Services
Our services comprise a wide range of modern sequencing methods and microarray-based applications. With the help of bioinformatics, subsequently the data are processed and provided to our users. Moreover, we organize various courses that convey the necessary know-how for data evaluation to our users.
- Support with research proposals
- Advice on study design
- Provision of computing power
- Assistence with data analysis
- Provision of commercial data analysis programs
- Bioinformatic data analysis courses
- Support with integration of generated data into public databases
- Single-cell RNA sequencing
- RNA-sequencing (after ribosomal depletion)
- RNA- sequencing (after poly-A enrichment)
- RNA- sequencing (with low or degradet input)
- smallRNA- sequencing
- microRNA- sequencing (mature microRNAs)
- Microarray-based mRNA expression profiling
- RNA quality control
- DNA quality control
- Whole genome sequencing
- Exome sequencing
- Amplikon sequencing
- Target-enrichment sequencing
- Metagenomics
- ChIP sequencing *
- Epigenetic analysis *
* So far, no explicit standards have been established. The form and extent of cooperation will be discussed individually for each project. Chip-Seq results are much more pertinent in combination with an assistive technology, such as RNA-Seq or a methylation assay.
Some of the bioinformatics services do not represent standard services and can only be carried out with the appropriate capacity and a separate agreement.
- Transcriptome alignment and quantification (RNA-seq)
- Microarray analysis
- De novo Assembly (genome, transcriptome)
- SNP detection
- bisulfite sequencing analysis
- Data visualization in genome browsers
- Provision and administration of MHH Galaxy instance
- Provision of high computing power for NGS applications via a central high performance computing cluster (HPC-seq cluster) (together with the stakeholders and the ZIMt)
- DNA sequencing data processing and analysis (including quality control, trimming, mapping, and filtering)
- Long read analyses for bacterial whole genomes, metagenomics, long amplicons
- Development and maintenance of sequencing alignment pipeline for short and long reads
- Metagenomics analysis (normalization strategies, absolute abundance profiles, genomic visualization, integration of multiple samples and their visualization)
- Single-cell RNA sequencing data processing and analysis
- Implementation and development of (Single-cell RNA) sequencing analysis tools