Publikationen Publikationen LEBAO in press Schütze, T.M., Kolodziejczyk, A., Ditzer, N., Vangelisti, S., Capra, E., Lee, J., Chiaradia, I., Kufrin, V., Yamada, S., Peters, J., Krause, M., Eugster, C., Derihaci, R.P., Birdir, C., Namba, T., Wurm, A.A., Martin, U. , Wimberger, P., Long, K.R., Lancaster, M., Kalebic, N., Bonev, B., and Albert, M. 2026*. Human-specific morphoregulatory signatures in basal radial glia characterise neocortex evolution Nature Communications *in press. Szadocka, S., Teske, J., Franke, A., Mertens, M., Kriedemann, N., Witte, M., Drakhlis, L., Ruhparwar, A., Gruh, I., Zweigerdt, R. , and Andrée, B. 2026*. Structural and functional remodleling of large-scale cardiac tubes. Biofabrication . *in press. https://doi.org/10.1088/1758-5090/aeadd2 . 2026 Becker, F., Martins, S., Hernandez-Bautista, C. A., Greber, B., Zweigerdt, R. , and Kogler, G. 2026. Improved cryopreservation of cardiomyocyte aggregates differentiated from GMP iPSC in a 3D culture format. Sci Rep 16, no. 1:1640. https://doi.org/10.1038/s41598-025-32439-3 . Cyrys, K., Manstein, F., Triebert, W., Kriedemann, N., Hernandez-Bautista, C. A., and Zweigerdt, R. 2026. Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells. Nat Protoc . https://doi.org/10.1038/s41596-026-01398-y . Cyrys, K., and Zweigerdt, R. 2026. Scalable process technologies for human pluripotent stem cells (hPSCs) expansion and differentiation. Adv Drug Deliv Rev 235:115904. https://doi.org/10.1016/j.addr.2026.115904 . Dardano, M., Wilson, L., Zweigerdt, R. , and Drakhlis, L. 2026. Production of human blood-generating heart-forming organoids and sample preparation for advanced imaging. Nat Protoc 21, no. 7:2995. https://doi.org/10.1038/s41596-025-01268-z . De Boeck, K., Burgel, P. R., Bierlaagh, M., Hill, K., Amaral, M., Birimberg-Schwartz, L., Brewington, J. J., Davies, J. C., Karadag, B., Martin, U. , Mense, M., Pedemonte, N., Sharma, N., Tayor-Cousar, J. L., Sermet, I., and Beekman, J. M. 2026. ECFS statement on theratyping and theranostics in the context of rare and ultrarare CFTR variants in people with CF. J Cyst Fibros . https://doi.org/10.1016/j.jcf.2026.04.013 . Gruh, I., Martens, A., Cebotari, S., Schrod, A., Haase, A., Halloin, C., Triebert, W., Kriedemann, N., Goecke, T., Arar, M., Hoeffler, K., Frank, P., Lampe, K., Moussavi, A., Kuleshova, A., Hradil, V., Fricke, V., Szepes, M., Mätz-Rensing, K., Eiringhaus, J., de Vries, A. L., Hohmann, S., Witte, M., Kohrn, T., Teske, J., Cyrys, K., Szadocka, S., Franke, A., Werlein, C., Komorowski, K., Kühnel, M., Jonigk, D., Boretius, S., Veltmann, C., Duncker, D., Jung, J., Benvenisty, N., de la Roche, J., Fischer, M., Haverich, A., Ruhparwar, A., Zweigerdt, R. , and Martin, U. 2026. Human iPSC-cardiomyocyte-aggregate cell therapy in non-human primates and correlation of heart recovery with contractile and electrophysiological cardiomyocyte properties. Nat Commun 17, no. 1. https://doi.org/10.1038/s41467-026-77103-0 . Haase, A., Ruhparwar, A., and Martin, U. 2026. GMP-compliant manufacturing of iPSC-derived therapeutic cell products: Technologies, applications, risks and limitations. Adv Drug Deliv Rev 229:115744. https://doi.org/10.1016/j.addr.2025.115744 . Ivaskevica, K., Kowalski, K., Piep, B., Teske, J., Meissner, J. D., Kosanke, M., Radocaj, A., Montag, J., Zweigerdt, R. , Kraft, T., and Konze, S. A. 2026. Variable cMyBP-C expression from cell to cell in a MYBPC3(c.927-2 A>G) hiPSC-CM model recapitulates HCM patient phenotype. Stem Cell Res Ther 17, no. 1. https://doi.org/10.1186/s13287-026-05063-9 . Johansson, C., Kraus, Y., Sanchez, J. D. H., Schubert, B., Wolf, K., Voss, C. , Stoeger, T., Seegmüller, T., Flemmer, A. W., Förster, K., Standl, M., and Hilgendorff, A. 2026. Fine Particulate Exposure During Pregnancy Impacts on Perinatal Complications in Deeply Phenotyped Preterm Infants With Significant Immaturity. Pulm Circ 16, no. 2:e70320. https://doi.org/10.1002/pul2.70320 . Kiselev, E., Agyapong, W., Jürgens, B., Mohr, E., Chatterjee, S., Hunkler, H. J., Salman, J., Cipriano, G., Bentele, M., Liu, J., Specht, J., Menge, K. S., Waleczek, F. J. G., Haas, J. A., Derda, A. A., Sonnenschein, K., Gietz, A., Neumüller, S., Pfanne, A., Beetz, O., Pflaum, M., Wiegmann, B., Psaras, Y., Toepfer, C., Zweigerdt, R. , Radocaj, A., Kraft, T., Zeug, A., Ponimaskin, E., Korte, W., Horke, A., Ruhparwar, A., Fuchs, M., Xiao, K., Bär, C., Weber, N., and Thum, T. 2026. Transcriptional and functional effects of mavacamten in multiple porcine and human models with hypertrophic cardiomyopathy. Br J Pharmacol 183, no. 5:1122. https://doi.org/10.1111/bph.70247 . Kowalski, K., Haß, B., Montag, J., Meißner, J. D., Teske, J., Zweigerdt, R. , and Kraft, T. 2026. Cryopreservation alters contractile function of human induced pluripotent stem cell-derived cardiomyocytes. Sci Rep 16, no. 1. https://doi.org/10.1038/s41598-026-59927-4 . Leiber, L. M., Christian, L., Neubert, L., Ruwisch, J., Yilmaz, H., Plucinski, E. K. J., Langer, L. T., Kamp, J. C., Greer, M., Haermeyer, B., Kühnel, M., Werlein, C., Justet, A., Bergmann, A. K., Beeckmans, H., Ballmaier, M., Salman, J., Vos, R., Knudsen, L., Martin, U. , Vanaudenaerde, B. M., Yildirim, AÖ, Hohlfeld, J. M., Ius, F., Laenger, F., Welte, T., Falk, C., Kaminski, N., Jonigk, D. D., Gottlieb, J., and Schupp, J. C. 2026. Aberrant and Ectopic Cell Populations in Restrictive Allograft Syndrome after Lung Transplantation. Eur Respir J . https://doi.org/10.1183/13993003.00537-2025 . Ling, F., Şahin, A.T. , M. Naranjo, B., Aime, S., Roth, D., Pukaluk, A., Tepho, N., Augustin, R., Zampa, B., S. Vendrame, A., Klassen, M.-C. , Emken, E., Kiechle, M., Stoleriu, M.G. , Mertsch, P., Olmer, R. , Tesfaigzi, Y., Lieleg, O., and Nawroth, J.C. . 2026*. A General High-Throughput Mucus Microrheology Platform for Quantitative and Scalable Mucus Phenotyping. Advanced Science , no. e77750. https://doi.org/10.1002/advs.77750 . Maeding, N., Suresh Kundully, D., Steinhuber, A., Kriedemann, N., Hernandez-Bautista, C. A., Martins, S., Hochmann, S., Wolf, M., Mayr, W., Jungbauer, C., Diecke, S., Tonn, T., Greber, B., Zweigerdt, R. , and Strunk, D. 2026. Deep immune-phenotyping of HLA-homozygous iPS-cardiomyocytes by spectral flow cytometry. Front Immunol 17:1736994. https://doi.org/10.3389/fimmu.2026.1736994 . Metzger, S. M., Jones, T. C., Meier, J. I. J., Richter, A., Klassen, M. C., Olmer, R. , Diegmüller, N., Heimsch, K. C., and Drosten, C. 2026. Evaluation of a proposed link between the SARS-CoV-2 furin cleavage site and mouse-adapted MERS-coronavirus MA30. Proc Natl Acad Sci U S A 123, no. 27:e2601806123. https://doi.org/10.1073/pnas.2601806123 . Niehaus, I., Wilsch-Bräuninger, M., Mora-Bermúdez, F., Rost, F., Bobic-Rasonja, M., Radosevic, V., Milkovic-Perisa, M., Wimberger, P., Severino, M., Haase, A., Martin, U. , Kuenzel, K., Guan, K., Neumann, K., Walker, N., Schröck, E., Jovanov-Milosevic, N., Huttner, W. B., Di Donato, N., and Heide, M. 2026. Cerebral organoids expressing mutant actin genes reveal cellular mechanism underlying microcephaly. EMBO Rep 27, no. 2:387. https://doi.org/10.1038/s44319-025-00647-7 . Polten, F., Sandu, M. A., Faix, J., Hegermann, J., Kriedemann, N., Zweigerdt, R. , Thum, T., Bauersachs, J., Niessen, H. W., Koenig, A. L., Lavine, K. J., Pich, A., Wang, Y., Reboll, M. R., Korf-Klingebiel, M., and Wollert, K. C. 2026. Extracellular BRICK1 drives heart repair after myocardial infarction in mice. Sci Transl Med 18, no. 831:eadx2876. https://doi.org/10.1126/scitranslmed.adx2876 . Schwarz, M., Epe, B., Wohak, L. E., Voss, C. , and Hartwig, A. 2026. The role of cytotoxicity in the process of carcinogenesis. Arch Toxicol 100, no. 8:3791. https://doi.org/10.1007/s00204-026-04408-w . Voss, C. 2026. Rudolf Buchheim Award 2026: Toward a ToxAtlas of carbon-based nanomaterials: single-cell RNA sequencing reveals initiating cell circuits in pulmonary inflammation. Naunyn Schmiedebergs Arch Pharmacol . https://doi.org/10.1007/s00210-026-05170-7 . Voss, C. , Ryffel, B., and Stoeger, T. 2026. Respiratory toxicity of perfluorooctanesulfonic acid (PFOS) via Inflammasome involvement. Ecotoxicol Environ Saf 323:120730. https://doi.org/10.1016/j.ecoenv.2026.120730 . Wallman, D. J., Koc-Gunel, S., Hynds, R. E., Ryan, A. L., and Olmer, R. 2026. Advances in Human Lung Disease Models: Stem Cell-based Platforms for Modeling Airway and Alveolar Disease. Am J Physiol Lung Cell Mol Physiol . https://doi.org/10.1152/ajplung.00092.2026 . Wälzlein, J. H., Reusch, S., Ospina-Garcia, J., Olmer, R. , Schneider, M. A., Klotz, L. V., Klotz, C., and Kummer, S. 2026. Human airway organoids as a versatile model to study BSL-4 virus replication and pathogenesis. Sci Rep 16, no. 1. https://doi.org/10.1038/s41598-026-45813-6 . Waqas, F. H., Silva da Costa, L., Zapatero-Belinchón, F. J., Carter-Timofte, M. E., Lasswitz, L., van der Horst, D., Möller, R., Dahlmann, J., Olmer, R. , Geffers, R., Gerold, G., Olagnier, D., and Pessler, F. 2026. NRF2 activators and the inhibitor of nuclear export, selinexor, restrict coronaviruses by targeting a network involving ACE2, TMPRSS2, and XPO1 through an NRF2-independent mechanism. Commun Biol 9, no. 1. https://doi.org/10.1038/s42003-026-09724-6 . Xavier, A., Kolling, M., Bourke, A. M., Mosler, T., Prieto-Garcia, C., Karaulanov, E., Reissland, M., Tassetto, M., Kim, D., Klaassen, K., Grikscheit, K., Torbica, E., Dederer, V., Busam, J., Kazi, R., Olmer, R. , Pallerla, S. R., Mathea, S., Diefenbacher, M. E., Zarnack, K., Bojkova, D., Widera, M., Aviner, R., Andino, R., Ciesek, S., and Dikic, I. 2026. Programmed ribosomal frameshifting triggers translational stress to promote viral replication. Cell . https://doi.org/10.1016/j.cell.2026.08.031 . 2025 Dardano, M., Wilson, L., Zweigerdt, R ., Drakhlis L. Production of human blood-generating heart-forming organoids and sample preparation for advanced imaging. Nature Protocols 2025. https://doi.org/10.1038/s41596-025-01268-z Christoffersson, J., Kempf, H., Zweigerdt, R. , and Mandenius, C. F. 2025. Evaluating the Effect of Drug Compounds on Cardiac Spheroids Using the Cardiac Cell Outgrowth Assay in a Microphysiological System. Methods Mol Biol 2924:249. https://doi.org/10.1007/978-1-0716-4530-7_18 . Demkes, E., Cervera-Barea, A., Ebner-Peking, P., Wolf, M., Hochmann, S., Scheren, A. S., Bijsterveld, M., van Oostveen, C. M., Jansen, M., Visser, J., Triebert, W., Halloin, C., Dobbe, J. G. G., de Vos, J., Schürz, M., Danmayr, J., Aalders, M. C. G., Boink, G. J. J., Neef, K., Strunk, D., Zweigerdt, R. , de Jager, S. C. A., and Sluijter, J. P. G. 2025. Human Cardiac Microtissues Display Improved Engraftment and Survival in a Porcine Model of Myocardial Infarction. J Cardiovasc Transl Res . https://doi.org/10.1007/s12265-025-10596-0 . Haase, A., Ruhparwar, A., and Martin, U. 2025. GMP-compliant manufacturing of iPSC-derived therapeutic cell products: Technologies, applications, risks and limitations. Adv Drug Deliv Rev :115744. https://doi.org/10.1016/j.addr.2025.115744 . Hashemi, M., Finklea, F. B., Hammons, H., Tian, Y., Young, N., Kim, E., Halloin, C., Triebert, W., Zweigerdt, R. , Mitra, A. K., and Lipke, E. A. 2025. Hydrogel microsphere stem cell encapsulation enhances cardiomyocyte differentiation and functionality in scalable suspension system. Bioact Mater 43:423. https://doi.org/10.1016/j.bioactmat.2024.08.043 . Klassen, M. C., Balázs, A., Zöllner, J., Cleve, N., Czichon, L., von Schledorn, L., Hegermann, J., Nawroth, J. C., Roth, D., Mielenz, M., Hedtfeld, S., Stanke, F., Rubil, T., Ius, F., Jonigk, D., Hanrahan, J. W., Ruhparwar, A., Olmer, R. , Mall, M. A., Merkert, S., and Martin, U. 2025. Human induced pluripotent stem cells for in vitro modeling of impaired mucociliary clearance in cystic fibrosis lung disease. Stem Cell Res Ther 16, no. 1:573. https://doi.org/10.1186/s13287-025-04737-0 . Komorowski, K., Reichmann, J., Drakhlis, L. , Zweigerdt, R. , and Salditt, T. 2025. 3D histology of human heart-forming organoids by X-ray phase-contrast tomography. Commun Biol 8, no. 1:1411. https://doi.org/10.1038/s42003-025-08876-1 . Lynch-Miller, M., Lockow, S., Dümmer, K., Henneck, T., Olmer, R. , Jaboreck, M. C., Mergani, A. O., Wandrey, M., Branitzki-Heinemann, K., Brogden, G., Naim, H. Y., Martin, U. , Schulz, C., Talbot, S. R., Meurer, M., Baumgärtner, W., and von Köckritz-Blickwede, M. 2025. Characterization of 3D human pulmonary epithelial model morphology and oxygen status under normoxia and hypoxia. Biochim Biophys Acta Mol Cell Res 1872, no. 6:119980. https://doi.org/10.1016/j.bbamcr.2025.119980 . Manstein, F., Halloin, C., and Zweigerdt, R. 2025. Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors. Methods Mol Biol 2924:59. https://doi.org/10.1007/978-1-0716-4530-7_5 . Osten, F., Bodenschatz, A. K., Ivaskevica, K., Kröhn, S., Piep, B., Holler, T., Teske, J., Montag, J., Iorga, B., Weber, N., Zweigerdt, R. , Kraft, T., and Meissner, J. D. 2025. Differential impact of substrates on myosin heavy and light chain expression in human stem cell-derived cardiomyocytes at single-cell level. J Muscle Res Cell Motil . https://doi.org/10.1007/s10974-025-09690-2 . Roth, D., Şahin, A. T., Ling, F., Tepho, N., Senger, C. N., Quiroz, E. J., Calvert, B. A., van der Does, A. M., Güney, T. G., Glasl, S., van Schadewijk, A., von Schledorn, L., Olmer, R. , Kanso, E., Nawroth, J. C., and Ryan, A. L. 2025. Structure and function relationships of mucociliary clearance in human and rat airways. Nat Commun 16, no. 1:2446. https://doi.org/10.1038/s41467-025-57667-z . van der Does, A. M., L, V. Schledorn, and Olmer, R. 2025. Cell-based treatments of lung diseases: overview and outlook. Adv Drug Deliv Rev 227:115712. https://doi.org/10.1016/j.addr.2025.115712 . Voss, C. , Han, L., Ansari, M., Strunz, M., Haefner, V., Angelidis, I., Mayr, C. H., Berthing, T., Zhou, Q., Guenther, E. M., Huzain, O., Schmid, O., Vogel, U., Gote-Schniering, J., Gaedcke, S., Theis, F. J., Schiller, H. B., and Stoeger, T. 2025. Toward a ToxAtlas of Carbon-Based Nanomaterials: Single-Cell RNA Sequencing Reveals Initiating Cell Circuits in Pulmonary Inflammation. ACS Nano . https://doi.org/10.1021/acsnano.5c12054 . Weber, N., Montag, J., Kowalski, K., Iorga, B., de la Roche, J., Holler, T., Wojciechowski, D., Wendland, M., Radocaj, A., Mayer, A. K., Brunkhorst, A., Osten, F., Burkart, V., Piep, B., Bodenschatz, A., Gibron, P., Schwanke, K., Franke, A., Thiemann, S., Koroleva, A., Pfanne, A., Konsanke, M., Fiedler, J., Hegermann, J., Wrede, C., Mühlfeld, C., Chichkov, B., Fischer, M., Thum, T., Francino, A., Martin, U. , Meißner, J., Zweigerdt, R. , and Kraft, T. 2025. Patient-specific hiPSC-derived cardiomyocytes indicate allelic and contractile imbalance as pathogenic factor in early-stage Hypertrophic Cardiomyopathy. J Mol Cell Cardiol 198:112. https://doi.org/10.1016/j.yjmcc.2024.11.007 . 2024 Abdin, S. M., Mansel, F., Hashtchin, A. R., Ackermann, M., Hansen, G., Becker, B., Kick, B., Pham, N., Dietz, H., Schaniel, C., Martin, U. , Spreitzer, I., and Lachmann, N. 2024. Sensor macrophages derived from human induced pluripotent stem cells to assess pyrogenic contaminations in parenteral drugs. Biofabrication 16, no. 3. https://doi.org/10.1088/1758-5090/ad4744 . Andrée, B. , Voß, N., Kriedemann, N., Triebert, W., Teske, J., Mertens, M., Witte, M., Szádocka, S., Hilfiker, A. , Aper, T., Gruh, I. , and Zweigerdt, R. 2024. Fabrication of heart tubes from iPSC derived cardiomyocytes and human fibrinogen by rotating mold technology. Sci Rep 14, no. 1:13174. https://doi.org/10.1038/s41598-024-64022-7 . Bolesani, E., Bornhorst, D., Iyer, L. M., Zawada, D., Friese, N., Morgan, M., Lange, L., Gonzalez, D. M., Schrode, N., Leffler, A., Wunder, J., Franke, A., Drakhlis, L., Sebra, R., Schambach, A., Goedel, A., Dubois, N. C., Dobreva, G., Moretti, A., Zelaráyan, L. C., Abdelilah-Seyfried, S., and Zweigerdt, R. 2024. Transient stabilization of human cardiovascular progenitor cells from human pluripotent stem cells in vitro reflects stage-specific heart development in vivo. Cardiovasc Res 120, no. 11:1295. https://doi.org/10.1093/cvr/cvae118 . Cubillos, P., Ditzer, N., Kolodziejczyk, A., Schwenk, G., Hoffmann, J., Schütze, T. M., Derihaci, R. P., Birdir, C., Köllner, J. E., Petzold, A., Sarov, M., Martin, U. , Long, K. R., Wimberger, P., and Albert, M. 2024. The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex. Embo j 43, no. 8:1388. https://doi.org/10.1038/s44318-024-00068-7 . Dardano, M., Kleemiß, F., Kosanke, M., Lang, D., Wilson, L., Franke, A., Teske, J., Shivaraj, A., de la Roche, J., Fischer, M., Lange, L., Schambach, A., Drakhlis, L., and Zweigerdt, R. 2024. Blood-generating heart-forming organoids recapitulate co-development of the human haematopoietic system and the embryonic heart. Nat Cell Biol 26, no. 11:1984. https://doi.org/10.1038/s41556-024-01526-4 . Finklea, F. B., Hashemi, M., Tian, Y., Hammons, H., Halloin, C., Triebert, W., Zweigerdt, R. , and Lipke, E. A. 2024. Chemically defined production of engineered cardiac tissue microspheres from hydrogel-encapsulated pluripotent stem cells. Biotechnol Bioeng 121, no. 11:3614. https://doi.org/10.1002/bit.28818 . Goecke, T., Ius, F., Ruhparwar, A., and Martin, U. 2024. Unlocking the Future: Pluripotent Stem Cell-Based Lung Repair. Cells 13, no. 7. https://doi.org/10.3390/cells13070635 . Kriedemann, N., Manstein, F., Hernandez-Bautista, C. A., Ullmann, K., Triebert, W., Franke, A., Mertens, M., Stein, Icap, Leffler, A., Witte, M., Askurava, T., Fricke, V., Gruh, I. , Piep, B., Kowalski, K., Kraft, T., and Zweigerdt, R. 2024. Protein-free media for cardiac differentiation of hPSCs in 2000 mL suspension culture. Stem Cell Res Ther 15, no. 1:213. https://doi.org/10.1186/s13287-024-03826-w . Kriedemann, N., Triebert, W., Teske, J., Mertens, M., Franke, A., Ullmann, K., Manstein, F., Drakhlis, L., Haase, A., Halloin, C., Martin, U. , and Zweigerdt, R. 2024. Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks. Nat Protoc 19, no. 7:1911. https://doi.org/10.1038/s41596-024-00976-2 . Moenkemoeller, R., Budde, L., Martin, U. , Martens, A., and Seel, T. 2024. Towards Less Invasive Instruments For Cardiac Tissue Stabilizing. Current Directions in Biomedical Engineering , no. vol. 10, no. 4, 2024:pp. 445 ff. https://doi.org/10.1515/cdbme-2024-2109 . Neufeldt, D., Schmidt, A., Mohr, E., Lu, D., Chatterjee, S., Fuchs, M., Xiao, K., Pan, W., Cushman, S., Jahn, C., Juchem, M., Hunkler, H. J., Cipriano, G., Jürgens, B., Schmidt, K., Groß, S., Jung, M., Hoepfner, J., Weber, N., Foo, R., Pich, A., Zweigerdt, R. , Kraft, T., Thum, T., and Bär, C. 2024. Circular RNA circZFPM2 regulates cardiomyocyte hypertrophy and survival. Basic Res Cardiol 119, no. 4:613. https://doi.org/10.1007/s00395-024-01048-y . Rodriguez Gonzalez, C., Basílio-Queirós, D., Neehus, A. L., Merkert, S., Tschritter, D., Ünal, S., Hegermann, J., Mörgelin, M., Bustamante, J., Nietert, M. M., Martin, U. , Tümmler, B., Munder, A., and Lachmann, N. 2024. Human CFTR deficient iPSC-macrophages reveal impaired functional and transcriptomic response upon Pseudomonas aeruginosa infection. Front Immunol 15:1397886. https://doi.org/10.3389/fimmu.2024.1397886 . Schneider, A., Hage, A., Stein, Icap, Kriedemann, N., Zweigerdt, R. , and Leffler, A. 2024. A Possible Role of Tetrodotoxin-Sensitive Na(+) Channels for Oxidation-Induced Late Na(+) Currents in Cardiomyocytes. Int J Mol Sci 25, no. 12. https://doi.org/10.3390/ijms25126596 . Schukfeh, N., Sivaraman, K., Schmidt, A., Vieten, G., Dingemann, J., Weidner, J., Olmer, R. , and Janciauskiene, S. 2024. Alpha-1-antitrypsin improves anastomotic healing in intestinal epithelial cells model. Pediatr Surg Int 40, no. 1:258. https://doi.org/10.1007/s00383-024-05841-7 . Ullmann, K., Manstein, F., Triebert, W., Kriedemann, N., Franke, A., Teske, J., Mertens, M., Lupanow, V., Göhring, G., Haase, A., Martin, U. , and Zweigerdt, R. 2024. Matrix-free human pluripotent stem cell manufacturing by seed train approach and intermediate cryopreservation. Stem Cell Res Ther 15, no. 1:89. https://doi.org/10.1186/s13287-024-03699-z . 2023 Bojkova, D., Bechtel, M., Rothenburger, T., Kandler, J. D., Hayes, L., Olmer, R. , Martin, U. , Jonigk, D., Ciesek, S., Wass, M. N., Michaelis, M., and Cinatl, J., Jr. 2023. Omicron-induced interferon signaling prevents influenza A H1N1 and H5N1 virus infection. J Med Virol 95, no. 3:e28686. https://doi.org/10.1002/jmv.28686 . Bojkova, D., Reus, P., Panosch, L., Bechtel, M., Rothenburger, T., Kandler, J. D., Pfeiffer, A., Wagner, J. U. G., Shumliakivska, M., Dimmeler, S., Olmer, R. , Martin, U. , Vondran, F. W. R., Toptan, T., Rothweiler, F., Zehner, R., Rabenau, H. F., Osman, K. L., Pullan, S. T., Carroll, M. W., Stack, R., Ciesek, S., Wass, M. N., Michaelis, M., and Cinatl, J., Jr. 2023. Identification of novel antiviral drug candidates using an optimized SARS-CoV-2 phenotypic screening platform. iScience 26, no. 2:105944. https://doi.org/10.1016/j.isci.2023.105944 . Drakhlis, L., and Zweigerdt, R. 2023. Heart in a dish - choosing the right in vitro model. Dis Model Mech 16, no. 5. https://doi.org/10.1242/dmm.049961 . Eiringhaus, J., de Vries, A. L., Hohmann, S., Böthig, D., Müller-Leisse, J., Hillmann, H. A. K., Martens, A., Zweigerdt, R. , Schrod, A., Martin, U. , Duncker, D., Gruh, I. , and Veltmann, C. 2023. Performance and feasibility of three different approaches for computer based semi-automated analysis of ventricular arrhythmias in telemetric long-term ECG in cynomolgus monkeys. J Pharmacol Toxicol Methods 124:107471. https://doi.org/10.1016/j.vascn.2023.107471 . Harrison, S. P., Siller, R., Tanaka, Y., Chollet, M. E., de la Morena-Barrio, M. E., Xiang, Y., Patterson, B., Andersen, E., Bravo-Pérez, C., Kempf, H., Åsrud, K. S., Lunov, O., Dejneka, A., Mowinckel, M. C., Stavik, B., Sandset, P. M., Melum, E., Baumgarten, S., Bonanini, F., Kurek, D., Mathapati, S., Almaas, R., Sharma, K., Wilson, S. R., Skottvoll, F. S., Boger, I. C., Bogen, I. L., Nyman, T. A., Wu, J. J., Bezrouk, A., Cizkova, D., Corral, J., Mokry, J., Zweigerdt, R. , Park, I. H., and Sullivan, G. J. 2023. Scalable production of tissue-like vascularized liver organoids from human PSCs. Exp Mol Med 55, no. 9:2005. https://doi.org/10.1038/s12276-023-01074-1 . Heseding, H., Jahn, K., Brändl, B., Haase, A., Shum, I. O., Kohrn, T., Bleich, S., Frieling, H., Martin, U. , Müller, F. J., Wunderlich, S., and Deest, M. 2023. Generation of an induced pluripotent stem cell line, ZIPi021-A, from fibroblasts of a Prader-Willi syndrome patient with maternal uniparental disomy (mUPD). Stem Cell Res 71:103143. https://doi.org/10.1016/j.scr.2023.103143 . Junge, S., Ricci Signorini, M. E., Al Masri, M., Gülink, J., Brüning, H., Kasperek, L., Szepes, M., Bakar, M., Gruh, I. , Heisterkamp, A., and Torres-Mapa, M. L. 2023. A micro-LED array based platform for spatio-temporal optogenetic control of various cardiac models. Sci Rep 13, no. 1:19490. https://doi.org/10.1038/s41598-023-46149-1 . Lin, C., Kuffour, E. O., Fuchs, N. V., Gertzen, C. G. W., Kaiser, J., Hirschenberger, M., Tang, X., Xu, H. C., Michel, O., Tao, R., Haase, A., Martin, U. , Kurz, T., Drexler, I., Görg, B., Lang, P. A., Luedde, T., Sparrer, K. M. J., Gohlke, H., König, R., and Münk, C. 2023. Regulation of STING activity in DNA sensing by ISG15 modification. Cell Rep 42, no. 11:113277. https://doi.org/10.1016/j.celrep.2023.113277 . Merkert, S., Haase, A., Dahlmann, J., Göhring, G., Waqas, F. H., Pessler, F., Martin, U. #, and Olmer, R. #. 2023. Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing. Stem Cell Res 69:103090. https://doi.org/10.1016/j.scr.2023.103090 . Mhlekude, B., Postmus, D., Stenzel, S., Weiner, J., 3rd, Jansen, J., Zapatero-Belinchón, F. J., Olmer, R. , Richter, A., Heinze, J., Heinemann, N., Mühlemann, B., Schroeder, S., Jones, T. C., Müller, M. A., Drosten, C., Pich, A., Thiel, V., Martin, U. , Niemeyer, D., Gerold, G., Beule, D., and Goffinet, C. 2023. Pharmacological inhibition of bromodomain and extra-terminal proteins induces an NRF-2-mediated antiviral state that is subverted by SARS-CoV-2 infection. PLoS Pathog 19, no. 9:e1011657. https://doi.org/10.1371/journal.ppat.1011657 . Ori, C., Ansari, M., Angelidis, I., Olmer, R. , Martin, U. , Theis, F. J., Schiller, H. B., and Drukker, M. 2023. Human pluripotent stem cell fate trajectories toward lung and hepatocyte progenitors. iScience 26, no. 11:108205. https://doi.org/10.1016/j.isci.2023.108205 . Osten, F., Weber, N., Wendland, M., Holler, T., Piep, B., Kröhn, S., Teske, J., Bodenschatz, A. K., Devadas, S. B., Menge, K. S., Chatterjee, S., Schwanke, K., Kosanke, M., Montag, J., Thum, T., Zweigerdt, R. , Kraft, T., Iorga, B., and Meissner, J. D. 2023. Myosin expression and contractile function are altered by replating stem cell-derived cardiomyocytes. J Gen Physiol 155, no. 11. https://doi.org/10.1085/jgp.202313377 . Shum, I. O., Merkert, S., Malysheva, S., Jahn, K., Lachmann, N., Verboom, M., Frieling, H., Hallensleben, M., and Martin, U. 2023. An Improved Protocol for Targeted Differentiation of Primed Human Induced Pluripotent Stem Cells into HLA-G-Expressing Trophoblasts to Enable the Modeling of Placenta-Related Disorders. Cells 12, no. 16. https://doi.org/10.3390/cells12162070 . Staar, B. O., Hegermann, J., Auber, B., Ewen, R., von Hardenberg, S., Olmer, R. , Pink, I., Rademacher, J., Wetzke, M., and Ringshausen, F. C. 2023. Ciliary Ultrastructure Assessed by Transmission Electron Microscopy in Adults with Bronchiectasis and Suspected Primary Ciliary Dyskinesia but Inconclusive Genotype. Cells 12, no. 22. https://doi.org/10.3390/cells12222651 . Vivekanandan, R., Szepes, M., Ricci Signorini, M. E., Kravchenko, D., Kiefer, J., Berger, S., Fricke, V., Göhring, G., and Gruh, I. 2023. Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242. Stem Cell Res 66:102981. https://doi.org/10.1016/j.scr.2022.102981 . von Schledorn, L., Puertollano Martín, D., Cleve, N., Zöllner, J., Roth, D., Staar, B. O., Hegermann, J., Ringshausen, F. C., Nawroth, J., Martin, U. #, and Olmer, R. #. 2023. Primary Ciliary Dyskinesia Patient-Specific hiPSC-Derived Airway Epithelium in Air-Liquid Interface Culture Recapitulates Disease Specific Phenotypes In Vitro. Cells 12, no. 11. # authors contributed equally. https://doi.org/10.3390/cells12111467 . Waqas, F. H., Shehata, M., Elgaher, W. A. M., Lacour, A., Kurmasheva, N., Begnini, F., Kiib, A. E., Dahlmann, J., Chen, C., Pavlou, A., Poulsen, T. B., Merkert, S., Martin, U. , Olmer, R. , Olagnier, D., Hirsch, A. K. H., Pleschka, S., and Pessler, F. 2023. NRF2 activators inhibit influenza A virus replication by interfering with nucleo-cytoplasmic export of viral RNPs in an NRF2-independent manner. PLoS Pathog 19, no. 7:e1011506. * authors contributed equally. https://doi.org/10.1371/journal.ppat.1011506 . 2022 Ackermann, M., Rafiei Hashtchin, A., Manstein, F., Carvalho Oliveira, M., Kempf, H., Zweigerdt, R. , and Lachmann, N. 2022. Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors. Nat Protoc 17, no. 2:513. https://doi.org/10.1038/s41596-021-00654-7 . Gonzalez, D. M., Schrode, N., Ebrahim, T. A. M., Broguiere, N., Rossi, G., Drakhlis, L., Zweigerdt, R. , Lutolf, M. P., Beaumont, K. G., Sebra, R., and Dubois, N. C. 2022. Dissecting mechanisms of chamber-specific cardiac differentiation and its perturbation following retinoic acid exposure. Development 149, no. 13. https://doi.org/10.1242/dev.200557 . Jaboreck, M. C., Lühmann, J. L., Mielenz, M., Stanke, F., Göhring, G., Martin, U. , Olmer, R. #, and Merkert, S.#. 2022. Generation of two TMEM16A knockout iPSC clones each from a healthy human iPSC line, from a Cystic Fibrosis patient specific line with p.Phe508del mutation and from the gene corrected iPSC line. Stem Cell Res 64:102918. #authors contributed equally. https://doi.org/10.1016/j.scr.2022.102918 . Jaeger, B., Schupp, J. C., Plappert, L., Terwolbeck, O., Artysh, N., Kayser, G., Engelhard, P., Adams, T. S., Zweigerdt, R. , Kempf, H., Lienenklaus, S., Garrels, W., Nazarenko, I., Jonigk, D., Wygrecka, M., Klatt, D., Schambach, A., Kaminski, N., and Prasse, A. 2022. Airway basal cells show a dedifferentiated KRT17(high)Phenotype and promote fibrosis in idiopathic pulmonary fibrosis. Nat Commun 13, no. 1:5637. https://doi.org/10.1038/s41467-022-33193-0 . Kirchhoff, H., Ricke-Hoch, M., Wohlan, K., Pietzsch, S., Karsli, Ü, Erschow, S., Zweigerdt, R. , Ganser, A., Eder, M., Scherr, M., and Hilfiker-Kleiner, D. 2022. Chemotherapy-Free Targeted Anti-BCR-ABL+ Acute Lymphoblastic Leukemia Therapy May Benefit the Heart. Cancers (Basel) 14, no. 4. https://doi.org/10.3390/cancers14040983 . Lindner, M., Laporte, A., Elomaa, L., Lee-Thedieck, C., Olmer, R. , and Weinhart, M. 2022. Flow-induced glycocalyx formation and cell alignment of HUVECs compared to iPSC-derived ECs for tissue engineering applications. Front Cell Dev Biol 10:953062. https://doi.org/10.3389/fcell.2022.953062 . Malik, M. N. H., Waqas, S. F., Zeitvogel, J., Cheng, J., Geffers, R., Gouda, Z. A., Elsaman, A. M., Radwan, A. R., Schefzyk, M., Braubach, P., Auber, B., Olmer, R. , Müsken, M., Roesner, L. M., Gerold, G., Schuchardt, S., Merkert, S., Martin, U. , Meissner, F., Werfel, T., and Pessler, F. 2022. Congenital deficiency reveals critical role of ISG15 in skin homeostasis. J Clin Invest 132, no. 3. https://doi.org/10.1172/jci141573 . Niemeyer, D., Stenzel, S., Veith, T., Schroeder, S., Friedmann, K., Weege, F., Trimpert, J., Heinze, J., Richter, A., Jansen, J., Emanuel, J., Kazmierski, J., Pott, F., Jeworowski, L. M., Olmer, R. , Jaboreck, M. C., Tenner, B., Papies, J., Walper, F., Schmidt, M. L., Heinemann, N., Möncke-Buchner, E., Baumgardt, M., Hoffmann, K., Widera, M., Thao, T. T. N., Balázs, A., Schulze, J., Mache, C., Jones, T. C., Morkel, M., Ciesek, S., Hanitsch, L. G., Mall, M. A., Hocke, A. C., Thiel, V., Osterrieder, K., Wolff, T., Martin, U. , Corman, V. M., Müller, M. A., Goffinet, C., and Drosten, C. 2022. SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression. PLoS Biol 20, no. 11:e3001871. https://doi.org/10.1371/journal.pbio.3001871 . Oripov, F., Ramm, R., Falk, C., Goecke, T., Ebken, J., Jashari, R., Böthig, D., Horke, A., Avsar, M., Bobylev, D., Haverich, A., Hilfiker, A. , and Sarikouch, S. 2022. Serial assessment of early antibody binding to decellularized valved allografts. Front Cardiovasc Med 9:895943. https://doi.org/10.3389/fcvm.2022.895943 . Ricci Signorini, M. E., Szepes, M., Melchert, A., Bakar, M., Merkert, S., Haase, A., Göhring, G., Martin, U. , and Gruh, I. 2022. Generation of human induced pluripotent stem cell lines encoding for genetically encoded calcium indicators RCaMP1h and GCaMP6f. Stem Cell Res 60:102697. https://doi.org/10.1016/j.scr.2022.102697 . Schilling, T., Meyer, T., Brandes, G., Hartung, D., Tudorache, I., Nolte, I., Wacker, F., Hilfiker, A. , Hoeffler, K., Haverich, A., and Cebotari, S. 2022. Left Ventricular Wall Reconstruction with Autologous Vascularized Gastric Graft in a Porcine Pilot Model. Eur Surg Res . https://doi.org/10.1159/000522478 . van der Velden, J., Asselbergs, F. W., Bakkers, J., Batkai, S., Bertrand, L., Bezzina, C. R., Bot, I., Brundel, Bjjm, Carrier, L., Chamuleau, S., Ciccarelli, M., Dawson, D., Davidson, S. M., Dendorfer, A., Duncker, D. J., Eschenhagen, T., Fabritz, L., Falcão-Pires, I., Ferdinandy, P., Giacca, M., Girao, H., Gollmann-Tepeköylü, C., Gyongyosi, M., Guzik, T. J., Hamdani, N., Heymans, S., Hilfiker, A. , Hilfiker-Kleiner, D., Hoekstra, A. G., Hulot, J. S., Kuster, D. W. D., van Laake, L. W., Lecour, S., Leiner, T., Linke, W. A., Lumens, J., Lutgens, E., Madonna, R., Maegdefessel, L., Mayr, M., van der Meer, P., Passier, R., Perbellini, F., Perrino, C., Pesce, M., Priori, S., Remme, C. A., Rosenhahn, B., Schotten, U., Schulz, R., Sipido, K. R., Sluijter, J. P. G., van Steenbeek, F., Steffens, S., Terracciano, C. M., Tocchetti, C. G., Vlasman, P., Yeung, K. K., Zacchigna, S., Zwaagman, D., and Thum, T. 2022. Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC Working Group on Myocardial Function and the ESC Working Group on Cellular Biology of the Heart. Cardiovasc Res 118, no. 15:3016. https://doi.org/10.1093/cvr/cvab370 . Waqas, S. F., Sohail, A., Nguyen, A. H. H., Usman, A., Ludwig, T., Wegner, A., Malik, M. N. H., Schuchardt, S., Geffers, R., Winterhoff, M., Merkert, S., Martin, U. , Olmer, R. , Lachmann, N., and Pessler, F. 2022. ISG15 deficiency features a complex cellular phenotype that responds to treatment with itaconate and derivatives. Clin Transl Med 12, no. 7:e931. https://doi.org/10.1002/ctm2.931 . Wunderlich, S., Haase, A., Merkert, S., Jahn, K., Deest, M., Frieling, H., Glage, S., Korte, W., Martens, A., Kirschning, A., Zeug, A., Ponimaskin, E., Göhring, G., Ackermann, M., Lachmann, N., Moritz, T., Zweigerdt, R. , and Martin, U. 2022. Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies. Mol Ther Methods Clin Dev 26:84. https://doi.org/10.1016/j.omtm.2022.05.011 . 2021 Andrade, A. C., Wolf, M., Binder, H. M., Gomes, F. G., Manstein, F., Ebner-Peking, P., Poupardin, R., Zweigerdt, R. , Schallmoser, K., and Strunk, D. 2021. Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles. Int J Mol Sci 22, no. 8. https://doi.org/10.3390/ijms22083890 . Banerjee, S.*, Szepes, M.*, Dibbert, N., Rios-Camacho, J. C., Kirschning, A., Gruh, I. #, and Dräger, G.# 2021. Dextran-based scaffolds for in-situ hydrogelation: Use for next generation of bioartificial cardiac tissues. Carbohydr Polym 262:117924. */# authors contributed equally. https://doi.org/10.1016/j.carbpol.2021.117924 . Benito-Kwiecinski, S.*, Giandomenico, S. L.*, Sutcliffe, M., Riis, E. S., Freire-Pritchett, P., Kelava, I., Wunderlich, S., Martin, U. , Wray, G. A., McDole, K., and Lancaster, M. A. 2021. An early cell shape transition drives evolutionary expansion of the human forebrain. Cell 184, no. 8:2084. * authors contributed equally. https://doi.org/10.1016/j.cell.2021.02.050 . Chatterjee, S., Hofer, T., Costa, A., Lu, D., Batkai, S., Gupta, S. K., Bolesani, E., Zweigerdt, R. , Megias, D., Streckfuss-Bömeke, K., Brandenberger, C., Thum, T., and Bär, C. 2021. Telomerase therapy attenuates cardiotoxic effects of doxorubicin. Mol Ther 29, no. 4:1395. https://doi.org/10.1016/j.ymthe.2020.12.035 . Drakhlis, L., Biswanath, S., Farr, C. M., Lupanow, V., Teske, J., Ritzenhoff, K., Franke, A., Manstein, F., Bolesani, E., Kempf, H., Liebscher, S., Schenke-Layland, K., Hegermann, J., Nolte, L., Meyer, H., de la Roche, J., Thiemann, S., Wahl-Schott, C., Martin, U. , and Zweigerdt, R. 2021. Human heart-forming organoids recapitulate early heart and foregut development. Nat Biotechnol 39, no. 6:737. https://doi.org/10.1038/s41587-021-00815-9 . Drakhlis, L.*, Devadas, S. B.*, and Zweigerdt, R. 2021. Generation of heart-forming organoids from human pluripotent stem cells. Nat Protoc . * authors contributed equally. https://doi.org/10.1038/s41596-021-00629-8 . Ebken, J., Mester, N., Smart, I., Ramm, R., Goecke, T., Jashari, R., Böthig, D., Horke, A., Cebotari, S., Tudorache, I., Avsar, M., Bobylev, D., Haverich, A. , Sarikouch, S., and Hilfiker, A. 2021. Residual immune response towards decellularized homografts may be highly individual. Eur J Cardiothorac Surg 59, no. 4:773. https://doi.org/10.1093/ejcts/ezaa393 . Eggenschwiler, R., Gschwendtberger, T., Felski, C., Jahn, C., Langer, F., Sterneckert, J., Hermann, A., Lühmann, J., Steinemann, D., Haase, A., Martin, U. , Petri, S., and Cantz, T. 2021. A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines. Sci Rep 11, no. 1:22154. https://doi.org/10.1038/s41598-021-01689-2 . Eyre, K., Samper, E., Haverich, A. , Hilfiker, A. , and Andrée, B. 2021. Re-endothelialization of non-detergent decellularized porcine vessels. Artif Organs 45, no. 4:E53. https://doi.org/10.1111/aor.13836 . Haase, A., Kohrn, T., Fricke, V., Ricci Signorini, M. E., Witte, M., Göhring, G., Gruh, I. , and Martin, U. 2021. Establishment of MHHi001-A-5, a GCaMP6f and RedStar dual reporter human iPSC line for in vitro and in vivo characterization and in situ tracing of iPSC derivatives. Stem Cell Res 52:102206. https://doi.org/10.1016/j.scr.2021.102206 . Kosanke, M., Davenport, C., Szepes, M., Wiehlmann, L., Kohrn, T., Dorda, M., Gruber, J., Menge, K., Sievert, M., Melchert, A., Gruh, I. , Göhring, G., and Martin, U. 2021. iPSC culture expansion selects against putatively actionable mutations in the mitochondrial genome. Stem Cell Reports 16, no. 10:2488. https://doi.org/10.1016/j.stemcr.2021.08.016 . Kosanke, M., Osetek, K., Haase, A., Wiehlmann, L., Davenport, C., Schwarzer, A., Adams, F., Kleppa, M. J., Schambach, A., Merkert, S., Wunderlich, S., Menke, S., Dorda, M., and Martin, U. 2021. Reprogramming enriches for somatic cell clones with small-scale mutations in cancer-associated genes. Mol Ther . https://doi.org/10.1016/j.ymthe.2021.04.007 . Latham, S. L., Weiß, N., Schwanke, K., Thiel, C., Croucher, D. R., Zweigerdt, R. , Manstein, D. J., and Taft, M. H. 2020. Myosin-18B Regulates Higher-Order Organization of the Cardiac Sarcomere through Thin Filament Cross-Linking and Thick Filament Dynamics. Cell Rep 32, no. 9:108090. https://doi.org/10.1016/j.celrep.2020.108090 . Liu, D., Caliskan, S., Rashidfarokhi, B., Oldenhof, H., Jung, K., Sieme, H., Hilfiker, A. , and Wolkers, W. F. 2021. Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves. Sci Rep 11, no. 1:12299. https://doi.org/10.1038/s41598-021-91802-2 . Malik, M. N. H., Waqas, S. F. H., Zeitvogel, J., Cheng, J., Geffers, R., Gouda, Z. A., Elsaman, A. M., Radwan, A. R., Schefzyk, M., Braubach, P., Auber, B., Olmer, R. , Müsken, M., Roesner, L. M., Gerold, G., Schuchardt, S., Merkert, S., Martin, U. , Meissner, F., Werfel, T., and Pessler, F. 2021. Congenital deficiency reveals critical role of ISG15 in skin homeostasis. J Clin Invest . https://doi.org/10.1172/jci141573 . Manstein, F., Ullmann, K., Kropp, C., Halloin, C., Triebert, W., Franke, A., Farr, C. M., Sahabian, A., Haase, A., Breitkreuz, Y., Peitz, M., Brüstle, O., Kalies, S., Martin, U. , Olmer, R. , and Zweigerdt, R. 2021. High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling. Stem Cells Transl Med . https://doi.org/10.1002/sctm.20-0453 . Manstein, F., Ullmann, K., Triebert, W., and Zweigerdt, R. 2021. Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors. STAR Protoc 2, no. 4:100988. https://doi.org/10.1016/j.xpro.2021.100988 . Merkert, S., Wunderlich, S., Beier, J., Franke, A., Schwanke, K., Göhring, G., Kraft, T., Francino, A., Zweigerdt, R. , and Martin, U. 2021. Generation of two iPSC clones (MHHi021-A and MHHi021-B) from a patient with hypertrophic cardiomyopathy with p.Arg723Gly mutation in the MYH7 gene. Stem Cell Res 52:102208. https://doi.org/10.1016/j.scr.2021.102208 . Morticelli, L., Magdei, M., Tschalaki, N., Petersen, B., Haverich, A. , and Hilfiker, A. 2021. Generation of glycans depleted decellularized porcine pericardium, using digestive enzymatic supplements and enzymatic mixtures for food industry. Xenotransplantation :e12705. https://doi.org/10.1111/xen.12705 . Müller, D., Donath, S., Brückner, E. G., Biswanath Devadas, S., Daniel, F., Gentemann, L., Zweigerdt, R. , Heisterkamp, A., and Kalies, S. M. K. 2021. How Localized Z-Disc Damage Affects Force Generation and Gene Expression in Cardiomyocytes. Bioengineering (Basel) 8, no. 12. https://doi.org/10.3390/bioengineering8120213 . Pflaum, M., Dahlmann, J., Engels, L., Naghilouy-Hidaji, H., Adam, D., Zöllner, J., Otto, A., Schmeckebier, S., Martin, U. , Haverich, A., Olmer, R. , and Wiegmann, B. 2021. Towards Biohybrid Lung: Induced Pluripotent Stem Cell Derived Endothelial Cells as Clinically Relevant Cell Source for Biologization. Micromachines (Basel) 12, no. 8. https://doi.org/10.3390/mi12080981 . Polyak, A., Bankstahl, J. P., Besecke, K. F. W., Hozsa, C., Triebert, W., Pannem, R. R., Manstein, F., Borcholte, T., Furch, M., Zweigerdt, R. , Gieseler, R. K., Bengel, F. M., and Ross, T. L. 2021. Simplified (89)Zr-Labeling Protocol of Oxine (8-Hydroxyquinoline) Enabling Prolonged Tracking of Liposome-Based Nanomedicines and Cells. Pharmaceutics 13, no. 7. https://doi.org/10.3390/pharmaceutics13071097 . Rafiei Hashtchin, A., Fehlhaber, B., Hetzel, M., Manstein, F., Stalp, J. L., Glage, S., Abeln, M., Zweigerdt, R. , Munder, A., Viemann, D., Ackermann, M., and Lachmann, N. 2021. Human iPSC-derived macrophages for efficient Staphylococcus aureus clearance in a murine pulmonary infection model. Blood Adv . https://doi.org/10.1182/bloodadvances.2021004853 . Ricke-Hoch, M., Stelling, E., Lasswitz, L., Gunesch, A. P., Kasten, M., Zapatero-Belinchón, F. J., Brogden, G., Gerold, G., Pietschmann, T., Montiel, V., Balligand, J. L., Facciotti, F., Hirsch, E., Gausepohl, T., Elbahesh, H., Rimmelzwaan, G. F., Höfer, A., Kühnel, M. P., Jonigk, D., Eigendorf, J., Tegtbur, U., Mink, L., Scherr, M., Illig, T., Schambach, A., Pfeffer, T. J., Hilfiker, A. , Haverich, A. , and Hilfiker-Kleiner, D. 2021. Impaired immune response mediated by prostaglandin E2 promotes severe COVID-19 disease. PLoS One 16, no. 8:e0255335. https://doi.org/10.1371/journal.pone.0255335 . Sahabian, A.