Becton-Dickinson FACSAria III Fusion ("Fusion")

4 air-cooled solid state lasers: 488 nm, 561 nm, 641 nm, 405 nm;
17 parameters, 15 colors

download complete configuration 

Fluorochromes:

  • Filter combinations for all usual fluorochromes are available
  • Green-yellow laser specially suited for red fluorescent proteins, TexasRed and PE tandem dyes.

 Special features:

  • The integration of the sorter into a Class II type safety cabinet combined with an additional aeorosol management provides optimal biosafety protection. The sorter is ideally suited for sorting of infectious material and of genetically modified cells of BSL 2.
  • Ceramic nozzles in 70 µm, 85 µm, 100 µm and 130 µm sizes are available
  • Index sorting

Becton-Dickinson FACSAria IIu ("Aria")

3 air-cooled solid state laser: 488nm, 633nm, 407nm;
12 parameters, 10 colors

download complete configuration

Fluorochromes:

  • Filter combinations for most of the usual fluorochromes are available (detection of PerCP possible)

Special features:

  • ceramic nozzle in 70µm, 85µm, 100µm and 130µm sizes are available
  • aerosol management system offers the opportunity to sort infectious material and e.g. genetically modified cells of BSL 2

Beckman-Coulter MoFlo XDP ("XDP")

4 air-cooled solid state laser: 488nm, 640nm, 405nm, 560nm;
12 parameters, 10 colors

download complete configuration

Fluorochromes:

  • Filter combinations for all usual fluorochromes are available (PerCP detection not possible).
  • The availability of a green-yellow laser specially suited for red fluorescent proteins, TexasRed and PE tandem dyes.

Special features:

  • Aerosol management system offers the opportunity to sort infectious material and e.g. genetically modified cells of BSL 2
  • Ceramic nozzle for many cell sizes: 70µm, 100µm, 150µm (more upon request),
    thereby specially suited for sorting large cells

Beckman-Coulter MoFlo XDP upgrade ("MoFlo")

3 lasers (solid state diode + Krypton ionen laser)
Laser 1: Coherent Sapphire 488-200 (488 nm)
Laser 2: Coherent Obis 405-100 (405 nm)
Laser 3: Coherent Innova 90K Krypton (647nm, 568nm, MLUV);
9 parameters, 7 colors

download complete configuration


Fluorochromes:

  • filter combinations for many usual fluorochromes are available (PerCP detection not possible)
  • applications requiring the excitation in UV range are possible, e.g. SP- cells stained with Hoechst dye;

Special features:

  • ceramic nozzle for many cell sizes: 70µm, 100µm, 150µm (more upon request), thereby specially suited for sorting large cells

10x Chromium Single Cell Controller

The 10x Chromium System offers numerous solutions for molecular analysis at the single cell level.

Further information on the technology can be found on the 10x Genomics website.

With the operation of the Chromium Single Cell Controller in the Central Research Facility Cell Sorting a fast processing of flow cytometrically sorted cell populations for single-cell analysis is guaranteed.

Please contact the Central Research Facility Genomics for further information.