe Krumm, Birgit Piep, Ulrich Martin, Robert Zweigerdt, Bernhard Brenner, Theresia Kraft; *authors contributed equally;
63rd Annual Meeting of the Biophysical Society (Biophysics Meeting Baltimore)
2018
- Human embryonic stem cell-derived cardiomyocytes: Single cell mapping to relate twitch kinetics to myosin heavy chain protein and mRNA expression
N. Weber*, K. Kowalski*, T. Holler, K. Schwanke, A. Radocaj, A. Lingk, U. Krumm, M. Wendland, U. Zywietz, B. Chichkov, U. Martin, R. Zweigerdt, B. Brenner, T. Kraft; *authors contributed equally; 62nd Annual Meeting of the Biophysical Society (Biophysics Meeting San Francisco); Biophysical Journal, Volume 114, Issue 3, Supplement 1, 2 February 2018, Page 549a;
https://doi.org/10.1016/j.bpj.2017.11.2999
2017
- Single cell mapping used to assign mRNA and protein expression of cardiac myosin heavy chain to twitch kinetics of the same human embryonic stem cell derived cardiomyocyte
N. Weber*, K. Kowalski*, T. Holler, A. Lingk, A. Radocaj, U. Krumm, M. Wendland, U. Zywietz, B. Chichkov, K. Schwanke, U. Martin, R. Zweigerdt, B. Brenner, T. Kraft; *authors contributed equally; 46th European Muscle Conference (EMC-Meeting Potsdam); 2715-Plat; 21 February 2018;
https://www.cell.com/biophysj/pdf/S0006-3495(17)34231-5.pdf
- Single cell mapping allows correlation between twitch contractions, mRNA and protein expression of cardiac myosin heavy chain of the same hES-CM
Natalie Weber* , Kathrin Kowalski*, Tim Holler, Ante Radocaj, Meike Wendland, Urs Zywietz, Boris Chichkov, Ulrich Martin, Kristin Schwanke, Robert Zweigerdt, Bernhard Brenner, Theresia Kraft; *authors contributed equally;
5th German Stem Cell Network (Annual GSCN Conference Jena); Abstract No. P066; page 156
2013
- The β-myosin mutation G741R in Familial Hypertrophic Cardiomyopathy
Montag, J., Weber, A.-L., Scholz, T, Hanke, E., Nemann, E., Matinmehr, F., Becker, E., T. Holler, Francino, A., Navarro-Lopez, F., Brenner, B., Kraft, T.; 92nd Meeting of the German Physiological Society
(DPG Meeting Heidelberg; Acta Physiologica 2013, Volume 207, Supplement 694)