*, Dahlmann, J.*, Martin, U.# , and Olmer, R.# . 2021. Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions. Nat Protoc . */# authors contributed equally. https://doi.org/10.1038/s41596-020-00470-5 . Smart, I., Goecke, T., Ramm, R., Petersen, B., Lenz, D., Haverich, A. , Niemann, H., and Hilfiker, A. 2021. Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves. Xenotransplantation 28, no. 1:e12646. https://doi.org/10.1111/xen.12646 . Wolkers, W. F., and Hilfiker, A. 2021. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability. Methods Mol Biol 2180:731. https://doi.org/10.1007/978-1-0716-0783-1_40 . Publikationen 2016-2020 2020 Dahlmann, J., Sahabian, A., Drick, N., Haase, A., Göhring, G., Lachmann, N., Ringshausen, F. C., Welte, T., Martin, U. , and Olmer, R. 2020. Generation of two hiPSC lines (MHHi016-A, MHHi016-B) from a primary ciliary dyskinesia patient carrying a homozygous 5 bp duplication (c.248_252dup (p.Gly85Cysfs*11)) in exon 1 of the CCNO gene. Stem Cell Res 46:101850. https://doi.org/10.1016/j.scr.2020.101850 . Drick, N., Dahlmann, J., Sahabian, A., Haase, A., Göhring, G., Lachmann, N., Ringshausen, F. C., Welte, T., Martin, U. , and Olmer, R. 2020. Generation of two human induced pluripotent stem cell lines (MHHi017-A, MHHi017-B) from a patient with primary ciliary dyskinesia carrying a homozygous mutation (c.7915C > T [p.Arg2639*]) in the DNAH5 gene. Stem Cell Res 46:101848. https://doi.org/10.1016/j.scr.2020.101848 . Drick, N., Sahabian, A., Pongpamorn, P., Merkert, S., Göhring, G., Welte, T., Martin, U. , and Olmer, R. 2020. Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4). Stem Cell Res 42:101659. https://doi.org/10.1016/j.scr.2019.101659 . Findeisen, K., Morticelli, L., Goecke, T., Kolbeck, L., Ramm, R., Höffler, H. K., Brandes, G., Korossis, S., Haverich, A. , and Hilfiker, A. 2020. Toward acellular xenogeneic heart valve prostheses: Histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices. Xenotransplantation 27, no. 5:e12617. https://doi.org/10.1111/xen.12617 . Godehardt, A. W., Ramm, R., Gulich, B., Tönjes, R. R., and Hilfiker, A. 2020. Decellularized pig pulmonary heart valves-Depletion of nucleic acids measured by proviral PERV pol. Xenotransplantation 27, no. 2:e12565. https://doi.org/10.1111/xen.12565 . Hunkler, H. J., Hoepfner, J., Huang, C. K., Chatterjee, S., Jara-Avaca, M., Gruh, I. , Bolesani, E., Zweigerdt, R. , Thum, T., and Bär, C. 2020. The Long Non-coding RNA Cyrano Is Dispensable for Pluripotency of Murine and Human Pluripotent Stem Cells. Stem Cell Reports 15, no. 1:13. https://doi.org/10.1016/j.stemcr.2020.05.011 . Ichanti, H., Sladic, S., Kalies, S., Haverich, A., Andrée, B., and Hilfiker, A. 2020. Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels. Gels 6, no. 3. https://doi.org/10.3390/gels6030027 . Kreuser, U., Buchert, J., Haase, A., Richter, W., and Diederichs, S. 2020. Initial WNT/β-Catenin Activation Enhanced Mesoderm Commitment, Extracellular Matrix Expression, Cell Aggregation and Cartilage Tissue Yield From Induced Pluripotent Stem Cells. Front Cell Dev Biol 8:581331. https://doi.org/10.3389/fcell.2020.581331 . Lipus, A.*, Janosz, E.*, Ackermann, M., Hetzel, M., Dahlke, J., Buchegger, T., Wunderlich, S., Martin, U. , Cathomen, T., Schambach, A., Moritz, T.#, and Lachmann, N.#. 2020. Targeted Integration of Inducible Caspase-9 in Human iPSCs Allows Efficient in vitro Clearance of iPSCs and iPSC-Macrophages. Int J Mol Sci 21, no. 7. */# authors contributed equally. https://doi.org/10.3390/ijms21072481 . Merkert, S., Jaboreck, M. C., Engels, L., Malik, M. N. H., Göhring, G., Pessler, F., Martin, U. , and Olmer, R. 2020. Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing. Stem Cell Res 50:102135 https://doi.org/10.1016/j.scr.2020.102135 . Merkert, S., Schubert, M., Haase, A., Janssens, H. M., Scholte, B., Lachmann, N., Gohring, G., and Martin, U. 2020. Generation of an induced pluripotent stem cell line (MHHi018-A) from a patient with Cystic Fibrosis carrying p.Asn1303Lys (N1303K) mutation. Stem Cell Res 44:101744. https://doi.org/10.1016/j.scr.2020.101744 . Nowak-Imialek, M., Wunderlich, S., Herrmann, D., Breitschuh-Leibling, S., Gohring, G., Petersen, B., Klein, S., Baulain, U., Lucas-Hahn, A., Martin, U. , and Niemann, H. 2020. In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos. Cell Reprogram 22, no. 3:118. https://doi.org/10.1089/cell.2019.0107 . Pongpamorn, P.*, Dahlmann, J.*, Haase, A., Ebeling, C. T., Merkert, S., Gohring, G., Lachmann, N., Martens, A., Haverich, A., Martin, U.# , and Olmer, R. # . 2020. Generation of three induced pluripotent stem cell lines (MHHi012-A, MHHi013-A, MHHi014-A) from a family with Loeys-Dietz syndrome carrying a heterozygous p.M253I (c.759G>A) mutation in the TGFBR1 gene. Stem Cell Res 43:101707. */# authors contributed equally. https://doi.org/10.1016/j.scr.2020.101707 . Ramm, R., Goecke, T., Theodoridis, K., Hoeffler, K., Sarikouch, S., Findeisen, K., Ciubotaru, A., Cebotari, S., Tudorache, I., Haverich, A. , and Hilfiker, A. 2020. Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model. Xenotransplantation 27, no. 2:e12571. https://doi.org/10.1111/xen.12571 . Sahabian, A., von Schlehdorn, L., Drick, N., Pink, I., Dahlmann, J., Haase, A., Göhring, G., Welte, T., Martin, U., Ringshausen, F. C., and Olmer, R. 2020. Generation of two hiPSC clones (MHHi019-A, MHHi019-B) from a primary ciliary dyskinesia patient carrying a homozygous deletion in the NME5 gene (c.415delA (p.Ile139Tyrfs*8)). Stem Cell Res 48:101988. https://doi.org/10.1016/j.scr.2020.101988 . Stelling, E., Ricke-Hoch, M., Erschow, S., Hoffmann, S., Bergmann, A. K., Heimerl, M., Pietzsch, S., Battmer, K., Haase, A., Stapel, B., Scherr, M., Balligand, J. L., Binah, O., and Hilfiker-Kleiner, D. 2020. Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation. PLoS Biol 18, no. 12:e3000739. https://doi.org/10.1371/journal.pbio.3000739 . Szepes, M., Melchert, A., Dahlmann, J., Hegermann, J., Werlein, C., Jonigk, D., Haverich, A., Martin, U. , Olmer, R. , and Gruh, I. 2020. Dual Function of iPSC-Derived Pericyte-Like Cells in Vascularization and Fibrosis-Related Cardiac Tissue Remodeling In Vitro. Int J Mol Sci 21, no. 23. https://doi.org/10.3390/ijms21238947 . Taghizadeh, S., Jones, M. R., Olmer, R. , Ulrich, S., Danopoulos, S., Shen, C., Chen, C., Wilhelm, J., Martin, U. , Chen, C., Al Alam, D., and Bellusci, S. 2020. Fgf10 Signaling-Based Evidence for the Existence of an Embryonic Stage Distinct From the Pseudoglandular Stage During Mouse Lung Development. Front Cell Dev Biol 8:576604. https://doi.org/10.3389/fcell.2020.576604 . Weber, N.*, Kowalski, K.*, Holler, T., Radocaj, A., Fischer, M., Thiemann, S., de la Roche, J., Schwanke, K., Piep, B., Peschel, N., Krumm, U., Lingk, A., Wendland, M., Greten, S., Schmitto, J. D., Ismail, I., Warnecke, G., Zywietz, U., Chichkov, B., Meissner, J., Haverich, A., Martin, U. , Brenner, B., Zweigerdt, R. , and Kraft, T. 2020. Advanced Single-Cell Mapping Reveals that in hESC Cardiomyocytes Contraction Kinetics and Action Potential Are Independent of Myosin Isoform. Stem Cell Reports . * authors contributed equaly. https://doi.org/10.1016/j.stemcr.2020.03.015 . Williams, B., Löbel, W., Finklea, F., Halloin, C., Ritzenhoff, K., Manstein, F., Mohammadi, S., Hashemi, M., Zweigerdt, R. , Lipke, E., and Cremaschi, S. 2020. Prediction of Human Induced Pluripotent Stem Cell Cardiac Differentiation Outcome by Multifactorial Process Modeling. Front Bioeng Biotechnol 8:851. https://doi.org/10.3389/fbioe.2020.00851 . 2019 Andree, B. , Ichanti, H. , Kalies, S., Heisterkamp, A., Strauss, S., Vogt, P. M., Haverich, A. , and Hilfiker, A. 2019. Formation of three-dimensional tubular endothelial cell networks under defined serum-free cell culture conditions in human collagen hydrogels. Sci Rep 9, no. 1:5437. https://doi.org/10.1038/s41598-019-41985-6 . Christoffersson, J., Meier, F., Kempf, H., Schwanke, K. , Coffee, M. , Beilmann, M., Zweigerdt, R. , and Mandenius, C. F. 2019. Evaluating the Effect of Drug Compounds on Cardiac Spheroids Using the Cardiac Cell Outgrowth Assay. Methods Mol Biol 1994:185. https://doi.org/10.1007/978-1-4939-9477-9_17 . de la Roche, J., Angsutararux, P., Kempf, H., Janan, M., Bolesani, E., Thiemann, S., Wojciechowski, D., Coffee, M. , Franke, A. , Schwanke, K. , Leffler, A., Luanpitpong, S., Issaragrisil, S., Fischer, M., and Zweigerdt, R. 2019. Comparing human iPSC-cardiomyocytes versus HEK293T cells unveils disease-causing effects of Brugada mutation A735V of NaV1.5 sodium channels. Sci Rep 9, no. 1:11173. https://doi.org/10.1038/s41598-019-47632-4 . Eigendorf, J., Melk, A., Haufe, S., Boethig, D., Berliner, D., Kerling, A., Kueck, M., Stenner, H., Bara, C., Stiesch, M., Schippert, C., Hilfiker, A. , Falk, C., Bauersachs, J., Thum, T., Lichtinghagen, R., Haverich, A., Hilfiker-Kleiner, D., and Tegtbur, U. 2019. Effects of personalized endurance training on cellular age and vascular function in middle-aged sedentary women. Eur J Prev Cardiol :2047487319849505. https://doi.org/10.1177/2047487319849505 . Engels, L., Olmer, R. , de la Roche, J., Göhring, G., Ulrich, S., Haller, R., Martin, U.# , and Merkert, S.#. 2019. Generation of a CFTR knock-in reporter cell line (MHHi006-A-1) from a human induced pluripotent stem cell line. Stem Cell Res 40:101542. # authors contributed equally. https://doi.org/10.1016/j.scr.2019.101542 . Fiedler, J., Park, D. H., Hobuss, L., Anaraki, P. K., Pfanne, A., Just, A., Mitzka, S., Dumler, I., Weidemann, F., Hilfiker, A. , and Thum, T. 2019. Identification of miR-143 as a Major Contributor for Human Stenotic Aortic Valve Disease. J Cardiovasc Transl Res . https://doi.org/10.1007/s12265-019-09880-7 . Grund, A., Szaroszyk, M., Doppner, J. K., Malek Mohammadi, M., Kattih, B., Korf-Klingebiel, M., Gigina, A., Scherr, M., Kensah, G., Jara-Avaca, M., Gruh, I. , Martin, U. , Wollert, K. C., Gohla, A., Katus, H. A., Muller, O. J., Bauersachs, J., and Heineke, J. 2019. A gene therapeutic approach to inhibit calcium and integrin binding protein 1 ameliorates maladaptive remodelling in pressure overload. Cardiovasc Res 115, no. 1:71. https://doi.org/10.1093/cvr/cvy154 . Haase, A., Glienke, W., Engels, L., Gohring, G., Esser, R., Arseniev, L., and Martin, U. 2019. GMP-compatible manufacturing of three iPS cell lines from human peripheral blood. Stem Cell Res 35:101394. https://doi.org/10.1016/j.scr.2019.101394 . Halloin, C., Coffee, M., Manstein, F., and Zweigerdt, R. 2019. Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies. Methods Mol Biol 1994:55. https://doi.org/10.1007/978-1-4939-9477-9_5 . Halloin, C., Schwanke, K., Lobel, W., Franke, A., Szepes, M., Biswanath, S., Wunderlich, S., Merkert, S., Weber, N., Osten, F., de la Roche, J., Polten, F., Wollert, K., Kraft, T., Fischer, M., Martin, U. , Gruh, I. , Kempf, H., and Zweigerdt, R. 2019. Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture. Stem Cell Reports 13, no. 2:366. https://doi.org/10.1016/j.stemcr.2019.06.004 . Isu, G., Morbiducci, U., De Nisco, G., Kropp, C., Marsano, A., Deriu, M. A., Zweigerdt, R. , Audenino, A., and Massai, D. 2019. Modeling methodology for defining a priori the hydrodynamics of a dynamic suspension bioreactor. Application to human induced pluripotent stem cell culture. J Biomech . https://doi.org/10.1016/j.jbiomech.2019.07.021 . Manstein, F., Halloin, C., and Zweigerdt, R. 2019. Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors. Methods Mol Biol 1994:79. https://doi.org/10.1007/978-1-4939-9477-9_7 . Merkert, S.*, Schubert, M.*, Olmer, R.* , Engels, L., Radetzki, S., Veltman, M., Scholte, B. J., Zollner, J., Pedemonte, N., Galietta, L. J. V., von Kries, J. P., and Martin, U. 2019. High-Throughput Screening for Modulators of CFTR Activity Based on Genetically Engineered Cystic Fibrosis Disease-Specific iPSCs. Stem Cell Reports 12, no. 6:1389. * authors contributed equally. https://doi.org/10.1016/j.stemcr.2019.04.014 . Mogaldea, A., Theodoridis, K., Goecke, T., Tudorache, I., Haverich, A. , Cebotari, S., and Hilfiker, A. 2019. Assessment of cytocompatibility and mechanical properties of detergent-decellularized ovine pericardial tissue. Int J Artif Organs :391398819850583. https://doi.org/10.1177/0391398819850583 . Muller, D., Hagenah, D., Biswanath, S., Coffee, M., Kampmann, A., Zweigerdt, R. , Heisterkamp, A., and Kalies, S. M. K. 2019. Femtosecond laser-based nanosurgery reveals the endogenous regeneration of single Z-discs including physiological consequences for cardiomyocytes. Sci Rep 9, no. 1:3625. https://doi.org/10.1038/s41598-019-40308-z . Olmer, R. , Dahlmann, J., Merkert, S., Baus, S., Gohring, G., and Martin, U. 2019. Generation of a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-2). Stem Cell Res 39:101492. https://doi.org/10.1016/j.scr.2019.101492 . Sahabian, A., Sgodda, M., Naujok, O., Dettmer, R., Dahlmann, J., Manstein, F., Cantz, T., Zweigerdt, R. , Martin, U. , and Olmer, R. 2019. Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential. Cells 8, no. 12. https://doi.org/10.3390/cells8121571 . Sarikouch, S., Theodoridis, K., Hilfiker, A., Boethig, D., Laufer, G., Andreas, M., Cebotari, S., Tudorache, I., Bobylev, D., Neubert, L., Teiken, K., Robertus, J. L., Jonigk, D., Beerbaum, P., Haverich, A. , and Horke, A. 2019. Early Insight Into In Vivo Recellularization of Cell-Free Allogenic Heart Valves. Ann Thorac Surg 108, no. 2:581. https://doi.org/10.1016/j.athoracsur.2019.02.058 . Vasquez-Rivera, A., Oldenhof, H., Hilfiker, A. , and Wolkers, W. F. 2019. Spectral fingerprinting of decellularized heart valve scaffolds. Spectrochim Acta A Mol Biomol Spectrosc 214:95. https://doi.org/10.1016/j.saa.2019.02.006 . 2018 Ackermann, M.*, Kempf, H.*, Hetzel, M., Hesse, C., Hashtchin, A. R., Brinkert, K., Schott, J. W., Haake, K., Kuhnel, M. P., Glage, S., Figueiredo, C., Jonigk, D., Sewald, K., Schambach, A., Wronski, S., Moritz, T., Martin, U. , Zweigerdt, R. , Munder, A.#, and Lachmann, N.#. 2018. Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections. Nat Commun 9, no. 1:5088. */# authors contributed equally. https://doi.org/10.1038/s41467-018-07570-7 . Aper, T., Wilhelmi, M., Boer, U., Lau, S., Benecke, N., Hilfiker, A. , and Haverich, A. 2018. Dehydration improves biomechanical strength of bioartificial vascular graft material and allows its long-term storage. Innov Surg Sci 3, no. 3:215. https://doi.org/10.1515/iss-2018-0017 . Beckmann, E.*, Kensah, G.*, Neumann, A., Benecke, N., Martens, A., Martin, U. , Gruh, I. , and Haverich, A. 2018. Prolonged myocardial protection during hypothermic storage: potential application for cardiac surgery and myocardial tissue engineering. Biomedical Physics & Engineering Express 4, no. 3:035010. * authors contributed equally. http://stacks.iop.org/2057-1976/4/i=3/a=035010 Christoffersson, J., Meier, F., Kempf, H., Schwanke, K., Coffee, M., Beilmann, M., Zweigerdt, R. , and Mandenius, C. F. 2018. A Cardiac Cell Outgrowth Assay for Evaluating Drug Compounds Using a Cardiac Spheroid-on-a-Chip Device. Bioengineering (Basel) 5, no. 2. https://doi.org/10.3390/bioengineering5020036 . Eicke, D., Baigger, A., Schulze, K., Latham, S. L., Halloin, C., Zweigerdt, R. , Guzman, C. A., Blasczyk, R., and Figueiredo, C. 2018. Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors. Sci Rep 8, no. 1:10146. https://doi.org/10.1038/s41598-018-28459-x . Froese, N.*, Wang, H.*, Zwadlo, C.*, Wang, Y., Grund, A., Gigina, A., Hofmann, M., Kilian, K., Scharf, G., Korf-Klingebiel, M., Melchert, A., Ricci Signorini, M. E., Halloin, C., Zweigerdt, R. , Martin, U. , Gruh, I. , Wollert, K. C., Geffers, R., Bauersachs, J., and Heineke, J. 2018. Anti-androgenic therapy with finasteride improves cardiac function, attenuates remodeling and reverts pathologic gene-expression after myocardial infarction in mice. J Mol Cell Cardiol 122:114. * authors contributed equally. https://doi.org/10.1016/j.yjmcc.2018.08.011 . Gaspari, E., Franke, A., Robles-Diaz, D., Zweigerdt, R. , Roeder, I., Zerjatke, T.#, and Kempf, H.#. 2018. Paracrine mechanisms in early differentiation of human pluripotent stem cells: Insights from a mathematical model. Stem Cell Res 32:1. # authors contributed equally. https://doi.org/10.1016/j.scr.2018.07.025 . Gieseler, G. M., Ekramzadeh, K., Nolle, V., Malysheva, S., Kempf, H., Beutel, S., Zweigerdt, R. , Martin, U. , Rinas, U., Scheper, T., and Pepelanova, I. 2018. Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies. Biotechnol Rep (Amst) 18:e00249. https://doi.org/10.1016/j.btre.2018.e00249 . Goecke, T., Theodoridis, K., Tudorache, I., Ciubotaru, A., Cebotari, S., Ramm, R., Höffler, K., Sarikouch, S., Vásquez Rivera, A., Haverich, A. , Wolkers, W. F., and Hilfiker, A. 2018. In vivo performance of freeze-dried decellularized pulmonary heart valve allo- and xenografts orthotopically implanted into juvenile sheep. Acta Biomater 68:41. https://doi.org/10.1016/j.actbio.2017.11.041 . Hainz, N.*, Beckmann, A.*, Schubert, M., Haase, A., Martin, U. , Tschernig, T., and Meier, C. 2018. Human stem cells express pannexins. BMC Res Notes 11, no. 1:54. *authors contributed equally. https://doi.org/10.1186/s13104-018-3125-z . Harrison, P. T., Hoppe, N., and Martin, U. 2018. Gene editing & stem cells. J Cyst Fibros 17, no. 1:10. https://doi.org/10.1016/j.jcf.2017.11.018 . Iorga, B. , Schwanke, K., Weber, N., Wendland, M., Greten, S., Piep, Birgit, dos Remedios, C. G., Martin, U. , Zweigerdt, R. , Kraft, T., and Brenner, B. 2018. Differences in Contractile Function of Myofibrils within Human Embryonic Stem Cell-Derived Cardiomyocytes vs. Adult Ventricular Myofibrils Are Related to Distinct Sarcomeric Protein Isoforms. Frontiers in Physiology 8, no. 1111. https://doi.org/10.3389/fphys.2017.01111 . Jara Avaca, M., and Gruh, I. 2018. Bioengineered Cardiac Tissue Based on Human Stem Cells for Clinical Application. Adv Biochem Eng Biotechnol 163:117. https://link.springer.com/content/pdf/10.1007%2F10_2017_24.pdf . Kempf, H., and Zweigerdt, R. 2018. Scalable Cardiac Differentiation of Pluripotent Stem Cells Using Specific Growth. Adv Biochem Eng Biotechnol 163:39. https://link.springer.com/content/pdf/10.1007%2F10_2017_30.pdf . Koch, L.*, Deiwick, A.*, Franke, A., Schwanke, K., Haverich, A. , Zweigerdt, R.# , and Chichkov, B.#. 2018. Laser bioprinting of human induced pluripotent stem cells-the effect of printing and biomaterials on cell survival, pluripotency, and differentiation. Biofabrication 10, no. 3:035005. */# authors contributed equally. https://doi.org/10.1088/1758-5090/aab981 . Lipps, C.*, Klein, F.*, Wahlicht, T.*, Seiffert, V., Butueva, M., Zauers, J., Truschel, T., Luckner, M., Koster, M., MacLeod, R., Pezoldt, J., Huhn, J., Yuan, Q., Muller, P. P., Kempf, H., Zweigerdt, R. , Dittrich-Breiholz, O., Pufe, T., Beckmann, R., Drescher, W., Riancho, J., Sanudo, C., Korff, T., Opalka, B., Rebmann, V., Gothert, J. R., Alves, P. M., Ott, M., Schucht, R., Hauser, H., Wirth, D., and May, T. 2018. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9, no. 1:994. *Authors contributed equally. https://doi.org/10.1038/s41467-018-03408-4 . Malysheva, S. V., Wunderlich, S., Haase, A., Gohring, G., Martin, U.* , and Merkert, S.*. 2018. Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation. Stem Cell Res 30:117. *authors contributed equally. https://doi.org/10.1016/j.scr.2018.05.015 . Manikowski, D.*, Andree, B.*, Samper, E., Saint-Marc, C., Olmer, R. , Vogt, P., Strauss, S., Haverich, A. , and Hilfiker, A. 2018. Human adipose tissue-derived stromal cells in combination with exogenous stimuli facilitate three-dimensional network formation of human endothelial cells derived from various sources. Vascul Pharmacol 106:28. *authors contributed equally. https://doi.org/10.1016/j.vph.2018.02.003 . Merkert, S., and Martin, U. 2018. Targeted Gene Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases. Adv Biochem Eng Biotechnol 163:169. https://doi.org/10.1016/j.scr.2016.02.027 . Neehus, A. L.*, Lam, J.*, Haake, K., Merkert, S., Schmidt, N., Mucci, A., Ackermann, M., Schubert, M., Happle, C., Kuhnel, M. P., Blank, P., Philipp, F., Goethe, R., Jonigk, D., Martin, U. , Kalinke, U., Baumann, U., Schambach, A., Roesler, J., and Lachmann, N. 2018. Impaired IFNgamma-Signaling and Mycobacterial Clearance in IFNgammaR1-Deficient Human iPSC-Derived Macrophages. Stem Cell Reports 10, no. 1:7. *authors contributed equally. https://doi.org/10.1016/j.stemcr.2017.11.011 . Olmer, R.* , Engels, L.*, Usman, A., Menke, S., Malik, M. N. H., Pessler, F., Gohring, G., Bornhorst, D., Bolten, S., Abdelilah-Seyfried, S., Scheper, T., Kempf, H., Zweigerdt, R. , and Martin, U. 2018. Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture. Stem Cell Reports 10, no. 5:1657. *authors contributed equally. https://doi.org/10.1016/j.stemcr.2018.03.017 . Pamies, D., Bal-Price, A., Chesne, C., Coecke, S., Dinnyes, A., Eskes, C., Grillari, R., Gstraunthaler, G., Hartung, T., Jennings, P., Leist, M., Martin, U. , Passier, R., Schwamborn, J. C., Stacey, G. N., Ellinger-Ziegelbauer, H., and Daneshian, M. 2018. Advanced Good Cell Culture Practice for human primary, stem cell-derived and organoid models as well as microphysiological systems. ALTEX 35, no. 3:353. https://doi.org/10.14573/altex.1710081 . Taghizadeh, S., Kheirolahi, V., Snoeck, H. W., Olmer, R. , Martin, U. , and Bellusci, S. 2018. Investigating role of FGF10 in differentiation of human lung alvelolar epithelial cells using human ESCs and hiPSCs. Pneumologie 72, no. 03:234. https://doi.org/10.1055/s-0037-1615342 . Weist, R., Flörkemeier, T., Roger, Y., Franke, A., Schwanke, K., Zweigerdt, R. , U., Martin. , Willbold, E.#, and Hoffmann, A.#. 2018. Differential expression of cholinergic system components in human induced pluripotent stem cells, bone marrow‐derived multipotent stromal cells, and induced pluripotent stem cell-derived multipotent stromal cells. Stem Cells and Development 27, no. 3:166. # authors contributed equally. https://doi.org/10.1089/scd.2017.0162 . Wolling, H., Konze, S. A., Hofer, A., Erdmann, J., Pich, A., Zweigerdt, R. , and Buettner, F. F. R. 2018. Quantitative Secretomics Reveals Extrinsic Signals Involved in Human Pluripotent Stem Cell Cardiomyogenesis. Proteomics 18, no. 14:e1800102. https://doi.org/10.1002/pmic.201800102 . 2017 Ackermann, M.*, Kuhn, A.*, Kunkiel, J., Merkert, S., Martin, U. , Moritz, T., and Lachmann, N. 2017. Ex vivo Generation of Genetically Modified Macrophages from Human Induced Pluripotent Stem Cells. Transfus Med Hemother 44, no. 3:135. * authors contributed equally. https://doi.org/10.1159/000477129 . Gentemann, L.*, Kalies, S.*, Coffee, M., Meyer, H., Ripken, T., Heisterkamp, A., Zweigerdt, R. , and Heinemann, D. 2017. Modulation of cardiomyocyte activity using pulsed laser irradiated gold nanoparticles. Biomed Opt Express 8, no. 1:177. * authors contributed equally. https://doi.org/10.1364/boe.8.000177 . Haase, A., Göhring, G, and Martin, U. 2017. Generation of non-transgenic iPS cells from human cord blood CD34+ cells under animal component-free conditions. Stem Cell Research 21:71. https://doi.org/10.1016/j.scr.2017.03.022 . Jara-Avaca, M.*, Kempf, H.*, Ruckert, M., Robles-Diaz, D., Franke, A., de la Roche, J., Fischer, M., Malan, D., Sasse, P., Solodenko, W., Drager, G., Kirschning, A., Martin, U.# , and Zweigerdt, R.# . 2017. EBIO Does Not Induce Cardiomyogenesis in Human Pluripotent Stem Cells but Modulates Cardiac Subtype Enrichment by Lineage-Selective Survival. Stem Cell Reports 8, no. 2:305. */# authors contributed equally. https://doi.org/10.1016/j.stemcr.2016.12.012 . Konze, S. A., Werneburg, S., Oberbeck, A., Olmer, R. , Kempf, H., Jara-Avaca, M., Pich, A., Zweigerdt, R. , and Buettner, F. F. 2017. Proteomic Analysis of Human Pluripotent Stem Cell Cardiomyogenesis Revealed Altered Expression of Metabolic Enzymes and PDLIM5 Isoforms. J Proteome Res 16, no. 3:1133. https://doi.org/10.1021/acs.jproteome.6b00534 . Martin, U. 2017. Genome stability of programmed stem cell products. Adv Drug Deliv Rev 120:108. https://doi.org/10.1016/j.addr.2017.09.004 . Martin, U. 2017. Therapeutic Application of Pluripotent Stem Cells: Challenges and Risks. Front Med (Lausanne) 4:229. https://doi.org/10.3389/fmed.2017.00229 . Martin, U. , and Haverich, A. 2017. Myocardial tissue engineering. From basic research to clinical application. Zeitschrift für Herz-, Thorax- und Gefäßchirurgie 31, no. 3:200. https://doi.org/10.1007/s00398-016-0119-9 . Massai, D., Bolesani, E., Diaz, D. R., Kropp, C., Kempf, H., Halloin, C., Martin, U. , Braniste, T., Isu, G., Harms, V., Morbiducci, U., Drager, G., and Zweigerdt, R. 2017. Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: considerations on process development. Sci Rep 7, no. 1:3950. https://doi.org/10.1038/s41598-017-04158-x . Merkert, S., Bednarski, C, Göhring, G., Cathomen, T., and Martin, U. 2017. Generation of a gene-corrected isogenic control iPSC line from Cystic Fibrosis-patient specific iPSCs homozygous for p.Phe508del mutation mediated by TALENs and ssODN. Stem Cell Research 23:95. https://doi.org/10.1016/j.scr.2017.07.010 . Reboll, M. R.*, Korf-Klingebiel, M.*, Klede, S., Polten, F., Brinkmann, E., Reimann, I., Schonfeld, H. J., Bobadilla, M., Faix, J., Kensah, G., Gruh, I. , Klintschar, M., Gaestel, M., Niessen, H. W., Pich, A., Bauersachs, J., Gogos, J. A., Wang, Y., and Wollert, K. C. 2017. EMC10 (Endoplasmic Reticulum Membrane Protein Complex Subunit 10) Is a Bone Marrow-Derived Angiogenic Growth Factor Promoting Tissue Repair After Myocardial Infarction. Circulation 136, no. 19:1809. * authors contributed equally. https://doi.org/10.1161/circulationaha.117.029980 . Rojas, S. V., Kensah, G., Rotaermel, A., Baraki, H., Kutschka, I., Zweigerdt, R. , Martin, U. , Haverich, A. , Gruh, I.# , and Martens, A.#. 2017. Transplantation of purified iPSC-derived cardiomyocytes in myocardial infarction. PLoS One 12, no. 5:e0173222. #Authors contributed equally. https://doi.org/10.1371/journal.pone.0173222 . Rojas, S. V., Meier, M., Zweigerdt, R. , Eckardt, D., Rathert, C., Schecker, N., Schmitto, J. D., Rojas-Hernandez, S., Martin, U. , Kutschka, I., Haverich, A. , and Martens, A. 2017. Multimodal Imaging for In Vivo Evaluation of Induced Pluripotent Stem Cells in a Murine Model of Heart Failure. Artif Organs 41, no. 2:192. https://doi.org/10.1111/aor.12728 . Sgodda, M., Dai, Z., Zweigerdt, R. , Sharma, A. D., Ott, M., and Cantz, T. 2017. A Scalable Approach for the Generation of Human Pluripotent Stem Cell-Derived Hepatic Organoids with Sensitive Hepatotoxicity Features. Stem Cells Dev 26, no. 20:1490. https://doi.org/10.1089/scd.2017.0023 . Stanslowsky, N., Jahn, K., Venneri, A., Naujock, M., Haase, A., Martin, U. , Frieling, H., and Wegner, F. 2017. Functional effects of cannabinoids during dopaminergic specification of human neural precursors derived from induced pluripotent stem cells. Addict Biol 22, no. 5:1329. https://doi.org/10.1111/adb.12394 . 