The Experimental Trauma Surgery has once again been recognized as a Network Laboratory Musculoskeletal Regeneration in accordance with the guidelines of the Basic Research Section of the German Society for Orthopaedics and Trauma Surgery (DGOU)!
Experimental trauma surgery
Laboratory for Musculo-Skeletal Trauma & Regenerative Therapies
The Experimental Trauma Surgery Laboratory is a laboratory in which research can be carried out using the latest technology in molecular biology, histology, immunohistology, immunohistochemistry and cell biology, as well as in animal models.
This is made possible by the latest equipment, such as a flow cytometer equipped with four lasers. Measurements can be carried out here in 96-well or single mode. Among other things, this is used for the quantitative typing of human mesenchymal stem cells from the bone marrow. A fully equipped cell culture unit including sterile workbenches, incubators and microscopes is also available for this purpose.
For molecular biological methods, equipment such as a real-time PCR machine and a Qiacube for the automatic isolation and purification of nucleic acids and a homogenizer for the automatic reduction of cells, organs and hard tissues such as bone are part of the basic equipment.
The ELISA reader, which can carry out various measurements such as protein determinations, mRNA and cDNA concentrations as well as a number of ELISA kits by entering different wavelengths, is of course also available in the Experimental Trauma Surgery laboratory.
Two rotary microtomes are available for histology - for kerosene and Technovit techniques (hard section technique). Various microscopes (reflected light and transmitted light microscopy) and a digital 4K microscope are used to analyze the preparations.
Our laboratory also has its own animal operating room for mice and rats with an isoflurane anesthesia and suction unit.
You can find more detailed information about our work on the following pages. If you have any further questions, please do not hesitate to contact us personally.
