differentiation of Borrelia subspecies by LightCycler PCR. J Clin Microbiol 39: 2663-7, 2001 Wedi B, Kapp A. Value of the cellular antigen stimulation test (CAST). Dermatology 48:347-348, 1997. Wedi B, Kapp [...] EN ISO 9001:2015. Special diagnostics Allergies Total IgE, specific IgE diagnostics, IgE inhibition test, Aspergillus IgG tryptase Cellular in-vitro diagnostics (BAT, CAST, LTT, EliSpot) Autoimmune dermatoses [...] A. Rapid identification and differentiation of fungal DNA in dermatologic specimens by LightCycler PCR. J Med Microbiol 53: 1207-14, 2004 Gutzmer R, Kiehl P, Hausmann M, Kapp A, Weiss J. Postzosteric granulomas

sequencing to identify specific bacteria and fungi. In addition to expanding the range of real-time PCR methods, we are also evaluating new diagnostic methods based on next-generation sequencing (Illumina) [...] nosocomial outbreaks of pathogens. This method also allows the development of an outbreak strain-specific PCR, which can be used to generate rapid results within 24 hours in the event of an outbreak, under ideal [...] resistance patterns (e.g. multi-resistance of Gram-negative pathogens, MRGN), culture-based resistance tests sometimes provide results that are difficult to interpret. Available assays for the detection of

3-point bending test and histologically less bone and cartilage with severe blood loss was detectable in the fracture gap compared to an isolated fracture. Furthermore, using PCR arrays, qRT-PCR with TaqMan [...] different shades of red. Biomechanical evaluation The bones are analyzed using the 3-point bending test. To do this, they are placed in anteroposterior alignment on a specially designed apparatus. A punch

Forensic DNA analysis Parentage tests Paternity investigations, maternity investigations, kinship cases, deficiency cases. A parentage test is used to determine or rule out a suspected family relationship [...] unknown corpse, body parts, blood samples or specimens from pathological examinations. Identity testing can be carried out in two ways: By direct comparison with blood samples or oral cavity swabs of persons [...] material that carry microsatellite polymorphisms are analysed using the polymerase chain reaction (PCR) and the individualizing characteristics are detected by capillary electrophoresis: Various autosomal

purpose, the initially deregulated ncRNA candidates will be validated by quantitative real-time PCR (qRT-PCR) and correlated with specific patient data. Förderstiftung MHHPlus Hannover IRMI-19 / Immunprofile [...] are included who want to participate in a pulmonary function diagnosis and a fitness test, i.e. a 6-minute walk test, give a blood sample for the definition of their immune profile and answer a questionnaire [...] questionnaire (Fatigue Assessment Scale, FAS). In addition a testing of lung function and endurance (6-MWT) will be performed, as well a blood testing (FACS, ELISA, Neurodisorder assay) to quantify immunologic

Serological tests of immune parameters Location Building K3 Plasma, serum and synovial fluid tests are carried out in our Central Laboratory. For urgent inquiries, the laboratory of the Clinical Department [...] determination (RT-PCR) Helper cell count determination (FACS) Genetic HIV-1 resistance determination (sequencing of HIV-1 reverse transcriptase and protease, integrase, gp41 and tropism testing, other regions [...] Paraproteins: Immunofixation, Bence-Jones protein in urine Antinuclear antibodies (indirect fluorescence test) Anti-dsDNA antibodies: RIA, Elisa, Crithidia Anti-ENA antibodies: anti-Sm, anti-RNP, anti-SS-B, anti-SS-A

Measurement of the recipient's share of hematopoiesis after allogeneic bone marrow transplantation using qPCR-based highly sensitive chimerism analysis Cytology Central Laboratory These diagnostics are carried [...] near Node E 30625 Hannover Germany Investigations: Molecular diagnostics for acute myeloid leukemia: PCR-based detection of chromosomal translocations and genetic aberrations at initial diagnosis on request [...] defense cells against infections after allogeneic bone marrow transplantation. For information on the tests offered in the laboratory, pre-analysis, analysis conditions, reference intervals and other relevant

Cell tools. Nucleic acids tools PCR Restriction enzymatic digest Sequencing (Sanger) Next-generation sequencing SNP genotyping High Resolution Melting Gene expression (Q-RT PCR and high throughput microfluidic [...] integrated tumor core boost in the radiotherapy of large tumors) Participation in the Oligoma study (testing the efficacy and tolerability of local radiotherapy of metastases as a supplement to standard therapy

criterion for inclusion in the study is fulfilled in the case of a positive SARS-CoV-2 PCR and/or a positive antibody test and typical symptoms noticed in the past. Prior to their infection, athletes must have

of sequence amplification reactions (polymerase chain reaction, PCR) in a high-throughput process. Measurement of gene expression PCR, western blotting and double immunofluorescence methods Methylation [...] expression, DNA methylation and the detection of sequence changes in genetic material from various test materials. Isolation of sample materials Robot-based automated isolation of DNA from fresh tissue