2016 Andrée, B., and Zweigerdt, R. 2016. Directing Cardiomyogenic Differentiation and Transdifferentiation By Ectopic Gene Expression - Direct Transition Or Reprogramming Detour? Curr Gene Ther 16, no. 1:14. https://doi.org/10.2174/1566523216666160104141522 . Beckmann, A., Schubert, M., Hainz, N., Haase, A., Martin, U. , Tschernig, T., and Meier, C. 2016. Ultrastructural demonstration of Cx43 gap junctions in induced pluripotent stem cells from human cord blood. Histochem Cell Biol 146, no. 5:529. https://doi.org/10.1007/s00418-016-1469-9 . Borger, A. K., Eicke, D., Wolf, C., Gras, C., Aufderbeck, S., Schulze, K., Engels, L., Eiz-Vesper, B., Schambach, A., Guzman, C. A., Lachmann, N., Moritz, T., Martin, U. , Blasczyk, R., and Figueiredo, C. 2016. Generation of HLA-universal iPSCs-derived megakaryocytes and platelets for survival under refractoriness conditions. Mol Med 22:274. https://doi.org/10.2119/molmed.2015.00235 . Christoffersson, J., Bergstrom, G., Schwanke, K., Kempf, H., Zweigerdt, R. , and Mandenius, C. F. 2016. A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies. Methods Mol Biol 1502:159. https://doi.org/10.1007/7651_2016_340 . Dibbert, N., Krause, A., Rios-Camacho, J. C., Gruh, I. , Kirschning, A., and Drager, G. 2016. A Synthetic Toolbox for the In Situ Formation of Functionalized Homo- and Heteropolysaccharide-Based Hydrogel Libraries. Chemistry 22, no. 52:18777. https://doi.org/10.1002/chem.201603748 . Kempf, H., Andree, B., and Zweigerdt, R. 2016. Large-scale production of human pluripotent stem cell derived cardiomyocytes. Adv Drug Deliv Rev 96:18. https://doi.org/10.1016/j.addr.2015.11.016 . Kempf, H., Olmer, R. , Haase, A., Franke, A., Bolesani, E., Schwanke, K., Robles-Diaz, D., Coffee, M., Gohring, G., Drager, G., Potz, O., Joos, T., Martinez-Hackert, E., Haverich, A., Buettner, F. F., Martin, U. , and Zweigerdt, R. 2016. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. Nat Commun 7:13602. https://doi.org/10.1038/ncomms13602 . Klawitter, S., Fuchs, N. V., Upton, K. R., Munoz-Lopez, M., Shukla, R., Wang, J., Garcia-Canadas, M., Lopez-Ruiz, C., Gerhardt, D. J., Sebe, A., Grabundzija, I., Merkert, S., Gerdes, P., Pulgarin, J. A., Bock, A., Held, U., Witthuhn, A., Haase, A., Sarkadi, B., Lower, J., Wolvetang, E. J., Martin, U., Ivics, Z., Izsvak, Z., Garcia-Perez, J. L., Faulkner, G. J., and Schumann, G. G. 2016. Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells. Nat Commun 7:10286. https://doi.org/10.1038/ncomms10286 . Kropp, C., Kempf, H., Halloin, C., Robles-Diaz, D., Franke, A., Scheper, T., Kinast, K., Knorpp, T., Joos, T. O., Haverich, A. , Martin, U. , Zweigerdt, R. , and Olmer, R . 2016. Impact of Feeding Strategies on the Scalable Expansion of Human Pluripotent Stem Cells in Single-Use Stirred Tank Bioreactors. Stem Cells Transl Med 5, no. 10:1289. https://doi.org/10.5966/sctm.2015-0253 . Merkert, S., and Martin, U. 2016. Targeted genome engineering using designer nucleases: State of the art and practical guidance for application in human pluripotent stem cells. Stem Cell Res 16, no. 2:377. https://doi.org/10.1016/j.scr.2016.02.027 . Merkert, S., and Martin, U. 2016. Site-Specific Genome Engineering in Human Pluripotent Stem Cells. Int J Mol Sci 17, no. 7. https://doi.org/10.3390/ijms17071000 . Viereck, J.*, Kumarswamy, R.*, Foinquinos, A.*, Xiao, K., Avramopoulos, P., Kunz, M., Dittrich, M., Maetzig, T., Zimmer, K., Remke, J., Just, A., Fendrich, J., Scherf, K., Bolesani, E., Schambach, A., Weidemann, F., Zweigerdt, R., de Windt, L. J., Engelhardt, S., Dandekar, T., Batkai, S., and Thum, T. 2016. Long noncoding RNA Chast promotes cardiac remodeling. Sci Transl Med 8, no. 326:326ra22. * authors contributed equally. https://doi.org/10.1126/scitranslmed.aaf1475 . Weber, N., Schwanke, K., Greten, S., Wendland, M., Iorga, B., Fischer, M., Geers-Knorr, C., Hegermann, J., Wrede, C., Fiedler, J., Kempf, H., Franke, A., Piep, B., Pfanne, A., Thum, T., Martin, U. , Brenner, B., Zweigerdt, R. , and Kraft, T. 2016. Stiff matrix induces switch to pure beta-cardiac myosin heavy chain expression in human ESC-derived cardiomyocytes. Basic Res Cardiol 111, no. 6:68. https://doi.org/10.1007/s00395-016-0587-9 . Publikationen 2011 - 2015 2015 Bergstrom, G.*, Christoffersson, J.*, Schwanke, K., Zweigerdt, R., and Mandenius, C. F. 2015. Stem cell derived in vivo-like human cardiac bodies in a microfluidic device for toxicity testing by beating frequency imaging. Lab Chip 15, no. 15:3242. *Authors contributed equally. Effenberg, A.*, Stanslowsky, N.*, Klein, A., Wesemann, M., Haase, A., Martin, U., Dengler, R., Grothe, C., Ratzka, A.#, and Wegner, F.#. 2015. Striatal Transplantation of Human Dopaminergic Neurons Differentiated From Induced Pluripotent Stem Cells Derived From Umbilical Cord Blood Using Lentiviral Reprogramming. Cell Transplant 24, no. 10:2099. *#Authors contributed equally. Katsirntaki, K., Mauritz, C., Olmer, R., Schmeckebier, S., Sgodda, M., Puppe, V., Eggenschwiler, R., Duerr, J., Schubert, S. C., Schmiedl, A., Ochs, M., Cantz, T., Salwig, I., Szibor, M., Braun, T., Rathert, C., Martens, A., Mall, M. A., and Martin, U. 2015. Bronchoalveolar Sublineage Specification of Pluripotent Stem Cells: Effect of Dexamethasone Plus cAMP-Elevating Agents and Keratinocyte Growth Factor. Tissue Eng Part A 21, no. 3-4:669. Kempf, H.*, Kropp, C.*, Olmer, R., Martin, U., and Zweigerdt, R. 2015. Cardiac differentiation of human pluripotent stem cells in scalable suspension culture. Nat Protoc 10, no. 9:1345. *Authors contributed equally. Lachmann, N.*, Brennig, S.*, Hillje, R., Schermeier, H., Phaltane, R., Dahlmann, J., Gruh, I., Heinz, N., Schiedlmeier, B., Baum, C., and Moritz, T. 2015. Tightly regulated 'all-in-one' lentiviral vectors for protection of human hematopoietic cells from anticancer chemotherapy. Gene Ther 22, no. 11:883. *Authors contributed equally. Lachmann, N.*, Czarnecki, K.*, Brennig, S., Phaltane, R., Heise, M., Heinz, N., Kempf, H., Dilloo, D., Kaever, V., Schambach, A., Heuser, M., and Moritz, T. 2015. Deoxycytidine-kinase (dCK) knock-down as a novel myeloprotective strategy in the context of fludarabine, cytarabine, or cladribine therapy. Leukemia 29, no. 11:2266. *Authors contributed equally. Martin, U. 2015. New muscle for old hearts: engineering tissue from pluripotent stem cells. Hum Gene Ther 26, no. 5:305. Martin, U. 2015. Pluripotent Stem Cells for Disease Modelling and Drug Screening: New Perspectives for Treatment of Cystic Fibrosis? Molecular and Cellular Pediatrics 2, no. 1:15. Rojas, S. V.*, Martens, A.*, Zweigerdt, R., Baraki, H., Rathert, C., Schecker, N., Rojas-Hernandez, S., Schwanke, K., Martin, U., Haverich, A., and Kutschka, I. 2015. Transplantation effectiveness of induced pluripotent stem cells is improved by a fibrinogen biomatrix in an experimental model of ischemic heart failure. Tissue Eng Part A 21, no. 13-14:1991. *Authors contributed equally. 2014 Ackermann, M., Lachmann, N., Hartung, S., Eggenschwiler, R., Pfaff, N., Happle, C., Mucci, A., Gohring, G., Niemann, H., Hansen, G., Schambach, A., Cantz, T., Zweigerdt, R., and Moritz, T. 2014. Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based gene therapy. Biomaterials 35, no. 5:1531. Andree, B., Bela, K., Horvath, T., Lux, M., Ramm, R., Venturini, L., Ciubotaru, A., Zweigerdt, R., Haverich, A., and Hilfiker, A. 2014. Successful re-endothelialization of a perfusable biological vascularized matrix (BioVaM) for the generation of 3D artificial cardiac tissue. Basic Res Cardiol 109, no. 6:441. Beekman, J. M., Sermet-Gaudelus, I., de Boeck, K., Gonska, T., Derichs, N., Mall, M. A., Mehta, A., Martin, U., Drumm, M., and Amaral, M. D. 2014. CFTR functional measurements in human models for diagnosis, prognosis and personalized therapy: Report on the pre-conference meeting to the 11th ECFS Basic Science Conference, Malta, 26-29 March 2014. J Cyst Fibros 13, no. 4:363. Konze, S. A., van Diepen, L., Schroder, A., Olmer, R., Moller, H., Pich, A., Weissmann, R., Kuss, A. W., Zweigerdt, R., and Buettner, F. F. 2014. Cleavage of E-cadherin and beta-catenin by calpain affects Wnt signaling and spheroid formation in suspension cultures of human pluripotent stem cells. Mol Cell Proteomics 13, no. 4:990. Kempf, H.*, Olmer, R.*, Kropp, C., Ruckert, M., Jara-Avaca, M., Robles-Diaz, D., Franke, A., Elliott, D. A., Wojciechowski, D., Fischer, M., Roa Lara, A., Kensah, G., Gruh, I., Haverich, A., Martin, U., and Zweigerdt, R. 2014. Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture. Stem Cell Reports 3, no. 6:1132. *Authors contributed equally. Lachmann, N.*, Happle, C.*, Ackermann, M., Luttge, D., Wetzke, M., Merkert, S., Hetzel, M., Kensah, G., Jara-Avaca, M., Mucci, A., Skuljec, J., Dittrich, A.M., Pfaff, N., Brennig, S., Schambach, A., Steinemann, D., Gohring, G., Cantz, T., Martin, U., Schwerk, N., Hansen, G.#, and Moritz, T#. 2014. Gene correction of human induced pluripotent stem cells repairs the cellular phenotype in pulmonary alveolar proteinosis. Am J Respir Crit Care Med 189, no. 2:167. * #Authors contributed equally. Martens, A., Rojas, S.V., Baraki, H., Rathert, C., Schecker, N., Zweigerdt, R., Schwanke, K., Rojas-Hernandez, S., Martin, U., Saito, S., Schmitto, J.D., Haverich, A., and Kutschka, I. 2014. Substantial Early Loss of Induced Pluripotent Stem Cells Following Transplantation in Myocardial Infarction. Artif Organs 38, no. 11:978. Martens, A.*, Rojas, S. V.*, Baraki, H., Rathert, C., Schecker, N., Hernandez, S. R., Schwanke, K., Zweigerdt, R., Martin, U., Saito, S., Haverich, A., and Kutschka, I. 2014. Macroscopic fluorescence imaging: a novel technique to monitor retention and distribution of injected microspheres in an experimental model of ischemic heart failure. PLoS One 9, no. 8:e101775. *Authors contributed equally. Martin, U., and Haverich, A. 2014. Engineering cardiac muscle: new ways to refurbish old hearts? Eur J Cardiothorac Surg 45, no. 2:216. Merkert, S., Wunderlich, S., Bednarksi, C., Beier, J., Haase, A., Dreyer, A.-K., Schwanke, K., Meyer, J., Göhring, G., Cathomen, T., and Martin, U. 2014. Efficient designer nuclease-based homologous recombination enables direct PCR screening for footprint less targeted human pluripotent stem cell clones. Stem Cell Reports 2, no. 1:107. Naujock, M., Stanslowsky, N., Reinhardt, P., Sterneckert, J., Haase, A., Martin, U., Kim, K. S., Dengler, R., Wegner, F.#, and Petri, S.#. 2014. Molecular and functional analyses of motor neurons generated from human cord-blood-derived induced pluripotent stem cells. Stem Cells Dev 23, no. 24:3011. #Authors contributed equally. Schwanke, K., Merkert, S., Kempf, H., Hartung, S., Jara-Avaca, M., Templin, C., Gohring, G., Haverich, A., Martin, U.#, and Zweigerdt, R.#. 2014. Fast and efficient multitransgenic modification of human pluripotent stem cells. Hum Gene Ther Methods 25, no. 2:136. #Authors contributed equally. Stanslowsky, N., Haase, A., Martin, U., Naujock, M., Leffler, A., Dengler, R., and Wegner, F. 2014. Functional differentiation of midbrain neurons from human cord blood-derived induced pluripotent stem cells. Stem Cell Res Ther 5, no. 2:35. Wunderlich, S.*, Kircher, M.*, Vieth, B., Haase, A., Merkert, S., Beier, J., Göhring, G., Glage, S., Schambach, A., Curnow, E.C., Pääbo, S., Martin, U.#, and Enard, W.#. 2014. Primate iPS cells as tools for evolutionary analyses. Stem Cell Research 12:622. * #Authors contributed equally. Zweigerdt, R.*, Gruh, I.*, and Martin, U.*. 2014. Your Heart on a Chip: iPSC-Based Modeling of Barth-Syndrome-Associated Cardiomyopathy. Cell Stem Cell 15, no. 1:9. *Authors contributed equally. 2013 Buta, C., David, R., Dressel, R., Emgard, M., Fuchs, C., Gross, U., Healy, L., Hescheler, J., Kolar, R., Martin, U., Mikkers, H., Müller, F., Schneider, R., Seiler, A.E.M., Spielmann, H., and Weitzer, G. 2013. Reconsidering Pluripotency Tests: Do we still need Teratoma Assays?. Stem Cell Research 11:552. Dahlmann, J.*, Kensah, G.*, Kempf, H., Skvorc, D., Gawol, A., Elliott, D. A., Drager, G., Zweigerdt, R., Martin, U., and Gruh, I. 2013. The use of agarose microwells for scalable embryoid body formation and cardiac differentiation of human and murine pluripotent stem cells. Biomaterials 34, no. 10:2463. *Authors contributed equally. Dahlmann, J.*, Krause, A.*, Möller, L., Kensah, G., Möwes, M., Diekmann, A., Martin, U., Kirschning, A., Gruh, I.#, and Dräger, G.#. 2013. Fully defined in situ cross-linkable alginate and hyaluronic acid hydrogels for myocardial tissue engineering. Biomaterials 34, no. 4:490. *#Authors contributed equally. Emmert, M.Y., Emmert, L.S., Martens, A., Ismail, I., Schmidt-Richter, I., Gawol, A., Seifert, B., Haverich, A., Martin, U., and Gruh, I. 2013. Higher frequencies of BCRP+ cardiac resident cells in ischaemic human myocardium. Eur Heart J 34, no. 36:2830. Hartung, S., Schwanke, K., David, R., Franz, W.-M., Martin, U.*, and Zweigerdt, R.*. 2013. Directing cardiomyogenic differentiation of human induced pluripotent stem cells by plasmid-based transient overexpression of cardiac transcription factors. Stem Cells and Development 22, no. 7:1112. *Authors contributed equally. Kensah, G.*, Roa Lara, A.*, Dahlmann, J., Zweigerdt, R., Schwanke, K., Hegermann, J., Skvorc, D., Gawol, A., Azizian, A., Wagner, S., Maier, L., Krause, A., Dräger, G., Ochs, M., Haverich, A., Gruh, I.#, and Martin, U.#. 2013. Murine and human pluripotent stem cell-derived cardiac bodies form contractile myocardial tissue in vitro. Eur Heart J 34, no. 15:1134. *#Authors contributed equally. Kues, W., Herrmann, D., Barg-Kues, B., Meena Haridoss, S., Nowak-Imialek, M., Buchholz, T., Streeck, M., Grebe, A., Grabundzija, I., Merkert, S., Martin, U., Hall, V., Rasmussen, M., Ivics, Z., Maddox-Hyttel, P., and Niemann, H. 2013. Derivation and characterization of Sleeping Beauty transposon-mediated porcine induced pluripotent stem cells. Stem Cells Dev 22, no. 1:124. Lachmann, N., Brennig, S., Pfaff, N., Schermeier, H., Dahlmann, J., Phaltane, R., Gruh, I., Modlich, U., Schambach, A., Baum, C., and Moritz, T. 2013. Efficient in vivo regulation of cytidine deaminase expression in the haematopoietic system using a doxycycline-inducible lentiviral vector system. Gene Ther 20, no. 3:298. Schmeckebier, S.*, Mauritz, C.*, Katsirntaki, K., Sgodda, M., Puppe, V., Duerr, J., Schubert, S., Schmiedl, A., Lin, Q., Palecek, J., Draeger, G., Ochs, M., Zenke, M., Cantz, T., Mall, M., and Martin, U. 2013. Keratinocyte growth factor and -dexamethasone plus elevated cAMP levels synergistically support pluripotent stem cell differentiation into alveolar epithelial type II cells. Tissue Eng Part A 19, no. 7-8:938. *Authors contributed equally. 2012 Chen, R., John, J., Lavrentieva, A., Mueller, S., Tomala, M., Zhao, Y., Zweigerdt, R., Beutel, S., Hitzmann, B., Kasper, C., Martin, U., Rinas, U., Stahl, F., and Scheper, T. 2012. Cytokine production using membrane adsorbers: Human basic fibroblast growth factor produced by Escherichia coli. Eng Life Sci 12, no.1:29. Fiechter, M., Ghadri, J. R., Sidler, M., Martin, U., Landmesser, U., Kaufmann, P. A., Luscher, T. F., and Templin, C. 2012. Cardiac quadruple-fusion imaging: A brief report on a novel integrated multimodality approach for in vivo visualization of transplanted stem cells. Int J Cardiol 161, no. 1:62. Hochedlinger, K., Takahashi, K., Plath, K., Ng, H. H., Clark, A., Martin, U., Hanna, J. H., Deng, H., Silva, J.C.R., Woltjen, K., Boyer, L., and Wernig, M. 2012. Reprogramming: The Next Generation. Cell Stem Cell 11, no. 6:740. Martin, U. 2012. Induced pluripotent stem cells from blood. In Regenerative Therapy Using Blood-Derived Stem Cells. D.S. Allan and D. Strunk, editors. Humana Press. 87. Olmer, R., Lange, A., Selzer, S., Kasper, C., Haverich, A., Martin, U.*, and Zweigerdt, R.*. 2012. Suspension Culture of Human Pluripotent Stem Cells in Controlled, Stirred Bioreactors. Tissue Eng Part C Methods 18, no. 10:772. *Authors contributed equally. Templin, C., and Martin, U. 2012. Nichtinvasiver Nachweis transplantierter iPSCs. Laborwelt 4:22. Templin, C., Zweigerdt, R., Schwanke, K., Olmer, R., Ghadri, J. R., Emmert, M. Y., Muller, E., Kuest, S. M., Cohrs, S., Schibli, R., Kronen, P., Hilbe, M., Reinisch, A., Strunk, D., Haverich, A., Hoerstrup, S., Luscher, T. F., Kaufmann, P. A., Landmesser, U.*, and Martin, U.*. 2012. Transplantation and tracking of human-induced pluripotent stem cells in a pig model of myocardial infarction: assessment of cell survival, engraftment, and distribution by hybrid single photon emission computed tomography/computed tomography of sodium iodide symporter transgene expression. Circulation 126, no. 4:430. *Authors contributed equally. Wunderlich, S., Haase, A., Merkert, S., Beier, J., Schwanke, K., Schambach, A., Glage, S., Göhring, G., Curnow, E., and Martin, U. 2012. Induction of pluripotent stem cells from cynomolgus monkey using a polycistronic SIV-based vector, differentiation towards functional cardiomyocytes and generation of stably expressing reporter lines. Cell Reprogram 14, no. 6:471. 2011 Fiedler, J., Jazbutyte, V., Kirchmaier, B. C., Gupta, S. K., Lorenzen, J., Hartmann, D., Galuppo, P., Kneitz, S., Pena, J. T., Sohn-Lee, C., Loyer, X., Soutschek, J., Brand, T., Tuschl, T., Heineke, J., Martin, U., Schulte-Merker, S., Ertl, G., Engelhardt, S., Bauersachs, J., and Thum, T. 2011. MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124, no. 6:720. Grolms, M., Olmer, R., Martin, U., and Zweigerdt, R. 2011. Facilitating scale up:controlled stem cell cultivation in stirred suspension bioreactors. Biotech International 19. Kempf, H., Lecina, M., Ting, S., Zweigerdt, R., and Oh, S. 2011. Distinct regulation of mitogen-activated protein kinase activities is coupled with enhanced cardiac differentiation of human embryonic stem cells. Stem Cell Res 7, no. 3:198. Kensah, G., Gruh, I., Viering, J., Schumann, H., Dahlmann, J., Meyer, H., Skvorc, D., Bar, A., Akhyari, P., Heisterkamp, A., Haverich, A., and Martin, U. 2011. A novel miniaturized multimodal bioreactor for continuous in situ assessment of bioartificial cardiac tissue during stimulation and maturation. Tissue Eng Part C Methods 17, no. 4:463. Kütemeyer, K.*, Kensah, G.*, Heidrich, M., Meyer, H., Martin, U., Gruh, I.#, and Heisterkamp, A.#. 2011. Two-photon induced collagen cross-linking in bioartificial cardiac tissue. Optics Express 19, no. 17:15996. * #Authors contributed equally. Martens, A.*, Gruh, I.*, Dimitroulis, D., Rojas, S. V., Schmidt-Richter, I., Rathert, C., Khaladj, N., Gawol, A., Chikobava, M. G., Martin, U., Haverich, A., and Kutschka, I. 2011. Rhesus monkey cardiosphere-derived cells for myocardial restoration. Cytotherapy 13, no. 7:864. *Authors contributed equally. Mauritz, C.*, Martens, A.*, Rojas, S. V., Schnick, T., Rathert, C., Schecker, N., Menke, S., Glage, S., Zweigerdt, R., Haverich, A., Martin, U.#, and Kutschka, I.#. 2011. Induced pluripotent stem cell (iPSC)-derived Flk-1 progenitor cells engraft, differentiate, and improve heart function in a mouse model of acute myocardial infarction. Eur Heart J 32, no. 21:2634. *#Authors contributed equally. Moller, L., Krause, A., Dahlmann, J., Gruh, I., Kirschning, A., and Drager, G. 2011. Preparation and evaluation of hydrogel-composites from methacrylated hyaluronic acid, alginate, and gelatin for tissue engineering. Int J Artif Organs 34, no. 2:93. Palecek, J., Zweigerdt, R., Olmer, R., Martin, U., Kirschning, A., and Drager, G. 2011. A practical synthesis of Rho-Kinase inhibitor Y-27632 and fluoro derivatives and their evaluation in human pluripotent stem cells. Org Biomol Chem 9, no. 15:5503. Zweigerdt, R.*, Olmer, R.*, Singh, H., Haverich, A., and Martin, U. 2011. Scalable expansion of human pluripotent stem cells in suspension culture. Nat Protoc 6, no. 5:689. *Authors contributed equally. Publikationen 1998 - 2010 2010 Cantz, T., and Martin, U. 2010. Induced pluripotent stem cells: characteristics and perspectives. Adv Biochem Eng Biotechnol 123:107. Hess, C., Wiegmann, B., Maurer, A. N., Fischer, P., Moller, L., Martin, U., Hilfiker, A., Haverich, A., and Fischer, S. 2010. Reduced thrombocyte adhesion to endothelialized poly 4-methyl-1-pentene gas exchange membranes-a first step toward bioartificial lung development. Tissue Eng Part A 16, no. 10:3043. Mauritz, C., and Martin, U. 2010. Embryonic Stem Cells: Differentiation into Respiratory Cell Derivatives. In Stem cells: organogenesis and cancer, vol. 37/661 (2). S.R. Singh, editor. Transworld Research Network. Olmer, R., Haase, A., Merkert, S., Cui, W., Palecek, J., Ran, C., Kirschning, A., Scheper, T., Glage, S., Miller, K., Curnow, E. C., Hayes, E. S., and Martin, U. 2010. Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium. Stem Cell Res 5, no. 1:51. Ozkan, J., and Martin, U. 2010. In Vitro Generation of Large Blood Vessels Is the Key Technology for the Generation and Implantation of Larger Dimension Tissue. Circulation 121, no. 13:F76. Singh, H., Mok, P., Balakrishnan, T., Rahmat, S. N., and Zweigerdt, R. 2010. Up-scaling single cell-inoculated suspension culture of human embryonic stem cells. Stem Cell Res 4, no. 3:165. Tomala, M., Lavrentieva, A., Moretti, P., Rinas, U., Kasper, C., Stahl, F., Schambach, A., Warlich, E., Martin, U., Cantz, T., and Scheper, T. 2010. Preparation of bioactive soluble human leukemia inhibitory factor from recombinant Escherichia coli using thioredoxin as fusion partner. Protein Expr Purif 73, no. 1:51. Wernet, P., Trapp, T., Zweigerdt, R., Mann, J., and Trompeter, H. I. 2010. Lentiviral labeling reveals three germ layer differentiation potential of a single unrestricted somatic stem cell from human cord blood. Exp Hematol 38, no. 11:1099 2009 Ghodsizad, A., Niehaus, M., Kogler, G., Martin, U., Wernet, P., Bara, C., Khaladj, N., Loos, A., Makoui, M., Thiele, J., Mengel, M., Karck, M., Klein, H. M., Haverich, A., and Ruhparwar, A. 2009. Transplanted human cord blood-derived unrestricted somatic stem cells improve left-ventricular function and prevent left-ventricular dilation and scar formation after acute myocardial infarction. Heart 95, no. 1:27. Gruh, I., and Martin, U. 2009. Transdifferentiation of Stem Cells: A Critical View. Adv Biochem Eng Biotechnol 114:73. Haase, A., Olmer, R., Schwanke, K., Wunderlich, S., Merkert, S., Hess, C., Zweigerdt, R., Gruh, I., Meyer, J., Wagner, S., Maier, L. S., Han, D. W., Glage, S., Miller, K., Fischer, P., Scholer, H. R., and Martin, U. 2009. Generation of induced pluripotent stem cells from human cord blood. Cell Stem Cell 5, no. 4:434. Rust, W., Balakrishnan, T., and Zweigerdt, R. 2009. Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression. Regen Med 4, no. 2:225. Seewald, M. J., Ellinghaus, P., Kassner, A., Stork, I., Barg, M., Niebrugge, S., Golz, S., Summer, H., Zweigerdt, R., Schrader, E. M., Feicht, S., Jaquet, K., Reis, S., Korfer, R., and Milting, H. 2009. Genomic profiling of developing cardiomyocytes from recombinant murine embryonic stem cells reveals regulation of transcription factor clusters. Physiol Genomics 38, no. 1:7. Xaymardan, M., Cimini, M., Fazel, S., Weisel, R. D., Lu, W. Y., Martin, U., Harvey, R. P., and Li, R. K. 2009. c-Kit Function Is Necessary for In Vitro Myogenic Differentiation of Bone Marrow Hematopoietic Cells. Stem Cells 27, no. 8:1911. Xu, X. Q., Soo, S. Y., Sun, W., and Zweigerdt, R. 2009. Global expression profile of highly enriched cardiomyocytes derived from human embryonic stem cells. Stem Cells 27, no. 9:2163. Zweigerdt, R. 2009. Large scale production of stem cells and their derivatives. Adv Biochem Eng Biotechnol 114:201. 2008 Freund, C., Ward-van Oostwaard, D., Monshouwer-Kloots, J., van den Brink, S., van Rooijen, M., Xu, X., Zweigerdt, R., Mummery, C., and Passier, R. 2008. Insulin redirects differentiation from cardiogenic mesoderm and endoderm to neuroectoderm in differentiating human embryonic stem cells. Stem Cells 26, no. 3:724. Graichen, R., Xu, X., Braam, S. R., Balakrishnan, T., Norfiza, S., Sieh, S., Soo, S. Y., Tham, S. C., Mummery, C., Colman, A., Zweigerdt, R., and Davidson, B. P. 2008. Enhanced cardiomyogenesis of human embryonic stem cells by a small molecular inhibitor of p38 MAPK. Differentiation 76, no. 4:357. Gruh, I., Wunderlich, S., Winkler, M., Schwanke, K., Heinke, J., Blomer, U., Ruhparwar, A., Rohde, B., Li, R. K., Haverich, A., and Martin, U. 2008. Human CMV immediate-early enhancer: a useful tool to enhance cell-type-specific expression from lentiviral vectors. J Gene Med 10, no. 1:21. Niebruegge, S., Nehring, A., Bar, H., Schroeder, M., Zweigerdt, R., and Lehmann, J. 2008. Cardiomyocyte production in mass suspension culture: embryonic stem cells as a source for great amounts of functional cardiomyocytes. Tissue Eng Part A 14, no. 10:1591. Mauritz, C., Schwanke, K., Reppel, M., Neef, S., Katsirntaki, K., Maier, L. S., Nguemo, F., Menke, S., Haustein, M., Hescheler, J., Hasenfuss, G., and Martin, U. 2008. Generation of functional murine cardiac myocytes from induced pluripotent stem cells. Circulation 118, no. 5:507. Martin, U. 2008. Methods for studying stem cells: Adult stem cells for lung repair. Methods 45, no. 2:121. Martin, U. 2008. Pluripotent Nonhuman Primate Stem Cells for Biomedical Research, Development of Regenerative Therapies and Drug Screening. In Critical Contributions of Primate Models for Biopharmaceutical Drug Development. G. Weinbauer, editor. Waxmann, Münster. Winkler, M. E.*, Mauritz, C.*, Groos, S., Kispert, A., Menke, S., Hoffmann, A., Gruh, I., Schwanke, K., Haverich, A., and Martin, U. 2008. Serum-Free Differentiation of Murine Embryonic Stem Cells into Alveolar Type II Epithelial Cells. Cloning Stem Cells 10, no. 1:49. *Authors contributed equally. Wunderlich, S.*, Gruh, I.*, Winkler, M. E., Beier, J., Radtke, K., Schmiedl, A., Groos, S., Haverich, A., and Martin, U. 2008. Type II pneumocyte-restricted green fluorescent protein expression after lentiviral transduction of lung epithelial cells. Hum Gene Ther 19, no. 1:39. *Authors contributed equally. Xu, X. Q., Graichen, R., Soo, S. Y., Balakrishnan, T., Rahmat, S. N., Sieh, S., Tham, S. C., Freund, C., Moore, J., Mummery, C., Colman, A., Zweigerdt, R., and Davidson, B. P. 2008. Chemically defined medium supporting cardiomyocyte differentiation of human embryonic stem cells. Differentiation 76, no. 9:958. Xu, X. Q., Zweigerdt, R., Soo, S. Y., Ngoh, Z. X., Tham, S. C., Wang, S. T., Graichen, R., Davidson, B., Colman, A., and Sun, W. 2008. Highly enriched cardiomyocytes from human embryonic stem cells. Cytotherapy 10, no. 4:376. 2007 Crook, J. M., Peura, T. T., Kravets, L., Bosman, A. G., Buzzard, J. J., Horne, R., Hentze, H., Dunn, N. R., Zweigerdt, R., Chua, F., Upshall, A., and Colman, A. 2007. The Generation of Six Clinical-Grade Human Embryonic Stem Cell Lines. Cell Stem Cell 1, no. 5:490. Dai, W., Field, L. J., Rubart, M., Reuter, S., Hale, S. L., Zweigerdt, R., Graichen, R. E., Kay, G. L., Jyrala, A. J., Colman, A., Davidson, B. P., Pera, M., and Kloner, R. A. 2007. Survival and maturation of human embryonic stem cell-derived cardiomyocytes in rat hearts. J Mol Cell Cardiol 43, no. 4:504. Ruhparwar, A., Er, F., Martin, U., Radke, K., Gruh, I., Niehaus, M., Karck, M., Haverich, A., and Hoppe, U. C. 2007. Enrichment of cardiac pacemaker-like cells: neuregulin-1 and cyclic AMP increase I(f)-current density and connexin 40 mRNA levels in fetal cardiomyocytes. Med Biol Eng Comput 45, no. 2:221. Zweigerdt, R. 2007. The art of cobbling a running pump--will human embryonic stem cells mend broken hearts? Semin Cell Dev Biol 18, no. 6:794. 2006 Bara, C.*, Ghodsizad, A.*, Niehaus, M.*, Makoui, M., Piechaczek, C., Martin, U., Warnecke, G., Karck, M., Gams, E., Klein, H. M., Haverich, A., and Ruhparwar, A. 2006. In Vivo Echocardiographic Imaging of Transplanted Human Adult Stem Cells in the Myocardium Labeled with Clinically Applicable CliniMACS Nanoparticles. J Am Soc Echocardiogr 19, no. 5:563. *Authors contributed equally. Donath, S., Li, P., Willenbockel, C., Al-Saadi, N., Gross, V., Willnow, T., Bader, M., Martin, U., Bauersachs, J., Wollert, K. C., Dietz, R., and von Harsdorf, R. 2006. Apoptosis repressor with caspase recruitment domain is required for cardioprotection in response to biomechanical and ischemic stress. Circulation 113, no. 9:1203. Gruh, I., Beilner, J., Blomer, U., Schmiedl, A., Schmidt-Richter, I., Kruse, M. L., Haverich, A., and Martin, U. 2006. No evidence of transdifferentiation of human endothelial progenitor cells into cardiomyocytes after coculture with neonatal rat cardiomyocytes. Circulation 113, no. 10:1326. Horn, P. A., Tani, K., Martin, U., and Niemann, H. 2006. Nonhuman primates: embryonic stem cells and transgenesis. Cloning Stem Cells 8, no. 3:124. Li, Y., Koster, T., Morike, C., v Horsten, S., Martin, U., Bader, M., Haverich, A., and Simon, A. R. 2006. Pravastatin prolongs graft survival in an allogeneic rat model of orthotopic single lung transplantation. Eur J Cardiothorac Surg 30, no. 3:515. Ruhparwar, A., Bara, C., Kofidis, T., Ruebesamen, N., Karck, M., Martin, U., and Haverich, A. 2006. In Vivo Detection of Integration of Grafted Cells after Myocardial Transplantation. Zentralbl Chir 131, no. 5:420. Ruhparwar, A., Ghodsizad, A., Niehaus, M., Bara, C., Lotz, J., Voelkel, T., Makoui, M., Martin, U., Wolf, F., Gams, E., Klein, M., and Haverich, A. 2006. Clinically applicable 7-Tesla magnetic resonance visualization of transplanted human adult stem cells labeled with CliniMACS nanoparticles. Thorac Cardiovasc Surg 54, no. 7:447. Schwanke, K., Wunderlich, S., Reppel, M., Winkler, M. E., Matzkies, M., Groos, S., Itskovitz-Eldor, J., Simon, A. R., Hescheler, J., Haverich, A., and Martin, U. 2006. Generation and characterization of functional cardiomyocytes from rhesus monkey embryonic stem cells. Stem Cells 24, no. 6:1423. Schwarzer, M., Carnwath, J. W., Lucas-Hahn, A., Lemme, E., Kues, W. A., Wachsmann, B., Haverich, A., Martin, U., and Niemann, H. 2006. Isolation of bovine cardiomyocytes for reprogramming studies based on nuclear transfer. Cloning Stem Cells 8, no. 3:150. 2005 Amit, M., Winkler, M. E., Menke, S., Bruning, E., Buscher, K., Denner, J., Haverich, A., Itskovitz-Eldor, J., and Martin, U. 2005. No evidence for infection of human embryonic stem cells by feeder cell-derived murine leukemia viruses. Stem Cells 23, no. 6:761. Blomer, U., Gruh, I., Witschel, H., Haverich, A., and Martin, U. 2005. Shuttle of lentiviral vectors via transplanted cells in vivo. Gene Ther 12, no. 1:67. Gruh, I., Schwanke, K., Wunderlich, S., Bloemer, U., Scherr, M., Ganser, A., Haverich, A., and Martin, U. 2005. Shuttle system allowing simplified cloning of expression cassettes into 'advanced' generation lentiviral vectors. Biotechniques 38, no. 4:530. Schroeder, M., Niebruegge, S., Werner, A., Willbold, E., Burg, M., Ruediger, M., Field, L. J., Lehmann, J., and Zweigerdt, R. 2005. Differentiation and lineage selection of mouse embryonic stem cells in a stirred bench scale bioreactor with automated process control. Biotechnol Bioeng 92, no. 7:920. Simon, A. R., Dalla-Riva, C., Kuhn, C., Tessmann, R., Meder, I., Martin, U., and Haverich, A. 2005. Adhesive functions of both chains of VLA-integrins are not fully conserved across the human-porcine species barrier: implications for xenotransplantation. Xenotransplantation 12, no. 6:473. Ruhparwar, A., Kofidis, T., Ruebesamen, N., Karck, M., Haverich, A., and Martin, U. 2005. Intra-vital fluorescence microscopy for intra-myocardial graft detection following cell transplantation. Int J Cardiovasc Imaging 21, no. 5:569. Winkler, M. E., Wiebe, K., Burian, R., Loss, M., Appiah, R., Lorenz, R., Przemeck, M., Sommer, S., Denner, J., Winkler, M., and Martin, U. 2005. Analysis of pig-to-human PERV transmission in a triple-species kidney and lung xenotransplantation model. Transplant Int 17, no. 12. 2003 Simon, A. R., Templin, C., Schröder, C., Laaf, G., Tessmann, R., Winkler, M.E., Tacke, S., Denner, J., Lapin, B., Steinhoff, G., Patience, C., Agrba, V.Z., Chikobava, M., Haverich, A., and Martin, U. 2003. No evidence for productive PERV infection of baboon cells in an in vivo infection model. Ann Transplant 8, no. 3:24. Solter, D., Beyleveld, D., Friele, M. B., Holowka, J., Lilie, H., Lovell-Badge, R., Mandla, C., Martin, U., and Pardo Avellaneda, R. 2003. Embryo Research in a pluralistic Europe. 1 ed. Wissenschaftsethik und Technikfolgenbeurteilung, ed. C.F. Gethmann, 21. Springer, Berlin. Zandstra, P. W., Bauwens, C., Yin, T., Liu, Q., Schiller, H., Zweigerdt, R., Pasumarthi, K. B., and Field, L. J. 2003. Scalable production of embryonic stem cell-derived cardiomyocytes. Tissue Eng 9, no. 4:767. Zweigerdt, R., Burg, M., Willbold, E., Abts, H., and Ruediger, M. 2003. Generation of confluent cardiomyocyte monolayers derived from embryonic stem cells in suspension: a cell source for new therapies and screening strategies. Cytotherapy 5, no. 5:399. 2002 Blusch, J. H., Patience, C., and Martin, U. 2002. Pig endogenous retroviruses and xenotransplantation. Xenotransplantation 9, no. 4:242. Haverich, A., Mertsching, H., and Martin, U. 2002. Differentiation potential of human stem cells--approaches to tissue engineering and to cell transplantation. Z Arztl Fortbild Qualitatssich 96, no. 6-7:440. Kofidis, T., Akhyari, P., Boublik, J., Theodorou, P., Martin, U., Ruhparwar, A., Fischer, S., Eschenhagen, T., Kubis, H. P., Kraft, T., Leyh, R., and Haverich, A. 2002. In vitro engineering of heart muscle: Artificial myocardial tissue. J Thorac Cardiovasc Surg 124, no. 1:63. Martin, U. 2002. Infektion humaner Zellen durch porcine endogene Retroviren: Nur ein Artefakt immortalisierter Zellinien? In Xenotransplantation, Grundlagen, Chancen, Risiken. H. Grimm, editor. Schattauer-Verlag, Stuttgart. Martin, U. 2002. Untersuchung potentieller PERV-Transmission in subhumanen Primatenmodellen. In Xenotransplantation, Grundlagen, Chancen, Risiken. H. Grimm, editor. Schattauer-Verlag, Stuttgart. Martin, U., Tacke, S. J., Simon, A. R., Schroder, C., Wiebe, K., Lapin, B., Haverich, A., Denner, J., and Steinhoff, G. 2002. Absence of PERV specific humoral immune response in baboons after transplantation of porcine cells or organs. Transpl Int 15, no. 7:361. Simon, A. R., Schroder, C., Martin, U., Tessmann, R., Templin, C., Laaf, G., Wiebe, K., Steinhoff, G., Lapin, B., Chikobava, M., and Haverich, A. 2002. Induction of long-term peripheral microchimerism in non-human primates in a model of xenogeneic peripheral tolerance induction. Ann Transplant 7, no. 3:40. Templin, C., Simon, A. R., Schröder, C., Winkler, M.E., Laaf, G., Chikobava, M., Agrba, V.Z., Lapin, B., Haverich, A., and Martin, U. 2002. Analyse potentieller PERV-Infektionen in einem klinisch relevanten Schwein-auf-Pavian Zelltransplantationsmodell. TxMed 14, no. 4. 2001 Blusch, J.H., and Martin, U. 2001. Porcine endogene Retroviren (PERV) und Xenotransplantation. TranplantLinc 2:4. Loss, M., Arends, H., Winkler, M., Przemeck, M., Steinhoff, G., Rensing, S., Kaup, F. J., Hedrich, H. J., Winkler, M. E., and Martin, U. 2001. Analysis of potential porcine endogenous retrovirus (PERV) transmission in a whole-organ xenotransplantation model without interfering microchimerism. Transpl Int 14, no. 1:31. Mertsching, H., and Martin, U. 2001. Zelltransplantation und Tissue Engineering: Aktuelle Entwicklungen und klinische Perspektiven. Laborwelt, no. 1:4. Mertsching, H., and Martin, U. 2001. Zelltransplantation und Gewebezucht-Tissue-Engineering künstlicher Organe. biologenheute 455:2. Schroder, C., Martin, U., Simon, A., Laaff, G., Wiebe, K., Haverich, A., Lapin, B., and Steinhoff, G. 2001. Discordant lung xenotransplantation using alpha-GAL columns, pig-kidney adsorption, and complement depletion in baboons. Transplant Proc 33, no. 1-2:738. Simon, A. R., Schroder, C., Martin, U., Chikobava, M., Templin, C., Laaf, G., Wiebe, K., Lapin, B., Haverich, A., and Steinhoff, G. 2001. Induction of long-term chimerism in a pig-to-primate model of peripheral tolerance induction. Transplant Proc 33, no. 1-2:705. Templin, C., Schroder, C., Simon, A. R., Laaff, G., Kohl, J., Chikobava, M., Lapin, B., Winkler, M. E., Wiebe, K., Steinhoff, G., and Martin, U. 2001. Long-term monitoring of xenotransplanted baboons: no evidence for pig endogenous retrovirus transmission. Transplant Proc 33, no. 1-2:692. Tessmann, R., Steinhoff, G., Martin, U., Haverich, A., and Simon, A. R. 2001. In vivo differentiation of human lymphocytes in a porcine microenvironment. Transplant Proc 33, no. 1-2:792. 2000 Blusch, J. H., Patience, C., Takeuchi, Y., Templin, C., Roos, C., Von Der Helm, K., Steinhoff, G., and Martin, U. 2000. Infection of nonhuman primate cells by pig endogenous retrovirus. J Virol 74, no. 16:7687. Dalla Riva, C., Tessmann, R., Kuehn, C., Martin, U., Diehl, A., Steinhoff, G., Haverich, A., and Simon, A. R. 2000. VLA-Integrin beta-1 chain function is not fully conserved between the human and porcine species: implications for xenotransplantation. Transplant Proc 32, no. 5:1054. Kuhn, C., Tesmann, R., DallaRiva, C., Glaubitz, M., Martin, U., Steinhoff, G., Haverich, A., and Simon, A. R. 2000. In vitro differentiation of human lymphocytes in a porcine microenvironment: implication for xenogeneic organ transplantation. Transplant Proc 32, no. 5:1043. Laaf, G., Schuster, S., Martin, U., Steinhoff, G., Haverich, A., and Simon, A. R. 2000. Development of a donor-specific, automated, and cost-effective cytotoxicity assay for human serum on primary porcine cells. Transplant Proc 32, no. 5:867. Martin, U., Winkler, M.E., Id, M., Radeke, H., Arseniev, L., Takeuchi, Y., Simon, A.R., Patience, C., Haverich, A., and Steinhoff, G. 2000. Productive Infection of Primary Human Endothelial Cells by Pig Endogenous Retrovirus (PERV). Xenotransplantation 7, no. 2:138. Martin, U., Winkler, M. E., Id, M., Radecke, H., Arseniev, L., Groteluschen, R., Simon, A. R., and Steinhoff, G. 2000. Transmission of pig endogenous retrovirus to primary human cells. Transplant Proc 32, no. 5:1157. Schroder, C., Martin, U., Simon, A., Laaff, G., Wiebe, K., Galanina, O., Haverich, A., Lapin, B., and Steinhoff, G. 2000. Comparison of immunoadsorption by GAL-alpha 1,3- Gal-paa disaccharide columns and by extracorporeal kidney perfusion in the setting of discordant xenogeneic lung transplantation. Transplant Proc 32, no. 5:879. Simon, A. R., Schroder, C., Martin, U., Chikobava, M., Templin, C., Laaf, G., Kohl, J., Lapin, B., Haverich, A., and Steinhoff, G. 2000. An attempt to induce peripheral tolerance in a pig-to-primate transplantation model by infusion of ultrahigh numbers of donor peripheral blood mononuclear cells: first promising results. Transplant Proc 32, no. 5:1052. Templin, C., Schroder, C., Simon, A. R., Laaff, G., Kohl, J., Chikobava, M., Lapin, B., Steinhoff, G., and Martin, U. 2000. Analysis of potential porcine endogenous retrovirus transmission to baboon in vitro and in vivo. Transplant Proc 32, no. 5:1163. Winkler, M. E., Martin, U., Loss, M., Arends, H., Rensing, S., Kaup, F. J., Hedrich, H. J., Klempnauer, J., and Winkler, M. 2000. Porcine endogenous retrovirus is not transmitted in a discordant porcine-to-cynomolgus xenokidney transplantation model with long-term survival of organ recipients. Transplant Proc 32, no. 5:1162. 1999 Martin, U., Blusch, J., Haverich, A., von der Helm, K., and Steinhoff, G. 1999. Produktive Infektion humaner Zellen durch porcine Retroviren (PERV) aus primären Endothelzellen. Tx Med 11, no. 3:217. Martin, U., and Steinhoff, G. 1999. Porcine endogenous retroviruses (PERV): in vitro artifact or a big problem for xenotransplantation? DTW Dtsch Tierarztl Wochenschr 106, no. 4:146. Martin, U., Steinhoff, G., Kiessig, V., Chikobava, M., Anssar, M., Morschheuser, T., Lapin, B., and Haverich, A. 1999. Porcine endogenous retrovirus is transmitted neither in vivo nor in vitro from porcine endothelial cells to baboons. Transplant Proc 31, no. 2B:913. Winkler, M., Loss, M., Arends, H., Martin, U., and Klempnauer, J. 1999. Untersuchungen zur Sicherheit von Xenotransplantaten unter Einsatz von Überlebensmodellen nach diskordanter Xeno-Nierentransplantation im Primaten. Langenbecks Arch Chir:1135. 1998 Martin, U., Kiessig, V., Blusch, J. H., Haverich, A., von der Helm, K., Herden, T., and Steinhoff, G. 1998. Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cells. Lancet 352, no. 9129:692. Martin, U., Steinhoff, G., Kiessig, V., Chikobava, M., Anssar, M., Morschheuser, T., Lapin, B., and Haverich, A. 1998. Porcine endogenous retrovirus (PERV) was not transmitted from transplanted porcine endothelial cells to baboons in vivo. Transpl Int 11, no. 4:247.